IP Library › Granted Patent US 12,359,232
Granted Patent B2
US 12,359,232 · App. 17/806,046 · Granted Jul 15, 2025

Biological processes for the production of aryl sulfates

Inventors: Christian Bille Jendresen (Copenhagen Ø, DK); Alex Toftgaard Nielsen (Rungsted Kyst, DK)
Assignee: CysBio ApS
C12P11/00C12N1/20C12N9/10C12N9/1205C12N9/1229C12N9/13C12N9/16C12N9/88C12N15/09C12N15/67
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Quick Facts
Patent No.
US 12,359,232
App. No.
17/806,046
Granted
Jul 15, 2025
Kind
B2
Abstract

The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using polypeptides or recombinant cells comprising said polypeptides. More particularly, the present invention pertains to polypeptides having aryl sulfotransferase activity, recombinant host cells expressing same and processes for the production of aryl sulfates employing these polypeptides or recombinant host cells.

Claims (21)

1. An Escherichia coli bacterium for making aryl sulfates, comprising a heterologous polypeptide having aryl sulfotransferase activity; wherein the bacterium has been modified to have an increased protein expression of an ATP sulfurylase, an APS kinase, and a PAP phosphatase compared to an identical bacterium that does not carry said modification;

wherein the polypeptide having aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity to the amino acid sequence set forth in any one of SEQ ID NOs: 1 to 13;

wherein the ATP sulfurylase comprises i) a polypeptide comprising an amino acid

sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 25, and ii) a polypeptide comprising an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 26;

wherein the APS kinase comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 27; and

wherein the PAP phosphatase comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 28.

2. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 1, 6, 7, 8, or 13.

3. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 1.

4. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 6.

5. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 7.

6. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 8.

7. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO: 13.

8. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises the amino acid sequence set forth in SEQ ID NO: 1, 6, 7, 8, or 13.

9. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises the amino acid sequence set forth in SEQ ID NO: 1.

10. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises the amino acid sequence set forth in SEQ ID NO: 6.

11. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises the amino acid sequence set forth in SEQ ID NO: 7.

12. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises the amino acid sequence set forth in SEQ ID NO: 8.

13. The bacterium according to claim 1 , wherein the polypeptide having aryl sulfotransferase activity comprises the amino acid sequence set forth in SEQ ID NO: 13.

14. The bacterium according to claim 1 , wherein the ATP sulfurylase comprises i) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 25, and ii) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 26.

15. The bacterium according to claim 1 , wherein the APS kinase comprises the amino acid sequence set forth in SEQ ID NO: 27.

16. The bacterium according to claim 1 , wherein the PAP phosphatase comprises the amino acid sequence set forth in SEQ ID NO: 28.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 10, 2025
From: TECHNICAL UNIVERSITY OF DENMARK
To: CYSBIO APS
Reel/Frame 071378/0929 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 10, 2025
From: JENDRESEN, CHRISTIAN BILLE; NIELSEN, ALEX TOFTGAARD
To: DANMARKS TEKNISKE UNIVERSITET
Reel/Frame 071503/0360 →
Priority Claims (1)
EP 14182032 · Aug 22, 2014 · regional
Continuity (3)
Continuation 16943904 · Jul 30, 2020
Division 15502322
Related Publication 20220325306A1 · Oct 13, 2022
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