Compositions and methods for preserving DNA methylation
The disclosure provides methods for storage solutions for preserving DNA methylation patterns over a period of time. The disclosure also provides for methods of using methylated DNA stored in such storage solutions.
1 . A composition comprising: a biological sample comprising a methylated DNA sequence; and a storage solution comprising water, sodium dodecyl sulphate (SDS) present in a concentration of about 0.5%, ethylenediaminetetraacetic acid (EDTA) present in a concentration of about 100 mM, and Tris(hydroxymethyl)aminomethane (Tris) present in a concentration of about 10 mM; wherein methylation patterns of the methylated DNA sequence are preserved.
2 . The composition of claim 1 , wherein the methylation pattern is preserved for at least 2 weeks.
3 . The composition of claim 1 , wherein the methylation pattern is preserved at room temperature.
4 . The composition of claim 1 , wherein the sample is a human biological sample.
5 . The composition of claim 1 , wherein the biological sample is a sample from any of: gastrointestinal tract, aerodigestive tract, respiratory tract, genitourinary tract, esophagus, stomach, colon, small intestine, pancreas, liver, oral cavity, oropharynx, trachea, bronchial tree, lung, breast or a body fluid.
6 . The composition of claim 5 , wherein the body fluid is any of: blood, urine, sputum, saliva, stool, bile, pancreatic juice, nasal secretions, tears, semen, vaginal secretions, cerebrospinal fluid, pleural fluid, peritoneal fluid, gastric juice, pericardial fluid, sweat, lymph, cyst fluid, pancreatic cyst fluid, synovial fluid, joint fluid, menstrual fluid, endometrial washing, breast aspirate, or amniotic fluid.
7 . The composition of claim 1 , wherein the storage solution is methanol free.
8 . The composition of claim 1 , wherein the methylated DNA sequence comprises a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any of the nucleotide sequences of vimentin or CCNA1 genes, or any fragments and/or complements thereof.
9 . The composition of claim 1 , wherein the methylated DNA patterns are preserved for at least 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 10 hours, 12 hours, 16 hours, 20 hours, 24 hours, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 10 days, two weeks, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 21 days, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, 30 days, 5 weeks, 6 weeks, 7 weeks, 8 weeks, 9 weeks, 10 weeks, 11 weeks, 12 weeks, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 1 year, 18 months or 2 years in the composition when stored at temperatures ranging between −30° C. to 50° C.
10 . A method of preserving the methylation pattern of methylated DNA molecule in a biological sample, comprising treating the biological sample with a storage solution, wherein the storage solution comprises water, sodium dodecyl sulphate (SDS) present in a concentration of about 0.5%, ethylenediaminetetraacetic acid (EDTA) present in a concentration of about 100 mM, and Tris(hydroxymethyl)aminomethane (Tris) present in a concentration of about 10 mM, and wherein the methylation pattern is preserved at room temperature.
11 . A composition comprising: a human biological sample comprising a methylated DNA sequence; and a storage solution comprising water, sodium dodecyl sulphate (SDS) present in a concentration of about 0.5%, ethylenediaminetetraacetic acid (EDTA) present in a concentration of about 100 mM, and Tris(hydroxymethyl)aminomethane (Tris) present in a concentration of about 10 mM; wherein methylation patterns of the methylated DNA sequence are preserved.