IP Library Granted Patent US 12,655,440
Granted Patent B2
US 12,655,440 · App. 18/005,302 · Granted Jun 16, 2026

Genetically modified plants that exhibit an increase in seed yield comprising a first biotin attachment domain-containing (badc) gene homozygous for a wild-type allele, a second badc gene homozygous for a mutant allele, and homologs of the first and second badc genes

Inventors: Meghna Malik (Saskatoon, CA); Jihong Tang (West Roxbury, MA); Yuanyuan Ji (Saskatoon, CA); Venkatesh Bollina (Saskatoon, CA); Lifeng Chen (Saskatoon, CA); Marie Mykytyshyn (Saskatoon, CA); Kristi D. Snell (Belmont, MA)
Assignee: NUSEED NUTRITIONAL US INC.
C12N15/8247A01H5/10A01H6/20C07K14/415Y02A40/146
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Quick Facts
Patent No.
US 12,655,440
App. No.
18/005,302
Granted
Jun 16, 2026
Kind
B2
Abstract

A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant is provided. The genetically modified plant includes: (a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome and being homozygous for a wild-type allele: (b) a second badc gene, occurring in its natural position within the genome and being homozygous for a mutant allele; (c) one or more homologs of the first badc gene, each occurring in its natural position within the genome and being homozygous for a wild-type allele; and (d) one or more homologs of the second badc gene, each occurring in its natural position within the genome, and at least one being homozygous for a wild-type allele. The mutant allele does not encode a functional BADC protein. The increase in seed yield is at least 4%.

Claims (49)

1 . A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant from which the genetically modified plant was derived, the genetically modified plant comprising:

(a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;

(b) a second badc gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a mutant allele;

(c) one or more homologs of the first badc gene, each of the one or more homologs of the first badc gene occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele; and

(d) one or more homologs of the second badc gene, each of the one or more homologs of the second badc gene occurring in its natural position within the genome of the genetically modified plant, and at least one of the homologs of the second badc gene being homozygous for a wild-type allele, wherein:

(i) the wild-type alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene are identical to respective alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene from the progenitor plant;

(ii) the mutant allele of the second badc gene does not encode a functional BADC protein and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second badc gene from the progenitor plant;

(iii) the increase in seed yield is at least 4%;

(iv) the genetically modified plant is Brassica napus ; and

(v) the first badc gene or the one or more homologs of the first badc gene comprise Brassica napus badc1-1 of SEQ ID NO: 62 or badc1-2 of SEQ ID NO: 63.

2 . A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant from which the genetically modified plant was derived, the genetically modified plant comprising:

(a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;

(b) a second badc gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a mutant allele;

(c) one or more homologs of the first badc gene, each of the one or more homologs of the first badc gene occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele; and

(d) one or more homologs of the second badc gene, each of the one or more homologs of the second badc gene occurring in its natural position within the genome of the genetically modified plant, and at least one of the homologs of the second badc gene being homozygous for a wild-type allele, wherein:

(i) the wild-type alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene are identical to respective alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene from the progenitor plant;

(ii) the mutant allele of the second badc gene does not encode a functional BADC protein and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second badc gene from the progenitor plant;

(iii) the increase in seed yield is at least 4%;

(iv) the genetically modified plant is Brassica napus ; and

(v) the second badc gene or the one or more homologs of the second badc gene comprises one or more of Brassica napus badc3-1 of SEQ ID NO: 64, badc3-2 of SEQ ID NO: 65, badc3-3 of SEQ ID NO: 66, or badc3-4 of SEQ ID NO: 67.

3 . The genetically modified plant of claim 1 , wherein the second badc gene comprises Brassica napus badc3-2 of SEQ ID NO: 65.

4 . The genetically modified plant of claim 3 , wherein the mutant allele includes at least one of the additions, deletions, or substitutions within the first 111 codons of the second badc gene.

5 . The genetically modified plant of claim 4 , wherein both Brassica napus badc3-2 of SEQ ID NO: 65 and badc3-3 of SEQ ID NO: 66 are homozygous for mutant alleles.

6 . A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant from which the genetically modified plant was derived, the genetically modified plant comprising:

(a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;

(b) a second badc gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a mutant allele;

(c) one or more homologs of the first badc gene, each of the one or more homologs of the first badc gene occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele; and

(d) one or more homologs of the second badc gene, each of the one or more homologs of the second badc gene occurring in its natural position within the genome of the genetically modified plant, and at least one of the homologs of the second badc gene being homozygous for a wild-type allele, wherein:

(i) the wild-type alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene are identical to respective alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene from the progenitor plant;

(ii) the mutant allele of the second badc gene does not encode a functional BADC protein and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second badc gene from the progenitor plant;

(iii) the increase in seed yield is at least 4%;

(iv) the genetically modified plant is Brassica napus;

(v) the first badc gene and the one or more homologs of the first badc gene, each being homozygous for a wild-type allele, are Brassica napus badc1-1 of SEQ ID NO: 62and badc1-2 of SEQ ID NO: 63;

(vi) the second badc gene, being homozygous for a mutant allele, is Brassica napus badc3-2 of SEQ ID NO: 65;

(vii) the at least one homolog of the second badc gene, being homozygous for a wild-type allele, are Brassica napus badc3-1 of SEQ ID NO: 64 and badc3-4 of SEQ ID NO: 67; and

(viii) a remaining homolog of the second badc gene, being homozygous for a mutant allele, is Brassica napus badc3-3 of SEQ ID NO: 66.

7 . A method for producing the genetically modified plant of claim 6 from a progenitor plant comprising a first badc gene, a second badc gene, one or more homologs of the first badc gene, and one or more homologs of the second badc gene, each of the first badc gene, the second badc gene, the one or more homologs of the first badc gene, and the one or more homologs of the second badc gene being homozygous for a wild-type allele, the method comprising steps of:

(1) mutating the second badc gene in cells of the progenitor plant by making one or more additions, deletions, or substitutions of one more nucleotides relative to the wild-type allele of the second badc gene that eliminate function of the BADC protein encoded by the second badc gene that is mutated, thereby obtaining a mutated plant;

(2) conducting one more cycles of breeding of the mutated plant to obtain progeny of the mutated plant;

(3) identifying plants of the progeny in which the second badc gene is homozygous for the mutant allele, thereby obtaining second-badc-gene homozygous mutant plants; and

(4) screening the second-badc-gene homozygous mutant plants for one or more plants that have an increase in seed yield of at least 4% relative to the progenitor plant, thereby obtaining the genetically modified plant, wherein:

(i) the first badc gene and the one or more homologs of the first badc gene, each being homozygous for a wild-type allele, are Brassica napus badc1-1 of SEQ ID NO: 62 and badc1-2 of SEQ ID NO: 63;

(ii) the second badc gene, being homozygous for a mutant allele, is Brassica napus badc3-2 of SEQ ID NO: 65;

(iii) the at least one homolog of the second badc gene, being homozygous for a wild-type allele, are Brassica napus badc3-1 of SEQ ID NO: 64 and badc3-4 of SEQ ID NO: 67; and

(iv) a remaining homolog of the second badc gene, being homozygous for a mutant allele, is Brassica napus badc3-3 of SEQ ID NO: 66.

8 . The genetically modified plant of claim 1 , wherein the second badc gene or the one or more homologs of the second badc gene comprises one or more of Brassica napus badc3-1 of SEQ ID NO: 64, badc3-2 of SEQ ID NO: 65, badc3-3 of SEQ ID NO: 66, or badc3-4 of SEQ ID NO: 67.

9 . The genetically modified plant of claim 2 , wherein the second badc gene comprises Brassica napus badc3-2 of SEQ ID NO: 65.

10 . The genetically modified plant of claim 9 , wherein the mutant allele includes at least one of the additions, deletions, or substitutions within the first 111 codons of the second badc gene.

11 . The genetically modified plant of claim 10 , wherein both Brassica napus badc3-2 of SEQ ID NO: 65 and badc3-3 of SEQ ID NO: 66 are homozygous for mutant alleles.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 7, 2025
From: YIELD10 BIOSCIENCE, INC.
To: NUSEED NUTRITIONAL US INC.
Reel/Frame 070757/0010 →
SECURITY INTEREST Recorded Sep 17, 2024
From: YIELD10 BIOSCIENCE, INC.
To: NUSEED NUTRITIONAL US INC.
Reel/Frame 068614/0579 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 12, 2023
From: MALIK, MEGHNA; TANG, JIHONG; JI, YUANYUAN; BOLLINA, VENKATESH; CHEN, LIFENG; MYKYTYSHYN, MARIE; SNELL, KRISTI D.
To: YIELD10 BIOSCIENCE, INC.
Reel/Frame 062363/0469 →