Resistance genes associated with disease resistance in soybeans
The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using markers, genes and chromosomal intervals derived from Glycine tomentella PI441001, PI441008, PI446958, PI583970, or PI483224. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.
1 . A transgenic Glycine max plant cell having, stably incorporated into its genome, a heterologous nucleic acid molecule comprising a polynucleotide encoding a protein having at least 90% sequence identity across the full length of SEQ ID NO: 47 operably linked to a plant active promoter, wherein expression of the protein in the transgenic Glycine max plant cell enhances Asian Soy Rust (ASR) resistance of the transgenic Glycine max plant cell compared to a control plant cell not expressing the protein.
2 . A transgenic Glycine max plant having, stably incorporated into its genome, a heterologous nucleic acid molecule comprising a polynucleotide encoding a protein having at least 90% sequence identity across the full length of SEQ ID NO: 47 operably linked to a plant active promoter, wherein expression of the protein in the transgenic Glycine max plant enhances Asian Soy Rust (ASR) resistance of the transgenic Glycine max plant compared to a control plant not expressing the protein.
3 . A Glycine max seed of the transgenic Glycine max plant of claim 2 , wherein the Glycine max seed comprises the heterologous nucleic acid molecule stably incorporated into its genome.
4 . A harvested product derived from the Glycine max seed of claim 3 , wherein the harvested product comprises the heterologous nucleic acid molecule.
5 . A processed product derived from the harvested product of claim 4 , wherein the processed product is a flour, a meal, an oil, a starch, or a product derived from any of the foregoing, and wherein the processed product comprises the heterologous nucleic acid molecule.
6 . A method of producing an Asian Soybean Rust (ASR) resistant Glycine max plant comprising the steps of:
a. contacting a recipient Glycine max plant cell with a heterologous nucleic acid molecule comprising a polynucleotide encoding a protein having at least 90% sequence identity across the full length of SEQ ID NO: 47 operably linked to a plant active promoter, wherein said heterologous nucleic acid molecule is incorporated into the genome of the recipient Glycine max plant cell;
b. regenerating the recipient Glycine max plant cell into a transgenic Glycine max plant; and
c. assaying the transgenic Glycine max plant for resistance to ASR.
7 . An ASR resistant Glycine max plant obtained by the method of claim 6 , wherein the ASR resistant Glycine max plant comprises the heterologous nucleic acid molecule.
8 . The ASR resistant Glycine max plant of claim 7 , wherein the Glycine max plant is an elite Glycine max plant.
9 . The elite Glycine max plant of claim 8 , wherein the Glycine max plant further has increased resistance to any one of the following: soy cyst nematode, bacterial pustule, root knot nematode, frog eye leaf spot, phytopthora, brown stem rot, nematode, smut, Golovinomyces cichoracearum, Erysiphe cichoracearum, Blumeria graminis, Podosphaera xanthii, Sphaerotheca fuliginea, Pythium ultimum, Uncinula necator, Mycosphaerella pinodes, Magnaporthe grisea, Bipolaris oryzae, Magnaporthe grisea, Rhizoctonia solani, Phytophthora sojae, Schizaphis graminum, Bemisia tabaci, Rhopalosiphum maidis, Deroceras reticulatum, Diatraea saccharalis, Schizaphis graminum or Myzus persicae.
10 . The transgenic Glycine max plant of claim 2 , wherein said protein has at least 95% sequence identity across the full length of SEQ ID NO: 47.
11 . The transgenic Glycine max plant of claim 10 , wherein said protein has at least 98% sequence identity across the full length of SEQ ID NO: 47.
12 . The transgenic Glycine max plant of claim 11 , wherein said protein comprises SEQ ID NO: 47.
13 . The transgenic Glycine max plant of claim 10 , wherein said plant active promoter has at least 95% sequence identity across the full length of SEQ ID NO: 7.
14 . The transgenic Glycine max plant of claim 13 , wherein said promoter comprises SEQ ID NO: 7.
15 . The transgenic Glycine max plant of claim 2 , wherein said polynucleotide comprises a nucleotide sequence having at least 90% sequence identity across the full length of SEQ ID NO: 6.
16 . The transgenic Glycine max plant of claim 15 , wherein said polynucleotide comprises a nucleotide sequence having at least 95% sequence identity across the full length of SEQ ID NO: 6.
17 . The transgenic Glycine max plant of claim 15 , wherein said polynucleotide comprises a nucleotide sequence having at least 98% sequence identity across the full length of SEQ ID NO: 6.
18 . The transgenic Glycine max plant of claim 2 , wherein said Glycine max plant is an elite Glycine max plant.
19 . The transgenic Glycine max plant of claim 11 , wherein said Glycine max plant is an elite Glycine max plant.
20 . The transgenic Glycine max plant of claim 16 , wherein said Glycine max plant is an elite Glycine max plant.
21 . The method of claim 6 , wherein said polynucleotide encodes a protein having at least 95% sequence identity across the full length of SEQ ID NO: 47.
22 . The method of claim 6 , wherein said polynucleotide encodes a protein having at least 98% sequence identity across the full length of SEQ ID NO: 47.
23 . The method of claim 6 , wherein said polynucleotide comprises a nucleotide sequence having at least 90% sequence identity across the full length of SEQ ID NO: 6.
24 . The method of claim 6 , wherein said polynucleotide comprises a nucleotide sequence having at least 95% sequence identity across the full length of SEQ ID NO: 6.
25 . The method of claim 6 , wherein the plant active promoter comprises a nucleotide sequence having at least 95% sequence identity across the full length of SEQ ID NO: 7.
26 . The method of claim 21 , wherein the contacting comprises transforming the recipient Glycine max plant cell with the heterologous nucleic acid molecule.
27 . The method of claim 22 , wherein the contacting comprises transforming the recipient Glycine max plant cell with the heterologous nucleic acid molecule.
28 . The Glycine max seed of claim 3 , wherein said protein has at least 95% sequence identity across the full length of SEQ ID NO: 47.
29 . The Glycine max seed of claim 28 , wherein said protein has at least 98% sequence identity across the full length of SEQ ID NO: 47.
30 . A method of controlling Asian Soybean Rust (ASR) in a field comprising the step of planting in the field a transgenic Glycine max plant having stably incorporated into its genome a heterologous nucleic acid molecule comprising a plant active promoter operably linked to a polynucleotide encoding a protein having at least 90% sequence identity across the full length of SEQ ID NO: 47, wherein expression of said protein in said Glycine max plant enhances resistance of the plant to ASR compared to a control Glycine max plant not expressing said protein.
31 . The method of claim 30 , wherein the protein has at least 95% sequence identity across the full length of SEQ ID NO: 47.
32 . The method of claim 31 , wherein the protein has at least 98% sequence identity across the full length of SEQ ID NO: 47.
33 . The method of claim 30 , wherein the polynucleotide comprises a nucleotide sequence having at least 90% sequence identity across the full length of SEQ ID NO: 6.
34 . The method of claim 33 , wherein the polynucleotide comprises a nucleotide sequence having at least 95% sequence identity across the full length of SEQ ID NO: 6.
35 . The method of claim 30 , wherein the plant operable promoter comprises a nucleotide sequence having at least 95% sequence identity across the full length of SEQ ID NO: 7.
36 . The method of claim 30 , wherein the transgenic Glycine max plant is an elite Glycine max plant.
37 . The method of claim 31 , wherein the transgenic Glycine max plant is an elite Glycine max plant.
38 . The method of claim 32 , wherein the transgenic Glycine max plant is an elite Glycine max plant.