Anti-transferrin receptor antibody and uses thereof
Aspects of the disclosure relate to antibodies that bind to transferrin receptor (e.g., transferrin receptor 1) and complexes comprising the antibody covalently linked to a molecular payload. Methods of using the antibodies are also provided.
1. An antibody that binds to human transferrin receptor (TfR), wherein the antibody is a Fab and comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 101 and a light chain comprising the amino acid sequence of SEQ ID NO: 90.
2. The antibody of claim 1 , wherein the heavy chain of the antibody comprises an N-terminal pyroglutamate.
3. The antibody of claim 1 , wherein the equilibrium dissociation constant (K D ) of binding of the antibody to the transferrin receptor is in a range from 10 −11 M to 10 −6 M.
4. The antibody of claim 1 , wherein the antibody does not specifically bind to the transferrin binding site of the transferrin receptor.
5. The antibody of claim 1 , wherein the antibody is cross-reactive with extracellular epitopes of a human and non-human primate transferrin receptor.
6. The antibody of claim 1 , wherein the antibody is not covalently linked to a molecular payload.
7. A composition comprising the antibody of claim 1 and a pharmaceutically acceptable carrier.
8. The composition of claim 7 , wherein the composition is suitable for intravenous administration.
9. The composition of claim 7 , wherein the composition is suitable for intravenous infusion.
10. The composition of claim 7 , wherein the composition is a sterile injectable solution.
11. A nucleic acid comprising nucleotide sequences encoding the heavy chain and/or the light chain of the antibody of claim 1 .
12. The nucleic acid of claim 11 , comprising a nucleotide sequence encoding the heavy chain of the antibody.
13. The nucleic acid of claim 11 , comprising a nucleotide sequence encoding the light chain of the antibody.
14. The nucleic acid of claim 11 , comprising a nucleotide sequence encoding the heavy chain and the light chain of the antibody.
15. The nucleic acid of claim 12 , wherein the nucleotide sequence is operably linked to a promoter.
16. The nucleic acid of claim 13 , wherein the nucleotide sequence is operably linked to a promoter.
17. The nucleic acid of claim 14 , wherein the nucleotide sequence is operably linked to a promoter.
18. A vector comprising the nucleic acid of claim 11 .
19. The vector of claim 18 , wherein the vector is an expression vector.
20. A cell comprising the vector of claim 18 .
21. The antibody of claim 1 , wherein the antibody is not covalently linked to an oligonucleotide.