Method for stabilizing hemoglobin and reagents for performing the same
A stool resuspension solution comprising a hemoglobin stabilization reagent is provided. In some embodiments, the hemoglobin stabilization reagent may be an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, or an HRP stabilization component and, optionally, a polyvalent cation. A method of stabilizing hemoglobin in a stool sample in the solution is also provided, as well as a sample collection device containing the solution.
1. A stool resuspension solution effective to stabilize hemoglobin, the solution comprising:
protoporphyrin complexed with Cr 3+ , or
protoporphyrin complexed with Co 3+ ,
wherein the protoporphyrin complexed with Cr 3+ or Co 3+ is effective to stabilize hemoglobin.
2. The stool resuspension solution of claim 1 , wherein the concentration of the protoporphyrin complexed with Cr 3+ is 1.25-5 μM.
3. The stool resuspension solution of claim 1 , wherein the concentration of the protoporphyrin complexed with Co 3+ is 2.5-10 μM.
4. The stool resuspension solution of claim 1 , wherein the concentration of the protoporphyrin complexed with Cr 3+ is 1.5-4.5 μM.
5. The stool resuspension solution of claim 1 , wherein the concentration of the protoporphyrin complexed with Co 3+ is 3-5 μM.
6. The stool resuspension solution of claim 1 , wherein the protoporphyrin is protoporphyrin IX.
7. The stool resuspension solution of claim 1 , wherein the solution has a pH in the range of pH 6.5 to pH 7.4.
8. The stool resuspension solution of claim 2 , wherein the pH of the solution is in the range of pH 6.9 to pH 7.4.
9. The stool resuspension solution of claim 3 , wherein the pH of the solution is in the range of pH 6.5 to pH 7.4.
10. The stool resuspension solution of claim 1 , wherein a stool sample is suspended in the solution.
11. The stool resuspension solution of claim 1 , further comprising a buffer, bovine serum albumin, polysorbate 20, sodium azide, sodium chloride, ethylenediaminetetraacetic acid, and gentamicin.
12. A stool sample collection device comprising:
a stool sample collection container having an open end; and
a stool resuspension solution comprising protoporphyrin complexed with Cr 3+ or Co 3+ , wherein the protoporphyrin complexed with Cr 3+ or Co 3+ is effective to stabilize hemoglobin.
13. The stool sample collection device of claim 12 , further comprising a stool sampling rod comprising a distal beveled tip for scooping and/or scraping a sample of stool, wherein the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing the distal end of the sampling rod within the device.
14. The stool sample collection device of claim 13 , wherein the sample collection container and sampling rod connect via a screw fit.
15. The stool sample collection device of claim 12 , wherein the concentration of the protoporphyrin complexed with Cr 3+ is 1.25-5 μM.
16. The stool sample collection device of claim 12 , wherein the concentration of the protoporphyrin complexed with Co 3+ is 2.5-10 μM.
17. The stool sample collection device of claim 12 , wherein the concentration of the protoporphyrin complexed with Cr 3+ is 1.5-4.5 μM and wherein the concentration of the protoporphyrin complexed with Co 3+ is 3-5 μM.
18. The stool sample collection device of claim 12 , wherein the protoporphyrin is protoporphyrin IX.
19. The stool sample collection device of claim 15 , wherein the solution has a pH in the range of pH 6.5 to pH 7.4.
20. The stool sample collection device of claim 16 , wherein the pH of the solution is in the range of pH 6.9 to pH 7.4.