Granted Patent
B2
US 12,286,660 · App. 17/823,471 · Granted Apr 29, 2025
Microbial fermentation for the production of terpenes
Assignee:
LanzaTech, Inc.
C12P5/007C12N9/0006C12N9/1022C12N9/1025C12N9/1029C12N9/1085C12N9/1205C12N9/1229C12N9/88C12N15/52C12N15/74C12P7/42C12P9/00C12Y101/01088C12Y101/01267C12Y117/07001C12Y202/01007C12Y203/01009C12Y203/0301C12Y205/0101C12Y205/0109C12Y207/01036C12Y207/01148C12Y207/04002C12Y207/0706C12Y401/01033C12Y402/03027C12Y402/03046C12Y406/01012C12Y503/03002Y02E50/30
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Abstract
The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.
Claims (2)
1. A method for producing isoprene by providing at least one C1 compound selected from the group consisting of carbon monoxide and carbon dioxide into contact with a recombinant C1-fixing microorganism comprising a nucleic acid encoding a group of exogenous enzymes comprising thiolase, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, and HMG-CoA reductase, mevalonate kinase, phosphomevalonate kinase, mevalonate diphosphate decarboxylase, isopentenyl-diphosphate delta-isomerase, and isoprene synthase, wherein the microorganism is Clostridium or Moorella , to allow the microorganism to produce isoprene from the C1 compound, and converting the isoprene into synthetic rubber tires.
2. The method according to claim 1 , wherein the microorganism is provided with a gas comprising hydrogen.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 8, 2022
From: KOEPKE, MICHAEL
To: LANZATECH NZ, INC.
Reel/Frame 061030/0309 →
Continuity (6)
Provisional Application
61654412
· Jun 1, 2012
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