METHOD AND SYSTEM FOR GENERATING A PLURALITY OF ANTIBODY SEQUENCES OF A TARGET FROM ONE OR MORE FRAMEWORK REGIONS
A method and system for generating a plurality of antibody sequences of a target from one or more framework regions based on at least one model. The model is trained on a training dataset of high binding affinity to generate the complementarity determining regions (CDR) from the received one or more framework regions (FR). The generated complementarity determining regions (CDR) from the each of the one or more framework regions (FR) are combined with the associated one or more framework regions to generate one or more regions of the target. The generated one or more regions comprises each of the received one or more framework regions (FR) and corresponding each of the generated complementarity determining regions (CDR). The generated one or more regions are concatenated to generate the plurality of antibody sequences of the target. The generated plurality of antibody sequences of the target has high binding affinity.
1 . A method for generating a plurality of antibody sequences of a target from one or more framework regions, comprising:
receiving one or more framework regions of one or more regions of an antibody sequence of the target, wherein the one or more regions of an antibody sequence comprises of one or more framework regions and one or more complementarity determining regions (CDR),
generating a plurality of complementarity determining regions (CDR) for each of the received one or more framework regions (FR), wherein the plurality of complementarity determining regions (CDR) is generated based on at least one model, and
combining the each of the received one or more framework regions (FR) and each of the generated complementarity determining regions (CDR) corresponding to each of the received one or more framework regions to generate one or more regions, and
concatenating the one or more regions to generate the plurality of antibody sequences of the target.
2 . The method of claim 1 , wherein the method comprises pre-processing a plurality of known antibody sequences of the target to generate a training dataset.
3 . The method of claim 2 , wherein pre-processing comprises of
processing the plurality of known antibody sequences of the target to identify one or more regions in the plurality of known antibody sequences, wherein each region of the one or more regions comprises of one or more known framework regions (FR) and one or more known complementarity determining regions (CDR),
padding the one or more known framework regions (FR) and one or more known complementarity determining regions (CDR) of the one or more regions with #to equalize lengths of the one or more regions of the plurality of known antibody sequences,
concatenating the padded one or more known framework regions (FR) and the one or more known complementarity determining regions (CDR),
inserting spaces between each character of the concatenated plurality of known antibody sequence to identify the antibodies, and
removing unidentified antibodies from the concatenated plurality of known antibody sequence to generate the training dataset.
4 . The method as claimed in claim 1 , wherein the at least one model comprises one of Autoregressive Convolutional Neural Network, Long Short-Term Memory (LSTM) networks, Markov model, and GPT-2 model.
5 . The method as claimed in claim 2 , wherein each of the sequences of the training dataset is converted into one-hot encoding to provide the one-hot encoded training dataset to the autoregressive CNN model for generating the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
6 . The method as claimed in claim 4 , wherein the Long Short-Term Memory (LSTM) networks comprises embedding layer to learn vocabulary of the training dataset to generate the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
7 . The method as claimed in claim 4 , wherein the Markov model extracts frequency and other parameters from the training dataset to generate the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
8 . The method as claimed in claim 4 , wherein the GPT-2 model implements one or more techniques to generate the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
9 . The method as claimed in claim 1 , the plurality of known antibody sequence of the target and the generated plurality of antibody sequences of the target has high binding affinity.
10 . A system for generating a plurality of antibody sequences of a target from one or more framework regions, comprising:
at least one server communicable coupled with at least one database, comprising of one or more processors configured to
receive one or more framework regions of one or more regions of an antibody sequence of the target, wherein the one or more regions of an antibody sequence comprises of one or more framework regions and one or more complementarity determining regions (CDR),
generate a plurality of complementarity determining regions (CDR) for each of the received one or more framework regions (FR), wherein the complementarity determining regions (CDR) is generated based on at least one model, and
combine the each of the received one or more framework regions (FR) and each of the generated complementarity determining regions (CDR) corresponding to each of the received one or more framework regions to generate one or more regions, and
concatenate the one or more regions to generate the plurality of antibody sequences of the target.
11 . The system as claimed in claim 10 , wherein the at least one server is configured to pre-process a plurality of known antibody sequence of the target to generate a training dataset.
12 . The system as claimed in claim 11 , wherein the at least one server is configured to
process the plurality of known antibody sequences of the target to identify one or more regions in the plurality of known antibody sequence, wherein each region of the one or more regions comprises of one or more known framework regions (FR) and one or more known complementarity determining regions (CDR),
pad the one or more known framework regions (FR) and one or more known complementarity determining regions (CDR) of the one or more regions with #to equalize lengths of the one or more regions of the received plurality of known antibody sequences,
concatenate the padded one or more known framework regions (FR) and the one or more known complementarity determining regions (CDR),
insert spaces between each character of the concatenated plurality of known antibody sequence to identify the antibodies, and
remove unidentified antibodies from the concatenated plurality of known antibody sequence to generate the training dataset.
13 . The system as claimed in claim 10 , wherein the at least one model comprises one of Autoregressive Convolutional Neural Network, Long Short-Term Memory (LSTM) networks, Markov model, and GPT-2 model.
14 . The system as claimed in claim 11 , wherein each of the sequences of the training dataset is converted into one-hot encoding to provide the one-hot encoded training dataset to the autoregressive CNN model for generating the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
15 . The system as claimed in claim 13 , wherein the Long Short-Term Memory (LSTM) networks comprises embedding layer to learn vocabulary of the training dataset to generate the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
16 . The system as claimed in claim 13 , the Markov model extracts frequency and other parameters from the training dataset to generate the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
17 . The system as claimed in claim 13 , wherein the GPT-2 model implements one or more techniques to generate the complementarity determining regions (CDR) from the each of the received one or more framework regions (FR).
18 . The system as claimed in claim 10 , the plurality of known antibody sequence of the target and the generated plurality of antibody sequences of the target has high binding affinity.