DNA methylation and mutational analysis methods for bladder cancer surveillance
The present disclosure relates to methods of monitoring bladder cancer patients and analyzing patient samples for presence of methylated DNA and optionally particular gene mutations. In some embodiments, analysis results are correlated with clinical outcome measures such as risk of bladder cancer recurrence.
1 . A method of characterizing a urine sediment sample obtained from a subject, comprising:
bisulfite converting DNA from the sample; and
performing methylation-specific quantitative PCR on the bisulfite converted DNA to determine the amount and/or the concentration of methylated DNA in the sample from a set of genes consisting of at least four genes selected from MEIS1, NKPD1, ONECUT2, KLF2, OSR1, SOX1, EOMES, DDX25, TMEM106A, EPHX3, IRX5, NID2, VIM, and ITPKB, wherein the set of genes comprises one or both of NKPD1 and KLF2, and from a set of one or more reference genes, wherein the reference gene(s) have a methylation level that is not significantly different in a subject with bladder cancer compared to a subject without bladder cancer.
2 . The method of claim 1 , wherein the subject has or is suspected of having bladder cancer.
3 . The method of claim 2 , wherein the subject has been diagnosed with non-muscle-invasive bladder cancer.
4 . The method of claim 2 , wherein the sample is obtained from the subject prior to cystoscopy or after a negative cystoscopy.
5 . The method of claim 1 , wherein the set of genes comprises both of NKPD1 and KLF2.
6 . The method of claim 1 , wherein the set of one or more reference genes comprises one or more of CTNS, TOP3A, COL2A and SLC24A3.
7 . The method of claim 1 , wherein the set of genes consists of one of the following gene sets:
(a) MEIS1, NKPD1, ONECUT2, KLF2;
(b) MEIS1, NKPD1, ONECUT2, OSR1;
(c) MEIS1, NKPD1, KLF2, SOX1;
(d) MEIS1, NKPD1, ONECUT2, OSR1, TMEM106A; EPHX3;
(e) MEIS1, NKPD1, ONECUT2, KLF2, TMEM106A, IRX5;
(f) MEIS1, NKPD1, ONECUT2, KLF2, SOX1, TMEM106A; or
(g) MEIS1, NKPD1, ONECUT2, KLF2, TMEM106A, ITPKB.
8 . The method of claim 1 , wherein the method further comprises analyzing the bisulfite converted DNA for mutations in one or both of FGFR3 and TERT.
9 . The method of claim 1 , wherein the set of genes consists of four genes wherein the four genes comprise MEIS1, NKPF1, and KLF2.
10 . A method of characterizing a urine sediment sample obtained from a subject, comprising:
bisulfite converting DNA from the sample; and
performing methylation-specific quantitative PCR on the bisulfite converted DNA to determine the amount and/or the concentration of methylated DNA in the sample from a set of genes consisting of three or four genes, wherein the set of three or four genes comprises one or both of NKPD1 and KLF2; and from a set of one or more reference genes wherein the reference gene(s) have a methylation level that is not significantly different in a subject with bladder cancer compared to a subject without bladder cancer.
11 . The method of claim 10 , wherein the subject has or is suspected of having bladder cancer.
12 . The method of claim 11 , wherein the subject has been diagnosed with non-muscle-invasive bladder cancer.
13 . The method of claim 11 , wherein the sample is obtained from the patient prior to cystoscopy or after a negative cystoscopy.
14 . The method of claim 10 , wherein the set of three or four genes consists of (a) one or both of NKPD1 and KLF2 and (b) one, two, or three genes selected from MEIS1, ONECUT2, OSR1, SOX1, EOMES, DDX25, and TMEM106A.
15 . The method of claim 10 , wherein the set of three or four genes comprises both of NKPD1 and KLF2.
16 . The method of claim 10 , wherein the set of three or four genes consists of MEIS1, NKPD1, ONECUT2, and KLF2.
17 . The method of claim 10 , wherein the method further comprises analyzing the bisulfite converted DNA for mutations in one or both of FGFR3 and TERT.
18 . The method of claim 10 , wherein the set of one or more reference genes comprises one or more of CTNS, TOP3A, COL2A and SLC24A3.
19 . The method of claim 10 , wherein the set of three or four genes consists of MEIS1, NKPD1, and KLF2.