Granted Patent
B2
US 12,421,176 · App. 17/835,916 · Granted Sep 23, 2025
Biochar as a microbial carrier
Inventors:
Richard Wilson Belcher (Oxnard, CA); Han Suk Kim (Thousand Oaks, CA); Brian Buege (Centennial, CO); Mark L. Jarand (Rotorua, NZ); Michael C. Cheiky (Thousand Oaks, CA); Ronald A. Sills (Houston, TX)
Assignee:
Carbon Technology Holdings, LLC
C05F11/08C01B32/05C05D9/00C05F11/00C05F11/02C05G3/00C05G5/10C05G5/12C05G5/40C09K17/02C09K17/04C10B53/02C10B57/02C12N1/14C12N1/16C12N1/20C12N11/12C09K17/40Y02E50/10Y02P20/145Y10S71/903
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Abstract
The invention relates to a microbial delivery system where biochar acts as a carrier for microbes.
Claims (19)
1. A microbial delivery system comprising:
a biochar, wherein the biochar comprises a pore; and
a microbe, wherein the microbe is inoculated into the pore of the biochar, wherein the microbe is inoculated into the biochar using mechanical, chemical, or biological assistance to thereby move the microbe into the pore of the biochar, and wherein the microbe is inoculated into the pore of the biochar using the application of positive or negative pressure.
2. The delivery system of claim 1 , wherein the microbe is retained on the surface of the biochar through mixing the biochar and microbe together.
3. The delivery system of claim 2 , wherein the microbe is retained on the surface of the biochar by suspending the microbe in liquid and depositing the microbe on the biochar.
4. The delivery system of claim 1 , wherein the biochar is a treated biochar.
5. The delivery system of claim 4 , wherein the biochar is treated, thereby forming a treated biochar; and wherein a property of the treated biochar differs from a property of the biochar; wherein the property of the treated biochar is one of pH; hydrophobicity, hydrophilicity, ability to hold moisture, ability to retain and exchange certain types of nutrients, ion exchange capacity, physical protection from environmental hazards or protozoa, presence or absence of nutrients, micronutrients, or sources of metabolic carbon, or ability to host other symbiotic microbes or plant systems.
6. The delivery system of claim 1 , wherein the microbe is inoculated into the pore of the biochar using a surfactant.
7. The delivery system of claim 1 , wherein the microbe retained on the surface of the biochar is selected from the group consisting of: Bacillus, Pseudomonas, Rhizobium, Burkholderia, Achromobacter, Agrobacterium , Microccocus, Aereobacter, Flavobacterium, Erwinia, Klebsiella, Enterobacter, Glomus mosseae, Glomus intraradices, Aspergillus terreus , and Aspergillus niger.
8. The delivery system of claim 1 , wherein the microbe is retained on the surface of the biochar through integrated growth with the biochar.
9. A biochar, wherein the biochar comprises a pore, wherein the pore is filled with a media, wherein the media comprises a microbe, wherein the media is infused into the pore of the biochar using mechanical, chemical, or biological assistance to thereby move the microbe into the pore of the biochar, and wherein the media is infused into the pore of the biochar using positive or negative pressure.
10. The biochar of claim 9 , wherein the media is infused into the pore of the biochar using a surfactant.
11. The biochar of claim 10 , wherein the infusing comprises adding 1% surfactant to the media.
12. The biochar of claim 9 , wherein the microbe comprises a microorganism selected from the group consisting of: Bacillus, Pseudomonas, Rhizobium, Burkholderia, Achromobacter, Agrobacterium , Microccocus, Aereobacter, Flavobacterium, Erwinia, Klebsiella, Enterobacter, Glomus mosseae, Glomus intraradices, Aspergillus terreus , and Aspergillus niger.
13. The biochar of claim 9 , wherein the pore of the biochar is treated prior to infusion with the media.
14. The biochar of claim 9 , wherein the moisture content of the pore is adjusted prior to infusion with the media.
15. A method for creating an enhanced biochar, the method comprising infusing a media into a pore of a biochar, wherein the media comprises a microbe, wherein the infusing the media comprises infusing the microbe into the pore of the biochar using mechanical, chemical, or biological assistance to thereby move the microbe into the pore of the biochar, and wherein the media is infused into the pore of the biochar using positive or negative pressure.
16. The method of claim 15 , wherein the media is infused into the pore of the biochar using a surfactant.
17. The method of claim 15 , wherein the media comprises microorganisms selected from the group consisting of: Bacillus, Pseudomonas, Rhizobium, Burkholderia, Achromobacter, Agrobacterium , Microccocus, Aereobacter, Flavobacterium, Erwinia, Klebsiella, Enterobacter, Glomus mosseae, Glomus intraradices, Aspergillus terreus , and Aspergillus nigerniger.
Assignments (6)
SECURITY INTEREST
Recorded Feb 5, 2025
From: CARBON TECHNOLOGY HOLDINGS, LLC
To: ALTER DOMUS (US) LLC
Reel/Frame 070114/0775 →
RELEASE OF SECURITY INTEREST
Recorded Feb 4, 2025
From: FORTRESS CREDIT CORP.
To: CARBON TECHNOLOGY HOLDINGS, LLC
Reel/Frame 070608/0239 →
SECURITY INTEREST
Recorded Feb 12, 2024
From: CARBON TECHNOLOGY HOLDINGS, LLC
To: FORTRESS CREDIT CORP.
Reel/Frame 066552/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 23, 2023
From: CHEIKY, MICHAEL
To: COOL PLANET BIOFUELS, INC.
Reel/Frame 062449/0845 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 18, 2022
From: BELCHER, RICHARD WILSON; KIM, HAN SUK; BUEGE, BRIAN; JARAND, MARK L.; SILLS, RONALD A.
To: COOL PLANET ENERGY SYSTEMS, INC.
Reel/Frame 062133/0170 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 18, 2022
From: COOL PLANET ENERGY SYSTEMS, INC.
To: CARBON TECHNOLOGY HOLDINGS, LLC
Reel/Frame 062152/0345 →
Continuity (18)
Continuation In Part
14385986
Provisional Application
62432253
· Dec 9, 2016
Provisional Application
62344865
· Jun 2, 2016
Provisional Application
62290285
· Feb 2, 2016
Provisional Application
62271486
· Dec 28, 2015
Provisional Application
62162219
· May 15, 2015
Provisional Application
62058472
· Oct 1, 2014
Provisional Application
62058445
· Oct 1, 2014
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