Chemical compositions and methods of using same
The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays.
1. A probe comprising a target binding domain linked to a barcode domain;
wherein the target binding domain comprises at least eight nucleotides and is capable of binding a target nucleic acid, wherein at least six nucleotides in the target binding domain are capable of identifying a target nucleic acid molecule and wherein at least two nucleotides in the target binding domain do not identify the target nucleic acid molecule;
wherein the barcode domain comprises at least one attachment position, each attachment position comprising at least one attachment region comprising at least one nucleic acid sequence capable of being bound by a complementary nucleic acid molecule, wherein the nucleic acid sequence of the at least one attachment position determines the identity of the target nucleic acid that is bound by the target binding domain.
2. The probe of claim 1 , wherein said barcode domain comprises a single-stranded DNA.
3. The probe of claim 1 , wherein each attachment position in the barcode domain comprises one attachment region.
4. The probe of claim 1 , wherein the at least two nucleotides in the target binding domain that do not identify the target nucleic acid molecule follow the at least six nucleotides in the target binding domain that identify the target nucleic acid molecule.
5. The probe of claim 1 , wherein the at least six nucleotides in the target binding domain that are capable of identifying the target nucleic acid molecule are complementary to a portion of the target nucleic acid molecule.
6. The probe of claim 1 , wherein the at least two nucleotides in the target binding domain that are not capable of identifying a target nucleic acid molecule are not complementary to a portion of the target nucleic acid molecule.
7. The probe of claim 1 , wherein the barcode domain comprises one attachment position.
8. The probe of claim 1 , wherein each attachment position comprises between 8 nucleotides and 20 nucleotides.
9. The probe of claim 1 , wherein each attachment position comprises about 20 nucleotides.
10. The probe of claim 1 , further comprising: a complementary nucleic acid molecule hybridized to a first attachment region of a first attachment position of the barcode domain.
11. The probe of claim 10 , wherein the complementary nucleic acid molecule comprises at least one detectable label.
12. The probe of claim 11 , wherein the at least one detectable label is a fluorescent label.
13. A population of probes comprising a plurality of the probe of claim 1 .
14. The probe of claim 1 , wherein at least eight nucleotides in the target binding domain are capable of identifying the target nucleic acid molecule.
15. The probe of claim 1 , wherein the target binding domain comprises at least ten nucleotides.
16. The probe of claim 15 , wherein at least ten nucleotides in the target binding domain are capable of identifying the target nucleic acid molecule.