Method of precipitating phytase
Herein is disclosed a process for effectively precipitating phytase as a complex with a polyanion, as well as compositions comprising phytase and a polyanion, and a method for manufacturing such compositions.
1. A method of preparing a phytase composition comprising
i) providing an aqueous medium comprising phytase;
ii) preparing a reversible phytase-polyanion complex in an enzymatically active form by adding a polyanion selected from an alginic acid, a pectic acid, a hyaluronic acid, a salt thereof, or any combination thereof, to the aqueous medium; and
iii) directly recovering the reversible phytase-polyanion complex in an enzymatically active form to obtain the phytase composition;
wherein no Ca 2+ is added to the aqueous medium during the method.
2. The method of claim 1 , wherein the phytase composition is dehydrated to obtain a dry product.
3. The method of claim 1 , further comprising dissolving of the phytase composition to obtain a liquid product.
4. The method of claim 3 , further comprising dehydrating the phytase composition to obtain a dry product.
5. The method of claim 4 , further comprising dissolving the dry product to obtain a reconstituted product.
6. The method of claim 1 , further comprising washing the phytase composition.
7. The method of claim 6 , wherein the phytase composition is dehydrated to obtain a dry product.
8. The method of claim 6 , further comprising dissolving of the phytase composition to obtain a liquid product.
9. The method of claim 8 , further comprising dehydrating the phytase composition to obtain a dry product.
10. The method of claim 9 , further comprising dissolving the dry product to obtain a reconstituted product.