Granted Patent B2

US 12,282,015 · App. 18/243,045 · Granted Apr 22, 2025

Inventors: Anne North (Pleasant Hill, CA); Naheed Mufti (Concord, CA)

Assignee: Cerus Corporation

G01N33/502G01N33/555

Abstract

Claims (17)

1. A method of administering a red blood cell transfusion to a patient, wherein the red blood cell transfusion comprises red blood cells treated with a pathogen-inactivating compound β-alanine, N-(acridin-9-yl), 2-[bis(2-chloroethyl)amino]ethyl ester (S-303-treated RBCs), the method comprising:

a) testing a sample from the patient for presence of antibodies reactive to the S-303-treated RBCs according to a method comprising:

i) providing the sample from the patient, wherein the sample comprises serum or plasma;

ii) contacting the sample with a first substrate, wherein a moiety is bound to the surface of the first substrate, and wherein the surface-bound moiety resulted from treating the first substrate with a compound of Formula VIII:

where R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , and R 8 are each —H; R 20 is —H; and R 21 is —R 11 —X-E, where —R 11 — is unsubstituted-C 1-8 alkyl-; X is independently —R 11 —; and E is N(R 12 ) 2 ; where —R 12 is —CH 2 CH 2 -G, and where each G is independently —Cl, —Br, or —I, or a salt or stereoisomer thereof;

iii) assaying an amount of binding between antibodies from the sample and the surface-bound moiety of the first substrate as compared to a reference, wherein the binding between the antibodies and the surface-bound moiety indicates the presence of antibodies in the sample that are reactive to the S-303-treated RBCs, wherein the reference is a second substrate that lacks the surface-bound moiety, and the second substrate is the same as the first substrate;

b) determining whether the amount of binding is higher than the reference; and

c) administering the red blood cell transfusion to the patient in accordance with the determination that the amount of binding is not higher than the reference.

2. The method of claim 1 , wherein R 21 is —C 1-8 alkyl-N(CH 2 CH 2 Cl) 2 .

3. The method of claim 1 , wherein the surface-bound moiety resulted from treating the first substrate with S-197:

4. The method of claim 1 , wherein the surface-bound moiety resulted from treating the first substrate with S-220:

5. The method of claim 1 , wherein the moiety bound to the surface of the first substrate is present at a loading level of at least about 10,000 moieties per red blood cell.

6. The method of claim 1 , wherein the moiety bound to the surface of the first substrate is present at a loading level of at least about 50 moieties/μm 2 .

7. The method of claim 1 , wherein the substrate comprises a polymeric particle, matrix, or portion of an assay plate.

8. The method of claim 1 , wherein the substrate comprises red blood cells.

9. The method of claim 1 , wherein step (ii) comprises contacting the sample and the substrate with anti-human globulin, and wherein step (iii) comprises assaying an amount of agglutination.

10. The method of claim 1 , wherein step (iii) further comprises assaying an amount of binding between antibodies from the sample and a third substrate having a reduced amount of the surface-bound moiety as compared with the first substrate.

Assignments (1)

Continuity (3)

References Cited (214)