IP Library › Granted Patent US 12,577,614
Granted Patent B2
US 12,577,614 · App. 17/998,844 · Granted Mar 17, 2026

Kits and methods for determining copy number of mouse TCR gene

Inventors: Jiaqi Huang (Rockville, MD); Yihong Yao (Rockville, MD); Lin Guo (Shanghai, CN); Jiaxu Yan (Shanghai, CN); Xin Yao (Rockville, MD); Shigui Zhu (Rockville, MD); Fei Wang (Shanghai, CN); Junfeng Wu (Shanghai, CN); Jishun Lu (Shanghai, CN); Jiaqiang Ren (Shanghai, CN); Li Zhang (Shanghai, CN)
Assignee: Shanghai AbelZeta Ltd.
C12Q1/6858A61K40/11A61K40/32A61K40/4265C12Q1/6851C12Q2600/156C12Q2600/16
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,577,614
App. No.
17/998,844
Granted
Mar 17, 2026
Kind
B2
Abstract

The present disclosure provides systems, kits and methods for determining the copy number of a mouse TCR transgene. The primers are specific to the conserved regions of the mouse TCR gene. The primers/probes provide good amplification efficiency and can quickly and accurately determine the copy number of a mouse TCR transgene.

Claims (17)

1 . A system for determining a copy number of a mouse T cell receptor (TCR) gene, the system comprising

(i) a forward primer comprising the nucleic acid sequence set forth in SEQ ID NO:1 and (ii) a reverse primer comprising the nucleic acid sequence set forth in SEQ ID NO:2, and (iii) a probe comprising a detectable label.

2 . The system of claim 1 , wherein the detectable label is a fluorescent label.

3 . The system of claim 1 , wherein the probe comprises the nucleic acid sequence set forth in SEQ ID NO: 3.

4 . The system of claim 2 , wherein the fluorescent label comprises a fluorescent reporter and a fluorescent quencher.

5 . The system of claim 4 , wherein the fluorescent reporter is at a 5′ end of the probe, and wherein the fluorescent quencher is at a 3′ end of the probe.

6 . The system of claim 4 , wherein the fluorescent reporter is at a 3′ end of the probe, and wherein the fluorescent quencher is at a 5′ end of the probe.

7 . A method for determining a copy number of a mouse T cell receptor (TCR) gene in a sample, the method comprising:

(a) contacting the sample with the system of claim 1 ; and

(b) conducting a nucleic acid amplification reaction.

8 . A kit for determining a copy number of a mouse T cell receptor (TCR) gene, the kit comprising:

(i) a forward primer comprising a nucleic acid sequence set forth in SEQ ID NO:1 and (ii) a reverse primer comprising a nucleic acid sequence set forth in SEQ ID NO:2, and (iii) a probe comprising a detectable label.

9 . The kit of claim 8 , wherein the detectable label is a fluorescent label.

10 . The kit of claim 8 , wherein the probe comprises the nucleic acid sequence set forth in SEQ ID NO: 3.

11 . The kit of claim 9 , wherein the fluorescent label comprises a fluorescent reporter and a fluorescent quencher.

12 . The method of claim 7 , wherein the mouse TCR gene is a transgene.

13 . The method of 9 , wherein the nucleic acid amplification reaction is polymerase chain reaction (PCR).

Assignments (3)
CORRECTIVE ASSIGNMENT TO CORRECT THE RECEIVING PARTY DATA PREVIOUSLY RECORDED AT REEL: 67128 FRAME: 44. ASSIGNOR(S) HEREBY CONFIRMS THE CHANGE OF NAME. Recorded May 7, 2024
From: SHANGHAI CELLULAR BIOPHARMACEUTICAL GROUP LTD.
To: SHANGHAI ABELZETA LTD.
Reel/Frame 067334/0083 →
CHANGE OF NAME Recorded Apr 16, 2024
From: SHANGHAI CELLULAR BIOPHARMACEUTICAL GROUP LTD.
To: SHANGHAI ABELZETA LTD.
Reel/Frame 067128/0044 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 15, 2022
From: HUANG, JIAQI; YAO, YIHONG; GUO, LIN; YAN, JIAXU; YAO, XIN; ZHU, SHIGUI; WANG, FEI; WU, JUNFENG; LU, JISHUN; REN, JIAQIANG; ZHANG, LI
To: SHANGHAI CELLULAR BIOPHARMACEUTICAL GROUP LTD.
Reel/Frame 061772/0862 →
Priority Claims (1)
CN 202010423994.3 · May 18, 2020 · national
Continuity (1)
Related Publication 20230193370A1 · Jun 22, 2023
References Cited (26)
US 6773882B2 · Hogan · 2004 [cited by examiner]
US 9113616B2 · Macdonald et al. · 2015 [cited by applicant]
US 9279122B2 · Jakobsen et al. · 2016 [cited by applicant]
US 20130288237A1 · Robins et al. · 2013 [cited by applicant]
US 20170290858A1 · Zhao et al. · 2017 [cited by applicant]
US 20180327473A1 · He et al. · 2018 [cited by applicant]
US 20200131564A1 · Jiang et al. · 2020 [cited by applicant]
CN 102083999A · 2011 [cited by applicant]
CN 102618646A · 2012 [cited by applicant]
CN 105377886A · 2016 [cited by applicant]
CN 107829145A · 2018 [cited by applicant]
CN 107858400A · 2018 [cited by applicant]
CN 110117675A · 2019 [cited by applicant]
JP 2017536825A1 · 2017 [cited by applicant]
JP 2019535313A1 · 2019 [cited by applicant]
WO 2006016113A1 · 2006 [cited by applicant]
WO 2016085904A1 · 2016 [cited by applicant]
WO 2018208553A1 · 2018 [cited by applicant]
WO 2019010486A1 · 2019 [cited by applicant]
Diffenbach (PCR methods and Applications (1993) vol. 3, pp. S30-S37) (Year: 1993). [cited by examiner]
Roux et al (PCR Methods and Applications (1995) vol. 4, pp. s185-s194) (Year: 1995). [cited by examiner]
International Search Report and Written Opining of PCT/US2021/032744, mailed on Sep. 28, 2021. [cited by applicant]
Wright et al.: “Quantitative PCR for detection of the OT-1 transgene”: BMC Immunology, 2005, 6:20. [cited by applicant]
He et al.: “Inhibitory Effects of Anti-C II TA RNase P on Major Histocompatibility Complex Class II Antigens' Expression in Daudi Cells”, Chinese Journal of Biochemistry and Molecular Biology, 2007, 23(5): pp. 405-409. [cited by applicant]
Abramson et al., “Nucleic acid amplification technologies”, Current Opinion in Biotechnology, 1993; 4:41-47. [7 pages]. [cited by applicant]
Hsuih et al. “Novel, Ligation-Dependent PCR Assay for Detection of Hepatitis C Virus in Serum”, J. Clin. Micro. 34(3):501-507 (1996) [7 pages]. [cited by applicant]