Compositions and methods for treating intervertebral discs
A biocompatible antimicrobial composition effective against microbial biofilms, including in vivo biofilms containing gram-positive bacteria, can be delivered to and used near the spinal column of vertebrates. Once introduced to an area colonized with a bacterial biofilm, particularly an intervertebral disc, the antimicrobial composition weakens the EPS/ECPS macromolecules of the biofilm, which makes the entire biofilm structure susceptible to being dislodged from the affected area or biosorbed. Additionally or alternatively, certain of the subcomponents of the solute component, as well as perhaps the organic liquid(s) of the solvent component, might lyse microbes, particularly gram-positive bacteria, previously protected by the EPS/ECPS macromolecules. Swelling of and around previously impacted intervertebral discs can be reduced or eliminated, often resulting in appreciable reductions in patient pain levels.
1. A method for treating a spinal column area colonized with a biofilm that comprises at least one type of gram-positive bacterium which includes Propionibacterium acnes , said area being an intervertebral disc or tissue adjacent thereto, said method comprising introducing to said area, via injection, a liquid composition effective to weaken the macromolecules of said biofilm and, optionally, lyse the cell walls of one or more types of bacteria contained therein, said liquid composition having an effective solute concentration of 0.1 to 1.1 Osm/L, wherein said liquid composition comprises a solvent component and a solute component, wherein said solute component comprises a buffer precursor that comprises at least one weak acid and at least one salt of said at least one weak acid.
2. The method of claim 1 wherein said liquid has a dynamic viscosity of no more than about 125 mPa·s.
3. The method of claim 2 wherein said liquid increases in viscosity after said introduction.
4. The method of claim 3 wherein said liquid solidifies after said introduction.
5. The method of claim 1 wherein said liquid increases in viscosity after said introduction.
6. The method of claim 5 wherein said liquid solidifies after said introduction.
7. The method of claim 5 wherein said liquid composition has an effective solute concentration of no more than 600 mOsm/L.
8. The method of claim 1 wherein the lower limit of said effective solute concentration is 200 mOsm/L.
9. The method of claim 1 wherein the upper limit of said effective solute concentration is 600 mOsm/L.
10. The method of claim 1 wherein said liquid composition has a pH of from 5.0 to 6.5.
11. The method of claim 10 wherein said liquid composition consists of the solvent component and the solute component.
12. The method of claim 1 wherein said buffer precursor comprises citric acid and a salt of citric acid.
13. The method of claim 12 wherein said solute component further comprises at least one of salicylic acid and less than 1% (w/v) surfactant.
14. The method of claim 1 wherein said solvent component consists of water and at least one organic liquid.
15. The method of claim 14 wherein said solvent component exhibits a δ p value of less than 15.2 MPa 1/2 .
16. The method of claim 1 wherein said solute component further comprises less than 1% (w/v) surfactant.
17. A method for treating a spinal column area colonized with a biofilm that comprises Propionibacterium acnes , said method comprising introducing to an intervertebral disc or tissue adjacent thereto, via injection, a liquid composition effective to weaken the macromolecules of said biofilm and, optionally, lyse the cell walls of said at least one type of gram-positive bacterium contained therein, said liquid composition having an effective solute concentration of 0.1 to 1.1 Osm/L and a pH of from 5.0 to 6.5, said liquid composition comprising a solvent component and a solute component, wherein said solute component comprises a buffer precursor that comprises at least one weak acid and at least one salt of said at least one weak acid and wherein said solvent component exhibits a δ p value of less than 15.2 MPa 1/2 and consists of water and at least one organic liquid.