IP Library › Granted Patent US 12,258,606
Granted Patent B2
US 12,258,606 · App. 18/183,669 · Granted Mar 25, 2025

Limiting yeast-produced trehalose in fermentation

Inventors: Charles F. Rice (Plainfield, NH); Ryan Skinner (Bethel, VT); Trisha Barrett (Bradford, VT); Aaron Argyros (Lebanan, NH)
Assignee: DANSTAR FERMENT AG
C12P7/10C12N1/18C12N9/2402C12N15/81C12P7/20C12Y302/01028C12P19/12Y02E50/10
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Quick Facts
Patent No.
US 12,258,606
App. No.
18/183,669
Granted
Mar 25, 2025
Kind
B2
Abstract

The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).

Claims (17)

1. A method of increasing the production of a fermentation product during a fermentation, said method comprising contacting a medium with (i) a recombinant yeast host cell comprising a first genetic modification for expressing a heterologous glucose-inactivated glycerol proton symporter STL1 (STL1) protein and (ii) a trehalase, wherein the production of the fermentation product is increased when compared to a control method with a control yeast host cell lacking the first genetic modification.

2. The method of claim 1 , wherein the recombinant yeast host cell comprises a further genetic modification for reducing the production of one or more native enzymes that function to produce glycerol.

3. The method of claim 2 , wherein the recombinant yeast host cell expresses less of a native glycerol-3-phosphate dehydrogenase 1 (GPD1) polypeptide than a control yeast host cell lacking the further genetic modification.

4. The method of claim 2 , wherein the recombinant yeast host cell lacks the ability to produce a native GPD1 polypeptide.

5. The method of claim 2 , wherein the recombinant yeast host cell expresses less of a native glycerol-3-phosphate dehydrogenase 2 (GPD2) polypeptide than a control yeast host cell lacking the further genetic modification.

6. The method of claim 2 , wherein the recombinant yeast host cell lacks the ability to produce a native GPD2 polypeptide.

7. The method of claim 1 , wherein the recombinant yeast host cell is from the genus Saccharomyces sp.

8. The method of claim 7 , wherein the recombinant yeast host cell is from the species Saccharomyces cerevisiae.

9. The method of claim 1 , wherein the trehalase is extracellular from the recombinant yeast host cell.

10. The method of claim 1 , wherein the trehalase is provided in a purified form.

11. The method of claim 1 , wherein the trehalase belongs to E.C. 3.1.2.1.28.

12. The method of claim 1 , wherein the trehalase is an acid trehalase.

13. The method of claim 1 , wherein the trehalase is a neutral trehalase.

14. The method of claim 1 , wherein the medium comprises trehalose.

15. The method of claim 1 , wherein the medium comprises starch.

16. The method of claim 15 , wherein the starch is provided in a gelatinized or a raw form.

17. The method of claim 1 , wherein the medium is derived from corn.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 26, 2024
From: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC
To: DANSTAR FERMENT AG
Reel/Frame 068174/0430 →
Continuity (5)
Continuation 17170529 · Feb 8, 2021
Continuation 16776389 · Jan 29, 2020
Continuation 15773139
Provisional Application 62251885 · Nov 6, 2015
Related Publication 20230265463A1 · Aug 24, 2023
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