IP Library › Granted Patent US 12,331,199
Granted Patent B2
US 12,331,199 · App. 18/130,212 · Granted Jun 17, 2025

Silicon containing detectable compounds

Inventors: Ronald Graham (Carlsbad, CA); Rodrigo Rodriguez (San Diego, CA)
Assignee: Singular Genomics Systems, Inc.
C09B11/24C07F7/0803C07F7/0825C07F7/083C07H21/02C07H21/04C09B69/008C07F7/0816C07H23/00
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Quick Facts
Patent No.
US 12,331,199
App. No.
18/130,212
Granted
Jun 17, 2025
Kind
B2
Abstract

Disclosed herein, inter alia, are silicon containing detectable compounds and methods of use thereof.

Claims (87)

1. A compound, or salt thereof, having the formula:

wherein,

L 3 is

L 1 -L 2 -comprises

R 1 is substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;

R 2 and R 3 are independently substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;

R 4 is hydrogen, R 34 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 34 -substituted or unsubstituted heteroalkyl, R 34 -substituted or unsubstituted cycloalkyl, or R 34 -substituted or unsubstituted heterocycloalkyl;

R 5 is hydrogen, R 35 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 35 -substituted or unsubstituted heteroalkyl, R 35 -substituted or unsubstituted cycloalkyl, or R 35 -substituted or unsubstituted heterocycloalkyl;

R 6 is hydrogen, R 36 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 36 -substituted or unsubstituted heteroalkyl, R 36 -substituted or unsubstituted cycloalkyl, or R 36 -substituted or unsubstituted heterocycloalkyl;

R 7 is hydrogen, R 37 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 37 -substituted or unsubstituted heteroalkyl, R 37 -substituted or unsubstituted cycloalkyl, or R 37 -substituted or unsubstituted heterocycloalkyl;

R 8 is a nucleotide;

R 9 is hydrogen, R 39 -substituted or unsubstituted alkyl, or R 39 -substituted or unsubstituted heteroalkyl;

R 10 is hydrogen, R 40 -substituted or unsubstituted alkyl, or R 40 -substituted or unsubstituted heteroalkyl;

R 11 is hydrogen, R 41 -substituted or unsubstituted alkyl, or R 41 -substituted or unsubstituted heteroalkyl;

R 12 is hydrogen, R 42 -substituted or unsubstituted alkyl, or R 42 -substituted or unsubstituted heteroalkyl;

R 4 and R 5 substituents bonded to the same nitrogen atom may optionally be joined to form a R 34 -substituted or unsubstituted heterocycloalkyl;

R 6 and R 7 substituents bonded to the same nitrogen atom may optionally be joined to form a R 36 -substituted or unsubstituted heterocycloalkyl;

R 4 and R 9 substituents may optionally be joined to form a R 34 -substituted or unsubstituted heterocycloalkyl, or R 39 -substituted or unsubstituted heteroaryl;

R 7 and R 10 substituents may optionally be joined to form a R 37 -substituted or unsubstituted heterocycloalkyl, or R 37 -substituted or unsubstituted heteroaryl;

R 5 and R 11 substituents may optionally be joined to form a R 35 -substituted or unsubstituted heterocycloalkyl, or R 35 -substituted or unsubstituted heteroaryl;

R 6 and R 12 substituents may optionally be joined to form a R 36 -substituted or unsubstituted heterocycloalkyl, or R 36 -substituted or unsubstituted heteroaryl;

R 34 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 35 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 36 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 37 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 39 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H;

R 40 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H;

R 41 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H; and

R 42 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H.

2. The compound of claim 1 , wherein R 1 is substituted or unsubstituted C 1 -C 6 alkyl.

3. The compound of claim 1 , wherein R 2 and R 3 are independently substituted or unsubstituted C 1 -C 6 alkyl, or substituted or unsubstituted 2 to 6 membered heteroalkyl.

4. The compound of claim 1 , wherein R 4 is hydrogen, R 34 -substituted or unsubstituted C 1 -C 6 alkyl, or R 34 -substituted or unsubstituted 2 to 6 membered heteroalkyl;

R 5 is hydrogen, R 35 -substituted or unsubstituted C 1 -C 6 alkyl, or R 35 -substituted or unsubstituted 2 to 6 membered heteroalkyl;

R 6 is hydrogen, R 36 -substituted or unsubstituted C 1 -C 6 alkyl, or R 36 -substituted or unsubstituted 2 to 6 membered heteroalkyl; and

R 7 is hydrogen, R 37 -substituted or unsubstituted C 1 -C 6 alkyl, or R 37 -substituted or unsubstituted 2 to 6 membered heteroalkyl.

5. The compound of claim 1 , wherein R 4 and R 5 substituents bonded to the same nitrogen atom are joined to form a R 34 -substituted or unsubstituted 5 membered heterocycloalkyl or R 34 -substituted or unsubstituted 6 membered heterocycloalkyl.

6. The compound of claim 1 , wherein R 6 and R 7 substituents bonded to the same nitrogen atom are joined to form a R 36 -substituted or unsubstituted 5 membered heterocycloalkyl or R 36 -substituted or unsubstituted 6 membered heterocycloalkyl.

7. The compound of claim 1 , wherein R 8 is

wherein

B is a divalent base;

R 15 is a 5′-nucleoside protecting group, monophosphate moiety, polyphosphate moiety, or nucleic acid moiety;

R 16 is hydrogen or —OH; and

R 17 is an —O-polymerase-compatible cleavable moiety, wherein said polymerase-compatible cleavable moiety has the formula:

8. The compound of claim 7 , wherein R 15 is a triphosphate moiety.

9. The compound of claim 7 , wherein

B is a divalent cytosine, divalent guanine, divalent adenine, divalent thymine, divalent uracil, divalent hypoxanthine, divalent xanthine, divalent 7-methylguanine, divalent 5,6-dihydrouracil, divalent 5-methylcytosine, or divalent 5-hydroxymethylcytosine.

10. A method of detecting the presence of an agent, said method comprising exposing a compound covalently bound to said agent to absorption light and detecting an emission wavelength, wherein said agent is an oligonucleotide, protein, or compound, and wherein said compound covalently bound to said agent has the formula:

wherein,

L 3 is

L 1 -L 2 -comprises

R 1 is substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;

R 2 and R 3 are independently substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;

R 4 is hydrogen, R 34 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 34 -substituted or unsubstituted heteroalkyl, R 34 -substituted or unsubstituted cycloalkyl, R 34 -substituted or unsubstituted heterocycloalkyl, R 34 -substituted or unsubstituted aryl, or R 34 -substituted or unsubstituted heteroaryl;

R 5 is hydrogen, R 35 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 35 -substituted or unsubstituted heteroalkyl, R 35 -substituted or unsubstituted cycloalkyl, R 35 -substituted or unsubstituted heterocycloalkyl, R 35 -substituted or unsubstituted aryl, or R 35 -substituted or unsubstituted heteroaryl;

R 6 is hydrogen, R 36 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 36 -substituted or unsubstituted heteroalkyl, R 36 -substituted or unsubstituted cycloalkyl, R 36 -substituted or unsubstituted heterocycloalkyl, R 36 -substituted or unsubstituted aryl, or R 36 -substituted or unsubstituted heteroaryl;

R 7 is hydrogen, R 37 -substituted or unsubstituted alkyl, halogen, —CF 3 , —CBr 3 , —CCl 3 , —CI 3 , —CHF 2 , —CHBr 2 , —CHCl 2 , —CHI 2 , —CH 2 F, —CH 2 Br, —CH 2 Cl, —CH 2 I, —OH, —NH 2 , —COOH, —CONH 2 , —SH, R 37 -substituted or unsubstituted heteroalkyl, R 37 -substituted or unsubstituted cycloalkyl, R 37 -substituted or unsubstituted heterocycloalkyl, R 37 -substituted or unsubstituted aryl, or R 37 -substituted or unsubstituted heteroaryl;

R 9 is hydrogen, R 39 -substituted or unsubstituted alkyl, or R 39 -substituted or unsubstituted heteroalkyl;

R 10 is hydrogen, R 40 -substituted or unsubstituted alkyl, or R 40 -substituted or unsubstituted heteroalkyl;

R 11 is hydrogen, R 41 -substituted or unsubstituted alkyl, or R 41 -substituted or unsubstituted heteroalkyl;

R 12 is hydrogen, R 42 -substituted or unsubstituted alkyl, or R 42 -substituted or unsubstituted heteroalkyl;

R 4 and R 5 substituents bonded to the same nitrogen atom may optionally be joined to form a R 34 -substituted or unsubstituted heterocycloalkyl;

R 6 and R 7 substituents bonded to the same nitrogen atom may optionally be joined to form a R 36 -substituted or unsubstituted heterocycloalkyl;

R 4 and R 9 substituents may optionally be joined to form a R 34 -substituted or unsubstituted heterocycloalkyl, or R 39 -substituted or unsubstituted heteroaryl;

R 7 and R 10 substituents may optionally be joined to form a R 37 -substituted or unsubstituted heterocycloalkyl, or R 37 -substituted or unsubstituted heteroaryl;

R 5 and R 11 substituents may optionally be joined to form a R 35 -substituted or unsubstituted heterocycloalkyl, or R 35 -substituted or unsubstituted heteroaryl;

R 6 and R 12 substituents may optionally be joined to form a R 36 -substituted or unsubstituted heterocycloalkyl, or R 36 -substituted or unsubstituted heteroaryl;

R 34 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 35 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 36 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 37 is independently oxo, halogen, —CF 3 , —CCl 3 , —CI 3 , —CBr 3 , —CHF 2 , —CHCl 2 , —CHI 2 , —CHBr 2 , —CH 2 F, —CH 2 Cl, —CH 2 I, —CH 2 Br, —OCH 2 F, —OCH 2 Cl, —OCH 2 I, —OCH 2 Br, —OCHF 2 , —OCHCl 2 , —OCHI 2 , —OCHBr 2 , —OCF 3 , —OCCl 3 , —OCI 3 , —OCBr 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC═(O)NHNH 2 , —NHC═(O)NH 2 , —NHSO 2 H, —NHC═(O)H, —NHC(O)OH, —NHOH, —PO 4 H, —PO 3 H, —N 3 , unsubstituted alkyl, unsubstituted heteroalkyl, unsubstituted cycloalkyl, or unsubstituted heterocycloalkyl;

R 39 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H;

R 40 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H;

R 41 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H; and

R 42 is independently —PO 3 H, —PO 4 H, —SO 2 NH 2 , —SO 3 H, or —SO 4 H.

11. The compound of claim 1 having formula:

wherein, the symbol B is a divalent base; and

the symbol in formula (XI) or (XII) refers to an attachment point to hydrogen, a phosphate moiety, or a reversible terminator moiety.

12. The compound of claim 1 , wherein R 4 and R 9 substituents are joined to form a R 34 -substituted or unsubstituted 7 membered heterocycloalkyl, or R 39 -substituted or unsubstituted 7 membered heteroaryl.

13. The compound of claim 1 , wherein R 7 and R 10 substituents are joined to form a R 37 -substituted or unsubstituted 7 membered heterocycloalkyl, or R 37 -substituted or unsubstituted 7 membered heteroaryl.

14. The compound of claim 1 , wherein R 5 and R 11 substituents are joined to form a R 35 -substituted or unsubstituted 6 membered heterocycloalkyl, or R 35 -substituted or unsubstituted 6 membered heteroaryl.

15. The compound of claim 1 , wherein R 6 and R 12 substituents are joined to form a R 36 -substituted or unsubstituted 6 membered heterocycloalkyl, or R 36 -substituted or unsubstituted 6 membered heteroaryl.

16. The compound of claim 1 , wherein

R 4 and R 9 substituents are joined to form a R 34 -substituted or unsubstituted 6 membered heterocycloalkyl, or R 39 -substituted or unsubstituted 6 membered heteroaryl;

R 7 and R 10 substituents are joined to form a R 37 -substituted or unsubstituted 6 membered heterocycloalkyl, or R 37 -substituted or unsubstituted 6 membered heteroaryl;

R 5 and R 11 substituents are joined to form a R 35 -substituted or unsubstituted 6 membered heterocycloalkyl, or R 35 -substituted or unsubstituted 6 membered heteroaryl; and

R 6 and R 12 substituents are joined to form a R 36 -substituted or unsubstituted 6 membered heterocycloalkyl, or R 36 -substituted or unsubstituted 6 membered heteroaryl.

17. The compound of claim 1 , having formula

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 17, 2025
From: GRAHAM, RONALD; RODRIGUEZ, RODRIGO
To: SINGULAR GENOMICS SYSTEMS, INC.
Reel/Frame 070876/0076 →
SECURITY INTEREST Recorded Mar 7, 2025
From: SINGULAR GENOMICS SYSTEMS, INC.
To: FIRST-CITIZENS BANK & TRUST COMPANY
Reel/Frame 070440/0465 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 2, 2023
From: GRAHAM, RONALD; RODRIGUEZ, RODRIGO
To: SINGULAR GENOMICS SYSTEMS, INC
Reel/Frame 063505/0042 →
Continuity (3)
Continuation 16415700 · May 17, 2019
Provisional Application 62673579 · May 18, 2018
Related Publication 20230295437A1 · Sep 21, 2023
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