IP Library Granted Patent US 12,187,804
Granted Patent B2
US 12,187,804 · App. 18/067,484 · Granted Jan 7, 2025

Multivalent and multispecific 41BB-binding proteins

Inventors: Brendan P. Eckelman (Encinitas, CA); John C. Timmer (San Diego, CA); Chelsie Macedo (La Jolla, CA); Kyle S. Jones (San Marcos, CA); Abrahim Hussain (La Jolla, CA); Amir S. Razai (La Jolla, CA); Bryan Becklund (San Diego, CA); Rajay Pandit (La Jolla, CA); Mike Kaplan (La Jolla, CA); Lucas Rascon (La Jolla, CA); Quinn Deveraux (La Jolla, CA)
Assignee: Inhibrx Biosciences, Inc.
C07K16/2878C07K16/2827C07K2317/24C07K2317/31C07K2317/33C07K2317/35C07K2317/52C07K2317/569C07K2317/75C07K2317/76C07K2317/92C07K2319/00
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Quick Facts
Patent No.
US 12,187,804
App. No.
18/067,484
Granted
Jan 7, 2025
Kind
B2
Abstract

This invention relates generally to molecules that specifically engage 41BB, a member of the TNF receptor superfamily (TNFRSF). More specifically, this invention relates to multivalent and multispecific molecules that bind at least 41BB.

Claims (12)

1. An isolated polypeptide comprising at least one VHH domain that binds 41BB, wherein each VHH domain that binds 41BB comprises a CDR1, a CDR2, and a CDR3, respectively, comprising the amino acid sequences of SEQ ID NOs: 26, 27 and 28; or SEQ ID NOs: 26, 61, and 28; or SEQ ID NOs: 26, 63, and 28; or SEQ ID NOs: 26, 71, and 28; or SEQ ID NOs: 26, 73, and 28; or SEQ ID NOs: 26, 75, and 28; or SEQ ID NOs: 26, 77, and 28; or SEQ ID NOs: 26, 79, and 28; or SEQ ID NOs: 65, 61, and 28; or SEQ ID NOs: 67, 61, and 28; or SEQ ID NOs: 69, 61, and 28.

2. The isolated polypeptide of claim 1 , wherein at least one VHH domain that binds 41BB is humanized.

3. The isolated polypeptide of claim 1 , wherein at least one VHH domain that binds 41BB comprises an amino acid sequence that is at least 95% identical to an amino acid sequence selected from SEQ ID NOs: 25, 59, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 81, 82, and 83.

4. The isolated polypeptide of claim 1 , wherein at least one VHH domain that binds 41BB comprises an amino acid sequence selected from SEQ ID NOs: 25, 59, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 81, 82, and 83.

5. The isolated polypeptide of claim 1 , wherein at least one VHH domain that binds 41BB comprises the amino acid sequence of SEQ ID NO: 60.

6. The isolated polypeptide of claim 1 , wherein at least one VHH domain that binds 41BB comprises a CDR1 comprising the amino acid sequence of SEQ ID NO: 26, a CDR2 comprising the amino acid sequence of SEQ ID NO: 61, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 28.

7. The isolated polypeptide of claim 6 , wherein the polypeptide comprises one VHH domain that binds 41BB.

8. The isolated polypeptide of claim 1 , wherein the isolated polypeptide comprises an Fc region.

9. The isolated polypeptide of claim 8 , wherein the Fc region comprises an amino acid sequence that is at least 97% identical to an amino acid sequence selected from SEQ ID NOs: 1-6.

10. The isolated polypeptide of claim 8 , wherein the Fc region comprises an amino acid sequence selected from SEQ ID NOs: 1-6.

11. The isolated polypeptide of claim 8 , which forms a homodimer under physiological conditions.

12. The isolated polypeptide of claim 1 , wherein each VHH domain that binds 41BB comprises a CDR1 comprising the amino acid sequence of SEQ ID NO: 26, a CDR2 comprising the amino acid sequence of SEQ ID NO: 61, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 28.

Assignments (2)
SECURITY INTEREST Recorded Jan 14, 2025
From: INHIBRX BIOSCIENCES, INC.
To: OXFORD FINANCE LLC; OXFORD FINANCE LLC
Reel/Frame 069894/0045 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 10, 2024
From: INHIBRX, INC.
To: INHIBRX BIOSCIENCES, INC.
Reel/Frame 067679/0338 →
Continuity (4)
Division 16601825 · Oct 15, 2019
Division 15404016 · Jan 11, 2017
Provisional Application 62277028 · Jan 11, 2016
Related Publication 20230416387A1 · Dec 28, 2023
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