EXOSOME DELIVERY OF CANCER THERAPEUTICS
Chimeric expression constructs encoding an immunogenic polypeptide fused to a lactadherin domain are described. The compositions are useful for delivery of antigens to different cells, including cancer cells.
1 . An exosome composition comprising a plasmid consisting of a nucleic acid encoding an immunogenic protein fused with a lactadherin C1C2 domain.
2 . The exosome composition of claim 1 wherein the immunogenic protein is a streptococcal antigen encoded by the nucleic sequence of SEQ ID NO:1.
3 . The exosome composition of claim 1 wherein the immunogenic protein is encoded by a nucleic acid selected from SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4.
4 . The exosome composition of claim 1 wherein the lactadherin C1C2 domain is encoded by a nucleic acid having the sequence of SEQ ID NO:5.
5 . The exosome composition of claim 1 wherein the plasmid is a lentiviral expression vector consisting of Emm55 DNA and lactadherin C1C2 domain DNA.
6 . An exosome composition comprising human peripheral blood mononuclear cells transfected with an mRNA or DNA of claim 1 .
7 . A method of delivering an immunogenic protein to a cell, comprising administering the exosome composition of claim 1 to a cancer cell.
8 . The method of claim 7 wherein the cancer cell is a lymphoma, peripheral blood mononuclear, dendritic cell or cancer cell.
9 . The method of claim 8 wherein the cancer cells are transfected ex vivo.
10 . The method of claim 7 wherein the immunogenic protein has the sequence of SEQ ID NO:1.
11 . The method of claim 9 wherein the exosome isolated from the transfected cancer cells is administered to said subject.
12 . A method of treating cancer comprising obtaining beta-cell lymphoma cells transfected ex vivo with exosomes containing the expression construct of claim 5 and administering the transfected exosomes to a lymphoma cancer subject in need thereof.