DEACTIVATION SOLUTION USEABLE FOR MICROORGANISM DETECTION
Composition useful for, among other things, culturing and enumerating microorganisms, and associated methods.
1 . A composition comprising
an ascorbic acid compound;
at least one compound capable of deactivating an antimicrobial agent;
a compound selected from the group consisting of pyruvate, at least one salt form thereof, and at least one hydrate of any of the foregoing;
sufficient water to form a solution or dispersion of all of the components of the composition.
2 . The composition of claim 1 wherein the ascorbic acid compound is selected from the group consisting of ascorbic acid, 2-phospho ascorbic acid, 6-O-alkyl ascorbic acid, 2,6-di-O-alkenyl ascorbic acid, 3-O alkyl ascorbic acid, 2-O-α-glycopyranosyl ascorbic acid, glyceryl ascorbate, 5,5-di-O-alkyl ascorbic acid, at least one salt form of any of the foregoing, and at least one hydrate of any of the foregoing.
3 . The composition of claim 1 wherein the ascorbic acid compound is selected from the group consisting of ascorbic acid, 2-phospho ascorbic acid, at least one salt form of any of the foregoing, and at least one hydrate of any of the foregoing.
4 . The composition of claim 1 wherein the ascorbic acid compound is 2-phospho ascorbic acid, at least one salt form of thereof, or at least one hydrate of any of the foregoing.
5 . The composition of claim 1 wherein the at least one compound capable of deactivating an antimicrobial agent comprises one or more peptones, the one or more peptones optionally comprising one or more of soytone, tryptone, and porcine peptone, and further optionally being a combination of soytone, tryptone, and porcine peptone.
6 . The composition of claim 1 further comprising a polyol surfactant, wherein the polyol surfactant is optionally glycerol.
7 . The composition of claim 1 further comprising an emulsifier.
8 . The composition of claim 1 wherein after storage at 2-8 degrees C. for 1 week under ambient atmospheric conditions, the amount of ascorbic acid compound in the composition decreases by no more than 60% when compared to the amount of ascorbic acid compound present in the composition prior to storage at 2-8 degrees C. for 1 week.
9 . The composition of claim 1 wherein the composition has a pH of about 6-8.
10 . The composition of claim 1 further comprising a buffer.
11 . The composition of claim 1 further comprising a buffer wherein the buffer has a concentration of about 10 mM to about 100 mM.
12 . The composition of claim 1 comprising about 1-20 g/L of lecithin.
13 . The composition of claim 1 comprising about 1-20 g/L of pyruvate based on the weight of sodium pyruvate.
14 - 15 . (canceled)
16 . A method of culturing for one or more microorganisms comprising
sampling a surface to remove a sample of the one or more microorganisms from the surface;
contacting the sample with a composition of claim 1 ;
contacting the sample with a growth media; and
allowing at least some of the one or more microorganisms to multiply within the growth media.
17 . The method of claim 16 wherein the step of contacting the sample with a composition of any of the preceding claims occurs before the step of contacting the sample with a growth media.
18 . The method of claim 16 wherein the step of contacting the sample with a composition of any of the preceding claims occurs after the step of contacting the sample with a growth media.
19 . The method of claim 16 wherein the surface has at least one antimicrobial agent disposed thereon.
20 . The method of claim 16 wherein the growth medium comprises agar.
21 . The method of claim 16 wherein the growth medium is a thin film growth medium.
22 . The method of claim 16 wherein the microorganism is selected from the group consisting of E. coli, S. typhi, L. monocytogenes , and a Salmonella microorganism, and mixtures thereof.