USE OF ANTI-IL-27 ANTIBODIES
The present disclosure relates to methods of treating cancer comprising administering to a subject an anti-IL-27 antibody and pembrolizumab.
1 . A method of stimulating an immune response in a subject, the method comprising administering to the subject (i) an antibody that binds human IL-27 or an antigen binding portion thereof (“an anti-IL-27 antibody”), and (ii) pembrolizumab; wherein the anti-IL-27 antibody comprises a heavy chain CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 5, a heavy chain CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 6, a heavy chain CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 7, a light chain CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 13, a light chain CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 14, and a light chain CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 15.
2 . A method of treating a cancer in a subject in need thereof comprising administering to the subject (i) an antibody that binds human IL-27 or an antigen binding portion thereof (“an anti-IL-27 antibody”), and (ii) pembrolizumab; wherein the anti-IL-27 antibody comprises a heavy chain CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 5, a heavy chain CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 6, a heavy chain CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 7, a light chain CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 13, a light chain CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 14, and a light chain CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 15.
3 . The method of claim 2 , wherein the anti-IL-27 antibody is administered at a dose of at least about 0.003 mg/kg to at least about 20 mg/kg.
4 . (canceled)
5 . The method of claim 2 , wherein the anti-IL-27 antibody is administered once about every week, once about every two weeks, once about every three weeks, once about every four weeks, once about every 6 weeks, once about every 8 weeks, or once about every 12 weeks.
6 - 18 . (canceled)
19 . The method of claim 2 , wherein pembrolizumab is administered at a dose of at least about 1 mg/kg to at least about 10 mg/kg.
20 . (canceled)
21 . The method of claim 2 , wherein pembrolizumab is administered at a flat dose.
22 . (canceled)
23 . The method of claim 2 , wherein pembrolizumab is administered once about every week, once about every two weeks, once about every three weeks, or once about every four weeks.
24 - 34 . (canceled)
35 . The method of claim 2 , wherein the anti-IL-27 antibody and pembrolizumab are administered on the same day.
36 . The method of claim 2 , wherein the anti-IL-27 antibody and pembrolizumab are administered concurrently.
37 . The method of claim 2 , wherein the anti-IL-27 antibody and pembrolizumab are administered sequentially.
38 . The method of claim 2 , wherein the anti-IL-27 antibody is administered before pembrolizumab.
39 . The method of claim 2 , wherein the anti-IL-27 antibody is administered after pembrolizumab.
40 . The method of claim 2 , wherein the anti-IL-27 antibody and pembrolizumab are administered on different days.
41 . The method of claim 2 , wherein the anti-IL-27 antibody comprises a heavy chain variable region comprising an amino acid sequence that has at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, [[or]] at least about 99%, or at least 100% sequence identity to the amino acid sequence set forth in SEQ ID NO: 11.
42 . (canceled)
43 . The method of claim 2 , wherein the anti-IL-27 antibody comprises a light chain variable region comprising an amino acid sequence that has at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least 100% sequence identity to the amino acid sequence set forth in SEQ ID NO: 19.
44 . (canceled)
45 . (canceled)
46 . The method of claim 2 , wherein the anti-IL-27 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 21.
47 . The method of claim 2 , wherein the anti-IL-27 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 25.
48 . The method of claim 2 , wherein the anti-IL-27 antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO: 23.
49 . (canceled)
50 . (canceled)
51 . The method of claim 1 , wherein the subject is afflicted with a cancer.
52 - 54 . (canceled)
55 . The method of claim 2 , wherein the subject previously received at least one, at least two, at least three, at least four, or at least five prior anticancer therapies.
56 - 118 . (canceled)