Crosslinking submucosal injectate system
A method of treating a lesion in a gastrointestinal tract and an injectate system are provided. The method includes injecting a crosslinkable gel into a first tissue layer, the crosslinkable gel increasing a volume of the first tissue layer. The method also includes providing a crosslinker and resecting a portion of a first tissue layer having the increased volume away from a second tissue layer creating an exposed region in a remaining portion of the first layer and leaving a portion of the gel covering at least a portion of the exposed region. The injectate system includes a crosslinkable gel and a crosslinker where the crosslinkable gel and the crosslinker form a crosslinked gel having a compressive modulus of about 10-500 kPa.
1 . An injectate system comprising:
a crosslinkable gel; and
an electrostatic crosslinker for crosslinking the gel, the crosslinkable gel and the crosslinker forming a crosslinked gel;
wherein the crosslinkable gel is selected from the group consisting of agarose, alginate, carbomer, carboxymethyl cellulose, collagen, fibronectin, chitosan, chondroitin sulfate, dextran, polyethylene glycol, polyvinylpyrolidone, silicone, poly (hydroxyethyl methacrylate), polyacrylic acid, polyacrylamide, polyvinyl alcohol, gelatin, mucin, heparin, and hyaluronan, and
wherein the electrostatic crosslinker is selected from molecules comprising divalent elements, and
wherein the crosslinkable gel and the electrostatic crosslinker are configured to remain flowable to a treatment site and to initiate crosslinking upon the electrostatic crosslinker combining with the crosslinkable gel.
2 . The injectate system of claim 1 , wherein the system is configured to deliver a gel at a pressure between about 0.25 psi and 5 psi into a portion of a gastrointestinal tract.
3 . The injectate system of claim 1 , wherein the crosslinker is selected from the group consisting of magnesium chloride (MgCl 2 ), strontium chloride (SrCl 2 ), barium chloride (BaCl 2 ), barium sulfate (BaSO 4 ), and calcium carbonate (CaCO 3 ).
4 . The injectate system of claim 1 , wherein the crosslinker comprises strontium.
5 . The injectate system of claim 1 , wherein the crosslinkable gel and the electrostatic crosslinker are mixed in a delivery system.
6 . The injectate system of claim 1 , wherein the crosslinkable gel and the electrostatic crosslinker are combined at a treatment site.
7 . The injectate system of claim 1 , further comprising one or more crosslinking stimuli.
8 . The injectate system of claim 7 , wherein the crosslinking stimuli is light activation, temperature activation, or pH activation.
9 . The injectate system of claim 1 , wherein the crosslinked gel has a compressive modulus of 10-500 kPa.
10 . The injectate system of claim 1 , wherein the crosslinked gel has a compressive modulus of 100-500 kPa.
11 . An injectate system comprising:
a crosslinkable gel comprising alginate; and
an electrostatic crosslinker for crosslinking the gel, the crosslinkable gel and the crosslinker forming a crosslinked gel;
wherein the electrostatic crosslinker is selected from molecules comprising divalent elements selected from the group consisting of magnesium, calcium, strontium, and barium, and
wherein the crosslinkable gel and the electrostatic crosslinker are configured to remain flowable to a treatment site and to initiate crosslinking upon the electrostatic crosslinker combining with the crosslinkable gel.
12 . The injectate system of claim 11 , wherein the electrostatic crosslinker is strontium.
13 . The injectate system of claim 1 , wherein the system remains at the treatment site for a period of time in the range of 24 hours to 72 hours.
14 . The injectate system of claim 1 , wherein the system penetrates submucosal fibers at the treatment site.
15 . The injectate system of claim 14 , wherein the system below a resection plane remains in submucosal fibers after resection.
16 . The injectate system of claim 11 , wherein the system remains at the treatment site for a period of time in the range of 24 hours to 72 hours.
17 . The injectate system of claim 11 , wherein the system penetrates submucosal fibers at the treatment site.
18 . The injectate system of claim 17 , wherein the system below a resection plane remains in submucosal fibers after resection.