Antagonist antibodies against human immune checkpoint CEACAM1 (CD66a) and formulations, kits, and methods of use thereof

1. A nucleic acid molecule comprising a nucleotide sequence encoding an antibody or antigen-binding fragment thereof that specifically binds human CEACAM1 that comprises:

(a) a heavy chain variable region comprising:

(SEQ ID NO: 13)

GYIFRNYGMN,

(SEQ ID NO: 14)

WINTYTGEPTYADDFKG,

and

(SEQ ID NO: 15)

RGWLLTGGAMDY;

and

a light chain variable region

comprising:

(SEQ ID NO: 16)

RASQDIGSSLN,

(SEQ ID NO: 17)

ATSSLDS,

and

(SEQ ID NO: 18)

LQYVSSPWT;

(b) a heavy chain variable region comprising:

(SEQ ID NO: 19)

GYIFRNYGMN,

(SEQ ID NO: 20)

WINTYTGEPTYADDFKG,

and

(SEQ ID NO: 21)

DCGTSHYYAMDY;

and

a light chain variable region

comprising:

(SEQ ID NO: 22)

RASQDISNYLN,

(SEQ ID NO: 23)

YTSRLHS,

and

(SEQ ID NO: 24)

QQGNTFPLT;

or

(c) a heavy chain variable region comprising:

(SEQ ID NO: 25)

GYAFTIYLIE,

(SEQ ID NO: 26)

VINPGSGGTNYNEKFKG,

and

(SEQ ID NO: 27)

SYYYGSFAMDY;

a light chain variable region

comprising:

(SEQ ID NO: 28)

RASQDIGNYLN,

(SEQ ID NO: 29)

YTSRLHS,

and

(SEQ ID NO: 30)

QQGNTLRT.

2. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises a nucleic acid sequence encoding:

(a) a heavy chain variable region comprising SEQ ID NO: 2;

(b) a light chain variable region comprising SEQ ID NO: 4;

(c) a heavy chain variable region comprising SEQ ID NO: 6;

(d) a light chain variable region comprising SEQ ID NO: 8;

(e) a heavy chain variable region comprising SEQ ID NO: 10; or

(f) a light chain variable region comprising SEQ ID NO: 12.

3. The nucleic acid molecule of claim 2 , wherein the nucleic acid molecule comprises a nucleic acid sequence encoding:

(a) a heavy chain variable region comprising SEQ ID NO:2 and a light chain variable region comprising SEQ ID NO:4;

(b) a heavy chain variable region comprising SEQ ID NO:6 and a light chain variable region comprising SEQ ID NO:8; or

(c) a heavy chain variable region comprising SEQ ID NO: 10 and a light chain variable region comprising SEQ ID NO: 12.

4. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1, 3, 5, 7, 9, and 11.

5. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1, 3, 5, 7, 9, and 11 each with the portion encoding a leader sequence removed.

6. The nucleic acid molecule of claim 5 , wherein the portion encoding the leader sequence of SEQ ID NO: 1 is nucleotides 1-57, wherein the portion encoding the leader sequence of SEQ ID NO: 3 is nucleotides 1-60, wherein the portion encoding the leader sequence of SEQ ID NO: 5 is nucleotides 1-57, wherein the portion encoding the leader sequence of SEQ ID NO: 7 is nucleotides 1-60, wherein the portion encoding the leader sequence of SEQ ID NO: 9 is nucleotides 1-57, and wherein the portion encoding the leader sequence of SEQ ID NO: 11 is nucleotides 1-60.

7. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule is a DNA molecule or an RNA molecule.

8. The nucleic acid molecule of claim 7 , wherein the DNA molecule is a cDNA molecule.

9. A host cell comprising the nucleic acid molecule of claim 1 .