Combination of vaccines to prophylactically treat a pig
The invention pertains to a combination of a first vaccine comprising anon-replicating immunogen of porcine circovirus type 2 (PCV-2) and a non-replicating immunogen of Mycoplasma hyopneumoniae , and a second vaccine comprising a live attenuated porcine reproductive and respiratory syndrome (PRRS) virus, for use in prophylactically treating a pig against an infection with PCV-2, an infection with Mycoplasma hyopneumoniae and an infection with PRRS virus, by associated separate injection of the first vaccine and the second vaccine into a tissue of the pig at a first and a second injection site respectively, wherein the first and second injection sites are at most 5 cm apart from each other.
1 . A method of prophylactically treating a pig against an infection with porcine circovirus type 2 (PCV2), an infection with Mycoplasma hyopneumoniae and an infection with PRRS virus by injecting into a tissue of the pig at a first and a second injection site respectively, an associated separate injection of a first vaccine comprising a non-replicating immunogen of porcine circo virus type 2 (PCV2) and a non-replicating immunogen of Mycoplasma hyopneumoniae , and a second vaccine comprising a live attenuated porcine reproductive and respiratory syndrome (PRRS) virus; wherein the associated separate injection of the first vaccine and the second vaccine occur simultaneously, and wherein the first and second injection sites are at most 5 cm apart from each other.
2 . The method of prophylactically treating of claim 1 , wherein the first and second injection sites are at most 4 cm apart from each other.
3 . The method of prophylactically treating of claim 2 , wherein the first and second injection sites are at most 3 cm apart from each other.
4 . The method of prophylactically treating of claim 1 , wherein the first and second vaccine through said injection are deposited at least partly into muscular tissue of the pig.
5 . The method of prophylactically treating of claim 4 , wherein the first and second vaccine are injected either by a hypodermic syringe that extends into the muscular tissue or by a jet stream of the respective vaccines using a needle-less device, wherein the jet stream penetrates the skin of the pig.
6 . The method of prophylactically treating of claim 1 , wherein the first vaccine and the second vaccine are administered by a single dose.
7 . The method of prophylactically treating of claim 1 , wherein the non-replicating immunogen of PCV2 is recombinantly expressed protein encoded by the ORF2 gene of PCV2.
8 . The method of prophylactically treating of claim 7 , wherein the non-replicating immunogen of PCV2 is baculovirus expressed protein of PCV2.
9 . The method of prophylactically treating of claim 1 , wherein the non-replicating immunogen of Mycoplasma hyopneumoniae is a Mycoplasma hyopneumoniae bacterin.