IP Library › Granted Patent US 12,421,544
Granted Patent B2
US 12,421,544 · App. 17/864,913 · Granted Sep 23, 2025

Labeling strategy for use in DNA sequencing to facilitate assembly of sequence reads into longer fragments of a genome

Inventor: Radoje Drmanac (Los Altos Hills, CA)
Assignee: Complete Genomics, Inc.
C12Q1/6874C07H21/04C07K1/047C12Q1/6806C12Q1/682C12Q1/6837C12Q1/6869G01N15/1404G01N15/1434C12Q2525/151C12Q2525/313C12Q2531/125C12Q2565/513Y10S977/778Y10S977/789Y10S977/792Y10S977/88Y10S977/882
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Quick Facts
Patent No.
US 12,421,544
App. No.
17/864,913
Filed
Jul 14, 2022
Granted
Sep 23, 2025
Kind
B2
Art Unit
1681
USPC
506/2
Abstract

This disclosure provides technology for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

Claims (39)

1. A method of sequencing a target polynucleotide, the method comprising the steps of:

obtaining a population of nucleic acids containing overlapping first-sized fragments of the target polynucleotide;

forming a plurality of second-sized fragments from the first-sized fragments, wherein:

(1) each second-sized fragment contains a shorter portion of the target polynucleotide than the first-sized fragment from which it was obtained, labeled with an oligonucleotide tag,

(2) some of the second-sized fragments contain different portions of the target polynucleotide labeled with different oligonucleotide tags, and

(3) some of the second-sized fragments contain different portions of the target polynucleotide labeled with the same oligonucleotide tag, whereby second-sized fragments labeled with the same oligonucleotide tag more often contain fragment sequences that occur within 100 kb of each other in the target polynucleotide, compared with the second-sized fragments labeled with different oligonucleotide tags;

obtaining sequence reads from at least some of the tagged second-sized fragments; and

determining complete or partial nucleotide sequences of the target polynucleotide by a process that comprises ordering and assembling sequence reads whereby reads that contain the same oligonucleotide tag sequences are grouped together.

2. The method of claim 1 , wherein the target polynucleotide is a human genome.

3. The method of claim 1 , wherein the target polynucleotide comprises genomic DNA from multiple organisms.

4. The method of claim 1 , wherein the target polynucleotide is a cDNA expression library obtained by reverse-transcribing mRNA from a biological sample.

5. The method of claim 1 , wherein the plurality of second-sized fragments constitutes 10 to 30 equivalents of the target polynucleotide.

6. The method of claim 1 , wherein the sequence reads that are obtained for the second-sized fragments are 100 to 1,000 nucleotides in length, each containing a part of the sequence of the target polynucleotide and the sequence of the respective oligonucleotide tag.

7. The method of claim 1 , wherein the median length of the second-sized fragments is 30 to 3,000 bases.

8. The method of claim 1 , wherein the probability of second-sized fragments containing fragments of the target polynucleotide having overlapping sequences labeled with the same oligonucleotide tag is less than 1%.

9. The method of claim 1 , wherein the second-sized fragments contain two fragments that have sequences that are nearby but not immediately beside each other in the target polynucleotide separated by DNA that contains a common adaptor sequence.

10. The method of claim 1 , wherein the tagged second-sized fragments are present in a single solution.

11. The method of claim 1 , wherein the tagged second-sized fragments are present in separate aliquots.

12. The method of claim 10 , wherein the tagged second-sized fragments are prepared by separately labeling second-sized fragments in a plurality of aliquots, then pooling the aliquots to form the single solution.

13. The method of claim 1 , wherein the tagged second-sized fragments are obtained from the first-sized fragments by a process that comprises replicating portions of the first-sized fragments using tagged primers.

14. The method of claim 1 , wherein the sequence reads are obtained from the tagged second-sized fragments by a process that comprises sequencing by synthesis.

15. A method of sequencing a target polynucleotide, the method comprising the steps of:

obtaining a population of nucleic acids containing overlapping first-sized fragments of the target polynucleotide;

forming a plurality of second-sized fragments from the first-sized fragments, wherein:

(1) each second-sized fragment contains a shorter portion of the target polynucleotide than the first-sized fragment from which it was obtained conjoined to a fragment labeling means;

(2) some of the second-sized fragments contain different portions of the target polynucleotide conjoined to different labeling means, and

(3) some of the second-sized fragments contain different portions of the target polynucleotide conjoined to the same labeling means, whereby second-sized fragments conjoined to the same labeling means more often contain fragment sequences that occur within 100 kb of each other in the target polynucleotide, compared with second-sized fragments conjoined to different labeling means;

obtaining sequence reads from at least some of the labeled second-sized fragments; and

determining complete or partial nucleotide sequences of the target polynucleotide by a process that comprises ordering and assembling sequence reads whereby reads obtained from second-sized fragments conjoined to the same labeling means are grouped together.

16. A library of DNA fragments prepared from a target polynucleotide and labeled to facilitate assembly of sequence reads of the target polynucleotide,

wherein the library comprises a plurality of second-sized fragments made by subfragmenting a population of overlapping first-sized fragments of the target polynucleotide, wherein:

(1) each second-sized fragment contains a shorter portion of the target polynucleotide than the first-sized fragment from which it was obtained, labeled with an oligonucleotide tag,

(2) some of the second-sized fragments contain different portions of the target polynucleotide labeled with different oligonucleotide tags, and

(3) some of the second-sized fragments contain different portions of the target polynucleotide labeled with the same oligonucleotide tag, whereby second-sized fragments labeled with the same oligonucleotide tag more often contain fragment sequences that occur within 100 kb of each other in the target polynucleotide, compared with the second-sized fragments labeled with different oligonucleotide tags;

wherein sequence reads from at least some of the tagged second-sized fragments can be assembled to obtain complete or partial nucleotide sequences of the target polynucleotide by a process that comprises ordering and assembling sequence reads whereby reads that contain the same oligonucleotide tag sequences are grouped together.

17. The library of DNA fragments according to claim 16 , wherein the library comprises 10 to 30 equivalents of the target polynucleotide.

18. The library of DNA fragments according to claim 16 , wherein the median length of the tagged second-sized fragments is 30 to 3,000 bases.

19. The library of DNA fragments according to claim 16 , wherein the tagged second-sized fragments are present in a single solution.

20. The library of DNA fragments according to claim 16 , wherein the target polynucleotide is a human genome.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 28, 2024
From: DRMANAC, RADOJE
To: CALLIDA GENOMICS, INC.
Reel/Frame 068427/0655 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 28, 2024
From: CALLIDA GENOMICS, INC
To: COMPLETE GENOMICS, INC.
Reel/Frame 068427/0785 →
Continuity (11)
Continuation 16730829 · Dec 30, 2019
Continuation 15716314 · Sep 26, 2017
Continuation 13971797 · Aug 20, 2013
Division 13954778 · Jul 30, 2013
Division 13017244 · Jan 31, 2011
Continuation 12335168 · Dec 15, 2008
Continuation 11451692 · Jun 13, 2006
Provisional Application 60725116 · Oct 7, 2005
Provisional Application 60776415 · Feb 24, 2006
Provisional Application 60690771 · Jun 15, 2005
Related Publication 20220411865A1 · Dec 29, 2022
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