IP Library Granted Patent US 12,188,716
Granted Patent B2
US 12,188,716 · App. 18/231,440 · Granted Jan 7, 2025

Method and apparatus for freezing dispensed droplets of liquid

Inventor: Reza Bashar (San Diego, CA)
Assignee: GEN-PROBE INCORPORATED
F25D3/11A01N1/0252B01J2/06
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Quick Facts
Patent No.
US 12,188,716
App. No.
18/231,440
Granted
Jan 7, 2025
Kind
B2
Abstract

A method and apparatus for freezing a liquid droplet includes dispensing, by a liquid dispenser, a droplet of liquid into a fluid chamber containing a freezing liquid. The droplet of liquid is allowed to dwell in the freezing liquid for at least a predetermined dwell time so that the droplet of liquid freezes to a frozen droplet. The method and apparatus further includes injecting, by a gas injector, a stream of gas transversely to a surface of the freezing liquid at about where the frozen droplet is located along the surface of the freezing liquid contained in the fluid chamber so that the frozen droplet sinks in the freezing liquid.

Claims (9)

1. An apparatus comprising: a plurality of fluid chambers, each containing a freezing liquid; a transporter configured to move the plurality of fluid chambers about an axis of rotation, wherein the plurality of fluid chambers comprises two or more groups of fluid chambers, each group of fluid chambers being located at a different corresponding radial distance from the axis of rotation; two or more liquid dispensers disposed above a surface of the freezing liquid contained in the plurality of fluid chambers, wherein each of the two or more liquid dispensers is located at the corresponding radial distance from the axis of rotation at which a different one of the groups of fluid chambers is located, and wherein each liquid dispenser is configured to dispense a droplet of liquid onto the freezing liquid contained in one of the plurality of fluid chambers of the group of fluid chambers located at the corresponding radial distance; and two or more gas injectors disposed above the surface of the freezing liquid contained in the plurality of fluid chambers, wherein each of the two or more gas injectors is located at the corresponding radial distance from the axis of rotation at which a different one of the groups of fluid chambers is located, and wherein each gas injector of the two or more gas injectors is configured to inject a stream of gas transversely to the surface of the freezing liquid contained in one of the plurality of fluid chambers of the group of fluid chambers located at the corresponding radial distance, wherein movement of the plurality of chambers about the axis of rotation by the transporter sequentially positions different fluid chambers of each group of fluid chambers below the liquid dispenser located at the corresponding radial distance and sequentially positions the different fluid chambers of each of the group of fluid chambers below each of the gas injector of the two or more gas injectors located at the corresponding radial distance, wherein the fluid chamber positioned below the liquid dispenser located at each of the corresponding radial distances is different from the fluid chamber positioned below each of the gas injector of the two or more gas injectors at the same corresponding radial distance.

2. The apparatus of claim 1 , wherein the transporter comprises a carousel configured to move the plurality of fluid chambers about the axis of rotation.

3. The apparatus of claim 2 , wherein the carousel comprises a drum and the plurality of fluid chambers are disposed within the drum.

4. The apparatus of claim 3 , wherein the carousel comprises a spindle configured to rotate about the axis of rotation, and the spindle is coupled to the drum such that the drum is configured to rotate with the spindle about the axis of rotation.

5. The apparatus of claim 3 , wherein the carousel comprises a lid coupled to a top end of the drum, wherein the plurality of fluid chambers are disposed beneath the lid, and the lid comprises a plurality of openings, and each opening is aligned with one of the plurality of fluid chambers the fluid chamber.

6. The apparatus of claim 3 , wherein the carousel comprises an insulation layer disposed between the drum and the plurality of fluid chambers.

7. The apparatus of claim 3 , wherein the carousel comprises a strainer basket disposed in the drum, and the strainer basket is configured to receive and hold a frozen droplet sinking through the freezing liquid contained in the plurality of fluid chambers toward a bottom of the drum.

8. The apparatus of claim 1 , wherein each of the gas injector of the two or more gas injectors comprises a nozzle, and wherein the nozzle is configured to inject the gas stream transversely to the surface of the freezing liquid contained in each fluid chamber located at the corresponding radial distance, and wherein the apparatus further comprises: a solenoid valve controlling gas flow to the nozzle and configured to switch between a closed position to shut-off the gas stream from reaching the nozzle and an open position to permit the gas stream to reach the nozzle; a sensor configured to generate a signal relating to a position of the transporter; and a control unit in electrical communication with the sensor and the solenoid valve, wherein the control unit is configured to receive the signal from the sensor and transmit a command to the solenoid valve to switch between the open and closed positions based on the signal.

9. The apparatus of claim 1 , wherein the transporter is configured to transport each fluid chamber from a first position below the liquid dispenser located at the corresponding radial distance to a second position below each of the gas injector of the two or more gas injectors located at the corresponding radial distance at a predetermined dwell time so that the droplet of liquid dispensed by the liquid dispenser freezes to a frozen droplet before each of the gas injector of the two or more gas injectors injects the stream of gas transversely to the surface of the freezing liquid contained in the fluid chamber.

Assignments (2)
SECURITY INTEREST Recorded Apr 8, 2026
From: BIOTHERANOSTICS, INC.; GEN-PROBE INCORPORATED; GEN-PROBE PRODESSE, INC.; CYTYC CORPORATION; SUROS SURGICAL SYSTEMS, INC.; GYNESONICS, INC.; BOLDER SURGICAL, LLC; FAXITRON BIOPTICS, LLC; HEALTH BEACONS, INC.; HOLOGIC, INC.
To: ROYAL BANK OF CANADA, AS COLLATERAL AGENT
Reel/Frame 075462/0440 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 20, 2023
From: BASHAR, REZA
To: GEN-PROBE INCORPORATED
Reel/Frame 064965/0624 →
Continuity (3)
Continuation 15734935
Provisional Application 62687562 · Jun 20, 2018
Related Publication 20230392850A1 · Dec 7, 2023
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