Granted Patent
B2
US 12,577,529 · App. 17/842,344 · Granted Mar 17, 2026
Methods for producing neural cells
Inventors:
Luis Williams (Cambridge, MA); Vaibhav Joshi (Cambridge, MA); Graham T. Dempsey (Sudbury, MA)
Assignee:
QUIVER HOLDINGS INC.
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Abstract
The present invention provides differentiated neural cells and methods for making differentiated neural cells from pluripotent stem cells (PSC) at an industrial scale sufficient for high-throughput assays. The methods of the invention allow billions of PSCs and/or neural cells differentiated from the PSCs to be cryopreserved and expanded at multiple steps.
Claims (18)
1 . A method for producing neuronal cells, the method comprising:
obtaining pluripotent stem cells;
introducing into said cells an inducible genetic element that encodes a pro-neuronal transcription factor;
inducing expression of the genetic element in said cells, thereby causing differentiation of said cells into neuronal cells;
cryopreserving the neuronal cells;
thawing the cryopreserved neuronal cells;
maintaining the neuronal cells in in a medium supplemented with laminin at a first concentration;
replacing the medium with a second medium comprising laminin at a second concentration lower than the first concentration; and
replacing the second medium with a third medium comprising laminin at a third non-zero concentration lower than the second concentration.
2 . The method of claim 1 , wherein the transcription factor is neurogenin-2.
3 . The method of claim 1 , further comprising using the neuronal cells in a biological assay.
4 . The method of claim 2 , wherein the thawed neuronal cells are plated and cultured in laminin coated wells of a multi-well plate.
5 . The method of claim 1 , further comprising transfecting the pluripotent stem cells with a plasmid encoding a nuclease and guide RNA.
6 . The method of claim 5 , wherein the guide RNA and nuclease are used to cause at least one knock-in or knock-out mutation.
7 . The method of claim 1 , wherein the pluripotent stem cells are induced pluripotent stem cells derived from cells obtained from a subject.
8 . The method of claim 1 , further comprising introducing one or more additional genetic constructs into the differentiated neuronal cells such that they express one or more optical reporters of cellular activity and/or optical actuators of cellular activity.
9 . The method of claim 1 , further comprising the step of expanding said pluripotent stem cells prior to the induction step.
10 . The method of claim 1 , further comprising cryopreserving and thawing the obtained pluripotent stem cells prior to the induction step.
Assignments (6)
RELEASE OF SECURITY INTEREST
Recorded May 19, 2025
From: VERADIGM LLC
To: QUIVER HOLDINGS INC.; QUIVER BIOSCIENCE, INC.
Reel/Frame 071312/0291 →
CHANGE OF NAME
Recorded Feb 20, 2025
From: QUELLTX, INC.
To: QUIVER BIOSCIENCE INC.
Reel/Frame 070277/0400 →
CHANGE OF NAME
Recorded Feb 20, 2025
From: QUIVER BIOSCIENCE INC.
To: QUIVER HOLDINGS INC.
Reel/Frame 070277/0815 →
SECURITY INTEREST
Recorded Jul 28, 2023
From: QUIVER BIOSCIENCE, INC.; QUELLTX, INC.
To: VERADIGM LLC
Reel/Frame 064424/0664 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 25, 2023
From: Q-STATE BIOSCIENCES, INC.
To: QUELLTX, INC.
Reel/Frame 064382/0096 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 16, 2022
From: WILLIAMS, LUIS; JOSHI, VAIBHAV; DEMPSEY, GRAHAM T.
To: Q-STATE BIOSCIENCES, INC.
Reel/Frame 061125/0280 →
Continuity (2)
Provisional Application
63211693
· Jun 17, 2021
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