Monokaryon mycelial material and related method of production
A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.
1. A monokaryotic mycelium mass producing system comprising:
a culture unit for preparing a monokaryon culture wherein the culture utilizes a live culture sample of a fungal material;
a spore stock unit for creating a spore stock;
a plating unit for performing a peroxide-based spore rescue and a plating process;
a section unit for sectioning a plurality of robust hyphae;
a sub-plating unit for sub-plating and expanding the plurality of robust hyphae onto a spawn grain master;
an expanding unit for subsequently expanding the spawn grain master into production of spawn volume;
a colonization unit for performing a subsequent colonization of a mycelium substrate thereby creating a monokaryotic mass of mycelium; and
a growing space comprising a tray having a conveying platform and a permeable membrane with a plurality of pores positioned on the conveying platform.
2. The monokaryotic mycelium mass producing system of claim 1 wherein in a surface of a mass of mycelium grown within said growing space, less than 10% of said surface exhibits the formation of fruiting bodies.
3. The monokaryotic mycelium mass producing system of claim 2 wherein the plating unit utilizes the plurality of fruiting bodies for performing the spore rescue and the plating process.
4. The monokaryotic mycelium mass producing system of claim 1 wherein the environment of the growing space is such that the substrate weight to tray empty space volume ratio is between 0.5 and 5.0 g/cc, the tray empty space volume to substrate volume is between 0.01 and 1.0, and the tray empty space volume to substrate area is between 0.5 and 5.0 cc/cm 2 .
5. The monokaryotic mycelium mass producing system of claim 1 wherein within the growing space the CO 2 concentration is held above 3% in steady state conditions, the relative humidity is held above 40% in steady state conditions, and wherein the 02 concentration is held below 20% in steady state conditions to promote mycelium growth without the fruiting body.
6. The monokaryotic mycelium mass producing system of claim 1 wherein the monokaryon culture is adaptable to prepare a defect-free mass of mycelium having surface area wherein less than 1% of said surface area exhibits the formation of the fruiting bodies.
7. The monokaryotic mycelium mass producing system of claim 1 wherein the spore stock unit is designed to grow a plurality of fruit bodies in sterile conditions for creating the spore stock.
8. A method for producing a mycelium mass, the method comprising the steps of:
providing a mycelium mass producing system having a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit, wherein the mycelium mass producing system comprises a growing space comprising a tray having a conveying platform and a permeable membrane with a plurality of pores positioned on the conveying platform, wherein in a surface of a mass of mycelium grown within said growing space, less than 10% of said surface exhibits the formation of fruiting bodies;
obtaining a live culture sample of fungus to prepare a monokaryon culture at the culture unit;
growing a plurality of fruit bodies in sterile conditions to create a spore stock at the spore stock unit;
performing a peroxide-based spore rescue and a plating process at the plating unit;
sectioning a plurality of robust hyphae upon successful germination of the peroxide-based spore at the section unit;
sub-plating and expanding the plurality of robust hyphae onto a spawn grain master at the sub-plating unit;
subsequently expanding the spawn grain master into production of spawn volume at the expanding unit; and
performing subsequent colonization of a mycelium substrate thereby creating a defect free mass of mycelium at the colonization unit.
9. The method according to claim 8 wherein the environment of the growing space is such that the substrate weight to tray empty space volume ratio is between 0.5 and 5.0 g/cc, the tray empty space volume to substrate volume is between 0.01 and 1.0, and the tray empty space volume to substrate area is between 0.5 and 5.0 cc/cm 2 .
10. The method according to claim 8 wherein the CO 2 concentration within the growing space is held above 3% in steady state conditions, the relative humidity is held above 40% in steady state conditions, and wherein the 02 concentration is held below 20% in steady state conditions to promote mycelium growth without the fruiting body.
11. The method according to claim 8 wherein the defect free mass of mycelium has a surface area wherein less than 5% of said surface area exhibits the formation of fruiting bodies.
12. The method for producing a mycelium mass according to claim 8 wherein the defect free mass of mycelium has a surface area wherein less than 1% of said surface area exhibits the formation of fruiting bodies.
13. The method for producing a mycelium mass according to claim 8 wherein the spore stock unit is designed to grow a plurality of fruit bodies in sterile conditions for creating the spore stock.
14. A method for producing a mycelium mass, the method comprising the steps of:
providing a mycelium mass producing system having a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit;
obtaining a live culture sample of fungus to prepare a monokaryon culture at the culture unit;
growing a plurality of fruit bodies in sterile conditions to create a spore stock at the spore stock unit;
performing a peroxide-based spore rescue and a plating process at the plating unit;
sectioning a plurality of robust hyphae upon successful germination of the peroxide-based spore at the section unit;
sub-plating and expanding the plurality of robust hyphae onto a spawn grain master at the sub-plating unit;
subsequently expanding the spawn grain master into appropriate production of spawn volume at the expanding unit; and
performing subsequent colonization of a mycelium substrate thereby creating a substantially defect free mass of monokaryotic mycelium at the colonization unit.
15. The method according to claim 14 wherein the environment of the growing space is such that the substrate weight to tray empty space volume ratio is between 0.5 and 5.0 g/cc, the tray empty space volume to substrate volume is between 0.01 and 1.0, and the tray empty space volume to substrate area is between 0.5 and 5.0 cc/cm 2 .
16. The method according to claim 14 wherein the CO 2 concentration within the growing space is held above 3% in steady state conditions, the relative humidity is held above 40% in steady state conditions, and wherein the 02 concentration is held below 20% in steady state conditions to promote mycelium growth without the fruiting body.
17. The method for producing a mycelium mass according to claim 14 wherein the defect free mass of mycelium has a surface area wherein less than 5% of said surface area exhibits the formation of fruiting bodies.
18. The method for producing a mycelium mass according to claim 14 wherein the defect free mass of mycelium has a surface area wherein less than 1% of said surface area exhibits the formation of fruiting bodies.
19. The method for producing a mycelium mass according to claim 14 wherein the spore stock unit is designed to grow a plurality of fruit bodies in sterile conditions for creating the spore stock.
20. The method for producing a mycelium mass according to claim 14 wherein the plating unit utilizes the plurality of fruiting bodies for performing the spore rescue and the plating process.