Exosomal nucleic acid vaccine modularly configured to harness multiple antigen presentation mechanisms
The present invention relates to modular systems for vaccination against infectious agents that involves the delivery of, e.g., exosome-loaded, antigen-encoding mRNAs to and into cells and tissues of the immunized subject. The present invention also relates to compositions and methods for the design, preparation, manufacture, formulation, and/or use of vaccines, e.g., nucleic acid vaccines, loaded into extracellular vesicles, e.g., exosomes loaded with synthetic mRNAs encoding multiple surface and cytoplasmic antigens of interest, e.g., antigenic polypeptides derived from an infectious virus, e.g., SARS-CoV-2, designed to elicit strong humoral and cellular immune responses due to the simultaneous expression of antigens in their native state and as exosome-associated antigens.
1. An extracellular vesicle (EV)-based nucleic acid composition, comprising one or more extracellular vesicles (EVs) each comprising a polynucleotide having an open reading frame (ORF) encoding a fusion protein comprising fragments of each of a plurality of antigenic peptides.
2. The EV-based nucleic acid composition according to claim 1 , wherein the polynucleotide is a messenger RNA (mRNA).
3. The EV-based nucleic acid composition according to claim 1 , further comprising a chemical lipofection reagent or a chemical transfection reagent.
4. The EV-based nucleic acid composition according claim 1 , wherein the fusion protein is configured, or designed, to elicit, or induce, a humoral immune response and/or a cellular immune response in a human subject.
5. The EV-based nucleic acid composition according to claim 1 , further comprising a physiologically acceptable excipient.
6. The EV-based nucleic acid composition according to claim 1 , wherein the fusion protein comprises a polypeptide as it is encoded by an infectious agent's genome that is fused, or linked, to an exosomal anchor protein.
7. The EV based nucleic acid composition according to claim 1 , wherein the fusion protein comprises fragments of one or more of SARS-Co-V-2 proteins S, N, M and E.
8. The EV based nucleic acid composition according to claim 7 , wherein the fusion protein comprises the receptor binding domain (RBD) of S, the entire N protein, and soluble portions of the M and E proteins.
9. The EV based nucleic acid composition according to claim 1 , wherein the fusion protein further comprises a transmembrane domain of Lamp1.
10. The EV-based nucleic acid composition according to claim 1 , wherein the fusion protein comprises:
(i) SARS-COV-2 spike protein (the S protein) having the amino acid sequence according to SEQ ID NO:1, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO: 1;
(ii) SARS-COV-2 nucleocapsid protein (the N protein) having the amino acid sequence according to SEQ ID NO:2, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% amino acid sequence identity with SEQ ID NO: 2;
(iii) Lamp-S(ECD)-Lamp protein (the LSL protein) having the amino acid sequence according to SEQ ID NO:3, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO: 3;
(iv) Lamp-N-Lamp protein (the LNL protein) having the amino acid sequence according to SEQ ID NO:4, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:4;
(v) SARS-COV-2 membrane protein (the M protein) having the amino acid sequence according to SEQ ID NO:5, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% amino acid sequence identity with SEQ ID NO: 5;
(vi) SARS-COV-2 envelope protein (the E protein) having the amino acid sequence according to SEQ ID NO:6, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO: 6;
(vii) FKBPDD-S(ECD) protein (the ddS protein) having the amino acid sequence according to SEQ ID NO:7, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO: 7;
(viii) FKBPDD-N protein (the ddN protein) having the amino acid sequence according to SEQ ID NO:8, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:8;
(ix) SRBDNME protein having the amino acid sequence according to SEQ ID NO: 9, or a polypeptide having at least 90%, 91%, 92%, 93%, 5 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:9; or
(x) L-SRBDNME-L protein having the amino acid sequence according to SEQ ID NO:10, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:10.
11. The EV-based nucleic acid composition according to claim 10 , comprising one or more EVs each loaded with, or combined with, mRNA encoding the M protein having the amino acid sequence according to SEQ ID NO:5, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:5, with mRNA encoding the E protein having the amino acid sequence according to SEQ ID NO:6, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:6, with mRNA encoding the N protein having the amino acid sequence according to SEQ ID NO:2, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:2, and with mRNA encoding the S protein having the amino acid sequence according to SEQ ID NO:1, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO: 1.
12. The EV-based nucleic acid composition according to claim 10 , comprising one or more EVs each loaded with, or combined with, mRNA encoding the S protein having the amino acid sequence according to SEQ ID NO:1, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:1, and with mRNA encoding the SRBDNME protein having the amino acid sequence according to SEQ ID NO:9, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:9.
13. The EV-based nucleic acid composition according to claim 10 , comprising one or more EVs each loaded with, or combined with, mRNA encoding the S protein having the amino acid sequence according to SEQ ID NO:1, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO: 1, and with mRNA encoding the L-SRBDNME-L protein having the amino acid sequence according to SEQ ID NO:10, or a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% amino acid sequence identity with SEQ ID NO:10.
14. The EV-based nucleic acid composition according to claim 10 , wherein the mRNA encoding the S protein comprises a nucleotide sequence according to SEQ ID NO:11, or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% nucleotide sequence identity with SEQ ID NO:11.
15. The EV-based nucleic acid composition according to claim 10 , wherein the mRNA encoding the SRBDNME protein comprises a nucleotide sequence according to SEQ ID NO:12, or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% nucleotide sequence identity with SEQ ID NO: 12.
16. The EV-based nucleic acid composition according to claim 10 , wherein the mRNA encoding the L-SRBDNME-L protein comprises a nucleotide sequence according to SEQ ID NO:13, or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% 98%, or 99% nucleotide sequence identity with SEQ ID NO:13.
17. The EV-based nucleic acid composition according to claim 1 , wherein the extracellular vesicle is an exosome.
18. A method of making an extracellular vesicle (EV)-based nucleic acid composition according to claim 1 , the method comprising loading a polynucleotide having an open reading frame (ORF) encoding fusion protein comprising fragments of each of a plurality of antigenic peptides into one or more extracellular vesicles (EVs).
19. The method according to claim 18 , wherein the polynucleotides are pre-mixed with one or more chemical lipofection reagents, or with one or more chemical transfection reagent.
20. A method of inducing, or eliciting, an antigen specific immune response in a subject, comprising administering the EV-based composition according to claim 1 to the subject in an effective amount to produce an antigen specific immune response.