Broad spectrum kinase binding agents
Provided herein are compounds that are broad-spectrum protein kinase binding agents, detectable tracers comprising such compounds, and method of use thereof for the detection of protein kinases.
1. A broad-spectrum kinase binding agent attached to a fluorophore, comprising a kinase binding moiety of formula:
attached to the fluorophore.
2. A broad-spectrum kinase binding agent attached to a fluorophore, comprising a kinase binding moiety of formula:
attached to the fluorophore.
3. The broad-spectrum kinase binding agent of claim 1 , wherein the kinase binding moiety is attached to the fluorophore directly.
4. The broad-spectrum kinase binding agent of claim 1 , wherein the kinase binding moiety is attached to the fluorophore via a linker.
5. The broad-spectrum kinase binding agent of claim 4 , wherein the linker comprises —[(CH 2 ) 2 O] n —, wherein n is 1-20.
6. The broad-spectrum kinase binding agent of claim 5 , wherein the linker is attached to the kinase binding moiety and/or the fluorophore by an amide bond.
7. The broad-spectrum kinase binding agent of claim 1 , comprising a structure of:
wherein X is the fluorophore.
8. The broad-spectrum kinase binding agent of claim 7 , comprising a structure of:
9. The broad-spectrum kinase binding agent of claim 2 , comprising a structure of:
wherein X is the fluorophore.
10. The broad-spectrum kinase binding agent of claim 9 , comprising a structure of:
11. The broad-spectrum kinase binding agent of claim 2 , wherein the kinase binding moiety is attached to the fluorophore directly.
12. The broad-spectrum kinase binding agent of claim 2 , wherein the kinase binding moiety is attached to the fluorophore via a linker.
13. The broad-spectrum kinase binding agent of claim 12 , wherein the linker comprises —[(CH 2 ) 2 O] n —, wherein n is 1-20.
14. The broad-spectrum kinase binding agent of claim 13 , wherein the linker is attached to the kinase binding moiety and/or the fluorophore by an amide bond.