RSV F protein compositions and methods for making same
The present invention relates to immunogenic compositions comprising RSV F protein, methods for preparing compositions that contain RSV F protein ecto-domain polypeptides, and to certain engineered RSV F proteins and nucleic acids that encode the engineered RSV F proteins. Compositions prepared using the methods can contain RSV F protein ecto-domain polypeptides in a predominant or single desired form and conformation. The invention also relates to methods for inducing an immune response to RSV F.
1. A protein comprising: i) a respiratory syncytial virus F (RSV F) ectodomain of a RSV A subgroup; and ii) a trimerizing sequence from bacteriophage T4 fibritin comprising amino acid residues 3-29 of SEQ ID NO: 19.
2. The protein of claim 1 , wherein the protein does not comprise a transmembrane region or cytoplasmic tail.
3. The protein of claim 1 , wherein the protein does not comprise a p27 region.
4. A nucleic acid encoding the protein of claim 1 .
5. A vector comprising the nucleic acid of claim 4 .
6. A host cell comprising the vector of claim 5 .
7. A method of making a protein comprising: i) a respiratory syncytial virus F (RSV F) ectodomain of a RSV A subgroup; and ii) a trimerizing sequence from bacteriophage T4 fibritin comprising amino acid residues 3-29 of SEQ ID NO: 19, the method comprising culturing the host cell of claim 6 and recovering the protein from a culture media.
8. An immunogenic composition comprising the protein of claim 1 .
9. The immunogenic composition of claim 8 , further comprising an adjuvant.
10. A method of inducing an immune response to RSV in a subject, the method comprising administering an effective amount of the immunogenic composition of claim 8 to the subject.
11. A protein comprising: i) a respiratory syncytial virus F (RSV F) ectodomain of a RSV B subgroup; and ii) a trimerizing sequence from bacteriophage T4 fibritin comprising amino acid residues 3-29 of SEQ ID NO: 19.
12. The protein of claim 11 , wherein the protein does not comprise a transmembrane region or cytoplasmic tail.
13. The protein of claim 11 , wherein the protein does not comprise a p27 region.
14. A nucleic acid encoding the protein of claim 11 .
15. A vector comprising the nucleic acid of claim 14 .
16. A host cell comprising the vector of claim 15 .
17. A method of making a protein comprising: i) a respiratory syncytial virus F (RSV F) ectodomain of a RSV B subgroup; and ii) a trimerizing sequence from bacteriophage T4 fibritin comprising amino acid residues 3-29 of SEQ ID NO: 19, the method comprising culturing the host cell of claim 16 and recovering the protein from a culture media.
18. An immunogenic composition comprising the protein of claim 11 .
19. The immunogenic composition of claim 18 , further comprising an adjuvant.
20. A method of inducing an immune response to RSV in a subject, the method comprising administering an effective amount of the immunogenic composition of claim 18 to the subject.