Plant Endophytic Bacteria And Methods To Control Plant Pathogens And Pests
The inventive technology generally relates to novel plant endophytic bacterial strains that may colonize discrete plant tissues, and in particular the roots of a plant and may further be engineered to express and deliver interfering RNA molecules throughout the plant.
1 . A Pseudomonas bacterial strain selected from the group consisting of: Csr-7 (ATCC Patent Deposit No. PTA-126973); and Csr-8 (ATCC Patent Deposit No. PTA-126974).
2 . A culture broth of the Pseudomonas bacterial strain of claim 1 , a concentrate thereof, or a dry product thereof.
3 . The bacterial strain of claim 1 , wherein the bacterial strain is genetically engineered to express a heterologous nucleotide sequence, operably linked to a promoter, encoding a eukaryotic-like mRNA.
4 . The bacterial strain of claim 1 , wherein the bacterial strain is genetically engineered to express a heterologous nucleotide sequence, operably linked to a promoter, encoding an inhibitory RNA molecule that inhibits expression of at least one of the following:
an endogenous gene in a plant host;
a gene of a plant host pathogen;
a gene of a pest of a plant host; and
a gene of nematode of a plant host.
5 . The bacterial strain of claim 4 , wherein the heterologous nucleotide sequence encoding an inhibitory RNA molecule comprises a nucleotide sequence according to SEQ ID NO. 1.
6 . The bacterial strain of claim 1 , wherein the bacterial strain is genetically engineered to express a heterologous nucleotide sequence, operably linked to a promoter, encoding one or more of the following:
a heterologous double stranded RNA (dsRNA) molecule configured to inhibit expression of at least one target gene; and
a heterologous engineered RNaseIII enzyme having one or more mutations that enhance generation of small interfering RNAs (siRNAs) from catalytic cutting of the dsRNA molecule; and
7 . The bacterial strain of claim 6 , wherein the heterologous nucleotide sequence encoding an engineered RNaseIII enzyme comprises a polynucleotide sequence selected from the group consisting of: SEQ ID NO. 2, 4, and 6.
8 . The bacterial strain of claim 6 , wherein said target gene comprises at least one of the following:
an endogenous gene in a plant host;
a gene of a plant host pathogen;
a gene of a pest of a plant host; and
a gene of nematode of a plant host.
9 . The bacterial strain of claim 1 , wherein bacterial strain colonizes a target host plant.
10 . The bacterial strain of claim 9 , wherein bacterial strain colonizes the roots of the target host plant.
11 . The bacterial strain of claim 1 , wherein bacterial strain persists in the roots of a target host plant.
12 . The bacterial strain of claim 6 , wherein the dsRNA or siRNAs are produced by the bacterial strain in the roots of a target host plant and distributed through the plant's intracellular architecture initiating an RNAi response in non-root tissues of the plant.
13 . The bacterial strain of claim 9 , wherein said target host plant is selected from the group consisting of: a dicot plant, or a monocot plant.
14 . The bacterial strain of claim 13 , wherein said dicot plant, or monocot plant is selected from the group consisting of: grains, corn, wheat, rice, barley, rye, oats, sorghum, millet, sunflower, safflower, cannabis, cotton, soy, canola, alfalfa, Arabidopsis , cannabis, potato, Brassica , peanut, tobacco, tropical fruits and flowers, banana, duckweed, gladiolus, sugar cane, pineapples, dates, onions, pineapple, cashews, pistachios, flowers, ornamentals, conifers, deciduous, grapes, citrus, roses, apples, peaches, strawberries, almonds, coffee, oaks, beans, legumes, watermelon, squashes, cabbage, turnip, mustard, cacti, pecans, flax, sweet potato, soybean, coconut, avocado, maize beets, cantaloupe and vegetables.