Catalase Composition with Improved Stability and Methods
A method of removing calculus from a tooth is described comprising: applying aqueous compositions A and B to the tooth surface, wherein the composition A comprises hydrogen peroxide or a precursor thereto and composition B comprises catalase and a stabilizer comprising hydroxyl groups; and removing at least a part of the calculus from the tooth surface. Preferred stabilizers include dipropylene glycol and 1,3 propane diol. Also described are aqueous compositions and kits.
1 . A method of removing calculus from a tooth comprising:
applying aqueous compositions A and B to the tooth surface, wherein the composition A comprises hydrogen peroxide or a precursor thereto and composition B comprises catalase and a stabilizer comprising hydroxyl groups; and
removing at least a part of the calculus from the tooth surface.
2 . The method of claim 1 wherein composition B is sufficiently stable such that after storage for at least 75 days at 37° C. composition B comprises less than 50 mg/L of hydrogen peroxide when reacted with a 3% hydrogen peroxide aqueous solution.
3 . The method of claim 1 wherein the stabilizer comprises an alkylene or ether backbone and at least two hydroxyl groups.
4 . The method of claim 3 wherein the stabilizer has a molecular weight per oxygen atom of at least 32, 33, 34, 35, 36, 37, or 38 g/mole.
5 . The method of claim 1 wherein the stabilizer is miscible with deionized water.
6 . The method of claim 1 wherein the stabilizer is selected from dipropylene glycol and 1,3 propane diol.
7 . The method of claim 1 wherein the stabilizer is present in an amount ranging from 5 to 15 wt..% of the total aqueous composition.
8 . The method of claim 1 wherein composition B further comprises a buffer having a pH of 7-8.
9 . The method of claim 1 wherein the catalase is isolated from plants or fungi.
10 . The method of claim 1 wherein composition B further comprises additives including an antimicrobial.
11 . The method of claim 1 wherein composition A comprises 1 wt..% to 10 wt..% hydrogen peroxide or a precursor thereto providing 1 wt..% to 10 wt..% hydrogen peroxide.
12 . The method of claim 1 wherein composition A is applied before composition B.
13 . The method of claim 1 wherein composition B is applied before composition A.
14 . An aqueous composition for use for removing calculus from a tooth surface comprising
catalase; and
a stabilizer comprising hydroxyl groups.
15 . The aqueous composition of claim 14 wherein the stabilizer is selected from dipropylene glycol and 1,3 propane diol.
16 . The aqueous composition of claim 14 wherein after storage for at least 75 days at 37° C. the aqueous composition comprises less than 50 mg/L of hydrogen peroxide when reacted with a 3% hydrogen peroxide aqueous solution.
17 . The aqueous composition of claim 14 wherein the stabilizer is present in an amount ranging from 5 to 15 wt..% of the total aqueous composition.
18 . The aqueous composition of claim 14 wherein the composition is free of amine compounds.
19 . A kit comprising
a composition A comprises hydrogen peroxide or a precursor thereto; and
a composition B comprising catalase and a stabilizer comprising hydroxyl groups.
20 . The kit of claim 19 wherein composition B is sufficiently stable such that after storage for at least 75 days at 37° C. composition B comprises less than 50 mg/L of hydrogen peroxide when reacted with a 3% hydrogen peroxide aqueous solution.