System and method for characterizing, monitoring, and detecting bioaerosol presence and movement in an indoor environment

1. A method comprising:

during a calibration period for an environment:

triggering collection of an initial aerosol sample, over an initial sampling period, by an air sampler located in the environment;

during the initial sampling period, triggering dispensation of a first tracer test load by a dispenser located in the environment, the first tracer test load comprising a set of tracer particles in solution;

accessing a first detected tracer level of a first tracer, in the set of tracer particles, detected in the initial aerosol sample;

accessing a first true tracer level of the first tracer present in the first tracer test load; and

deriving a first calibration factor, in a set of calibration factors, for the first tracer in the environment based on a difference between the first detected tracer level and the first true tracer level; and

during a live period succeeding the calibration period:

triggering collection of a first aerosol sample, over a first sampling period, by the air sampler;

accessing a first detected pathogen level of a first pathogen, in a set of pathogens, detected in the first aerosol sample; and

predicting a first pathogen level of the first pathogen in the first aerosol sample based on the first detected pathogen level and the first calibration factor.

2. The method of claim 1 :

wherein triggering dispensation of the first tracer test load comprising the set of tracer particles in solution comprises triggering dispensation of the first tracer test load comprising fluorescent material in solution;

wherein accessing the first detected tracer level of the first tracer detected in the initial aerosol sample comprises accessing a first detected fluorescence level of fluorescent material detected in the initial aerosol sample;

wherein accessing the first true tracer level of the first tracer present in the first tracer test load comprises accessing a first true fluorescence level of fluorescent material present in the first tracer test load; and

wherein deriving the first calibration factor for the first tracer based on the difference between the first detected tracer level and the first true tracer level comprises deriving the first calibration factor for fluorescent material in the environment based on the difference between the first detected fluorescence level and the first true fluorescence level.

3. The method of claim 1 , further comprising, in response to the first pathogen level exceeding a threshold pathogen level:

selecting a first mitigation technique, in a set of mitigation techniques, predicted to reduce the first pathogen level in the environment;

generating a prompt to execute the first mitigation technique in the environment; and

transmitting the prompt to a user affiliated with the environment.

4. The method of claim 1 , further comprising:

during the calibration period:

triggering collection of a second aerosol sample over a second sampling period by the air sampler;

during the second sampling period, triggering dispensation of a second tracer test load by the dispenser, the second tracer test load comprising a second set of tracer particles in solution;

accessing a second detected tracer level of the first tracer, in the second set of tracers, detected in the second aerosol sample;

accessing a second true tracer level of the first tracer present in the second tracer test load;

deriving a second calibration factor, in the set of calibration factors, for the first tracer in the environment based on a second difference between the second detected tracer level and the second true tracer level;

accessing a first set of environmental data recorded for the environment during the initial sampling period;

accessing a second set of environmental data recorded for the environment during the second sampling period; and

deriving a calibration model linking environmental data to the set of calibration factors in the environment based on the first calibration factor, the first set of environmental data, the second calibration factor, and the second set of environmental data; and

wherein predicting the first pathogen level of the first pathogen in the first aerosol sample based on the first detected pathogen level and the first calibration factor comprises:

accessing a third set of environmental data recorded for the environment during the first sampling period; and

predicting the first pathogen level of the first pathogen in the first aerosol sample based on the first detected pathogen level, the third set of environmental data, and the calibration model.

5. The method of claim 1 :

wherein triggering dispensation of the first tracer test load by the dispenser located in the environment comprises triggering dispensation of the first tracer test load by the dispenser installed in a first location in the environment;

wherein triggering collection of the initial aerosol sample by the air sampler located in the environment comprises triggering collection of the initial aerosol sample by the air sampler located in a second location within the environment; and

further comprising, during the calibration period, characterizing aerosol movement between the first location and the second location based on the first calibration factor.

6. The method of claim 1 , further comprising, during the live period:

triggering collection of a second aerosol sample, over a second sampling period, by the air sampler;

during the second sampling period, triggering dispensation of a second tracer test load by the dispenser, the second tracer test load comprising tracer particles in solution;

accessing a second detected tracer level of the first tracer detected in the second aerosol sample; and

in response to the second detected tracer level falling below a threshold tracer level, flagging the environment for further investigation.

7. The method of claim 1 :

wherein triggering dispensation of the first tracer test load comprising the set of tracer particles in solution comprises triggering dispensation of the first tracer test load comprising a set of barcodes in solution;

wherein accessing the first detected tracer level of the first tracer detected in the initial aerosol sample comprises accessing a first detected barcode level of a first barcode, in the set of barcodes, detected in the initial aerosol sample;

wherein accessing the first true tracer level of the first tracer present in the first tracer test load comprises accessing a first true barcode level of the first barcode present in the first tracer test load; and

wherein deriving the first calibration factor for the first tracer based on the difference between the first detected tracer level and the first true tracer level comprises deriving the first calibration factor for the first barcode based on the difference between the first detected barcode level and the first true barcode level.

8. The method of claim 7 :

wherein triggering dispensation of the first tracer test load comprising the set of barcodes in solution comprises triggering dispensation of the first tracer test load comprising the set of barcodes and fluorescent material in solution;

wherein deriving the first calibration factor, in the set of calibration factors, for the first barcode comprises deriving a first barcode calibration factor, in a set of barcode calibration factors, for the first barcode; and

further comprising:

during the calibration period:

accessing a first detected fluorescence level of fluorescent material detected at the air sampler;

accessing a first true fluorescence level of fluorescent material detected at the air sampler;

deriving a first fluorescence calibration factor, in a set of fluorescence calibration factors, for fluorescent material in the environment based on a difference between the detected fluorescence level and the first true fluorescence level; and

deriving a conversion factor based on the first barcode calibration factor and the first fluorescence calibration factor; and

during the live period:

triggering dispensation of a second tracer test load by the dispenser, the second tracer test load comprising fluorescent material in solution;

accessing a second detected fluorescence level of fluorescent material detected at the air sampler; and

estimating a corrected fluorescence level of fluorescent material at the air sampler based on the second detected fluorescence level and the conversion factor.

9. The method of claim 1 :

further comprising, during the calibration period:

accessing a second detected tracer level of a tracer barcode, in the set of tracers, detected in the initial aerosol sample;

accessing a second true tracer level of the second tracer present in the first tracer test load; and

deriving a second calibration factor, in the set of calibration factors, for the second tracer in the environment based on a difference between the second detected tracer level and the second true tracer level; and

wherein predicting the first pathogen level of the first pathogen in the first aerosol sample based on the first detected pathogen level and the first calibration factor comprises:

selecting the first calibration factor, in place of the second calibration factor, based on characteristics of the first pathogen; and

predicting the first pathogen level of the first pathogen in the first aerosol sample based on the first detected pathogen level and the first calibration factor.

10. The method of claim 9 , further comprising, during the live period:

accessing a second detected pathogen level of a second pathogen, in the set of pathogens, detected in the second aerosol sample;

selecting the second calibration factor, in place of the first calibration factor, based on characteristics of the second pathogen; and

predicting a second pathogen level of the second pathogen in the first aerosol sample based on the second detected pathogen level and the second calibration factor.

11. A method comprising:

during a calibration period:

triggering dispensation of a first tracer test load by a first dispenser installed in a first location in the environment, the first tracer test load comprising a first true tracer level of tracer molecules in solution;

accessing a first timeseries of tracer levels for tracer molecules detected at an air sampler located in the environment;

deriving a first calibration curve, in a set of calibration curves, for tracer molecules dispensed at the first location based on the first timeseries of tracer levels and the first true tracer level; and

interpreting a set of aerosol flow patterns in the environment based on the set of calibration curves; and

during a live period succeeding the calibration period:

accessing a first pathogen level of a first pathogen, in a set of pathogens, detected at the air sampler; and

based on the first pathogen level of the first pathogen and the set of aerosol flow patterns, predicting a pathogen level gradient of the first pathogen in the environment.

12. The method of claim 11 :

wherein triggering dispensation of the first tracer test load comprises triggering dispensation of the first tracer test load at a first time during the calibration period;

wherein accessing the first timeseries of tracer levels for tracer molecules detected at the air sampler comprises:

accessing a first detected tracer level of tracer molecules detected at the air sampler at a second time, succeeding the first time, within the calibration period; and

accessing a second detected tracer level of tracer molecules detected at the air sampler at a third time, succeeding the second time, within the calibration period; and

wherein deriving the first calibration curve based on the first timeseries of tracer levels and the first true tracer level comprises:

deriving a first calibration factor, in a set of calibration factors, associated with the second time, based on a first difference between the first detected tracer level and the first true tracer level;

deriving a second calibration factor, in the set of calibration factors, associated with the third time, based on a second difference between the second detected tracer level and the first true tracer level; and

deriving the first calibration curve based on the first calibration factor, associated with the second time, and the second calibration factor associated with the third time.

13. The method of claim 11 :

wherein triggering dispensation of the first tracer test load comprising the first true tracer level of tracer molecules in solution comprises triggering dispensation of the first tracer test load comprising the first true tracer level of tracer molecules of a first type in solution;

wherein accessing the first timeseries of tracer levels for tracer molecules detected at the air sampler comprises accessing the first timeseries of tracer levels for tracer molecules of the first type detected at the air sampler; and

further comprising, during the calibration period:

triggering dispensation of a second tracer test load by a second dispenser installed in a second location in the environment, the second tracer test load comprising a second true tracer level of tracer molecules of a second type in solution;

accessing a second timeseries of tracer levels for tracer molecules of the second type detected at the air sampler; and

deriving a second calibration curve, in the set of calibration curves, for tracer molecules dispensed at the second location based on the second timeseries of tracer levels and the second true tracer level.

14. The method of claim 13 :

wherein triggering dispensation of the first tracer test load comprising tracer molecules of the first type in solution comprises triggering dispensation of the first tracer test load comprising fluorescent material of a first fluorescence type in solution; and

wherein triggering dispensation of the second tracer test load comprising tracer molecules of the second type in solution comprises triggering dispensation of the second tracer test load comprising fluorescent material of a second fluorescence type in solution.

15. A method comprising:

during a calibration period:

at a first time, triggering dispensation of a first tracer test load by a dispenser located in an environment, the first tracer test load comprising a set of tracer particles in solution;

at a second time succeeding the first time, accessing a first detected tracer level of a first tracer, in the set of tracer particles, detected at an air sampler located in the environment;

accessing a first true tracer level of the first tracer present in the first tracer test load; and

deriving a first calibration factor, in a set of calibration factors, for the first tracer in the environment based on a difference between the first detected tracer level and the first true tracer level; and

during a live period succeeding the calibration period:

accessing a first detected pathogen level of a first pathogen, in a set of pathogens, detected at the air sampler; and

predicting a first pathogen level of the first pathogen in the environment based on the first detected pathogen level and the first calibration factor.

16. The method of claim 15 :

further comprising, during the calibration period, triggering collection of an initial aerosol sample, over an initial sampling period, within the calibration period, by the air sampler;

wherein accessing the first detected tracer level of the first tracer detected at the air sampler comprises accessing the first detected tracer level of the first tracer detected in the initial aerosol sample collected by the air sampler;

further comprising, during the live period, triggering collection of a first aerosol sample, over a first sampling period, within the live period, by the air sampler; and

wherein accessing the first detected pathogen level of the first pathogen detected at the air sampler comprises accessing the first detected pathogen level of the first pathogen detected in the first aerosol sample collected by the air sampler).

17. The method of claim 15 :

wherein triggering release of the first tracer test load by the dispenser comprises triggering release of the first tracer test load by the dispenser comprising:

a tracer reservoir containing tracer particles in solution;

a dispenser communication module configured to receive commands for operation of the dispenser; and

an actuator configured to release the first tracer test load from the tracer reservoir based on a command received by the dispenser communication module; and

wherein accessing the first detected tracer level of the first tracer detected at the air sampler comprises accessing the first detected tracer level of the first tracer detected at the air sampler comprising:

a sampler housing defining an air inlet and an air outlet;

a tunnel arranged within the housing and extending between the air inlet and the air outlet; and

a set of sensors configured to detect presence of tracer particles in air flowing through the tunnel.

18. The method of claim 15 , further comprising, during the calibration period:

at a third time succeeding the second time, accessing a second detected tracer level of the first tracer detected at the air sampler;

deriving a second calibration factor, in the set of calibration factors, for the first tracer in the environment based on a second difference between the second detected tracer level and the first true tracer level;

associating the first calibration factor with a first time value corresponding to the second time;

associating the second calibration factor with a second time value corresponding to the third time; and

deriving a calibration curve for the first tracer in the environment based on the first calibration factor, the first time value, the second calibration factor, and the second time value.

19. The method of claim 18 :

wherein triggering dispensation of the first tracer test load by the dispenser located in the environment comprises triggering dispensation of the first tracer test load by the dispenser located in a first location within the environment;

wherein accessing the first detected tracer level of the first tracer detected at the air sampler located in the environment comprises accessing the first detected tracer level of the first tracer detected at the air sampler located in a second location within the environment; and

further comprising, characterizing aerosol movement between the first location and the second location within the environment based on the calibration curve).

20. The method of claim 18 , further comprising, during the live period:

at a fourth time, triggering dispensation of a second tracer test load by the dispenser, the second tracer test load comprising the set of tracer molecules in solution;

accessing a third true tracer level of the first tracer present in the second tracer test load;

estimating a predicted tracer level of the first tracer in the environment at a fifth time succeeding the fourth time based on the calibration curve and the third true tracer level;

accessing a third detected tracer level of the first tracer detected at the air sampler at approximately the fifth time;

characterizing a difference between the predicted tracer level and the third detected tracer level; and

in response to the difference exceeding a threshold difference, flagging the environment for further investigation.