FLUID CONNECTOR
Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.
1 - 276 . (canceled)
277 . A cartridge for use within a cell processing workcell, comprising:
a liquid transfer bus; and
a plurality of modules including an elutriation module and at least one module selected from the group consisting of a cell selection module, an electroporation module, and a bioreactor module,
wherein each module is fluidically coupled to the liquid transfer bus.
278 . The cartridge of claim 277 , wherein the cartridge comprises one or more sterile liquid transfer ports.
279 . The cartridge of claim 277 , wherein the at least one module of the plurality of modules of the cartridge comprises the bioreactor module.
280 . The cartridge of claim 277 , wherein the at least one module of the plurality of modules of the cartridge comprises the cell selection module.
281 . The cartridge of claim 280 , wherein the cell selection module is a magnetic-activated cell selection module.
282 . The cartridge of claim 281 , wherein the magnetic-activated cell selection module comprises a flow cell.
283 . The cartridge of claim 282 , wherein the flow cell is configured to interface with one or more magnet arrays of a corresponding instrument of the cell processing workcell to perform magnetic cell separation on cells within the flow cell.
284 . The cartridge of claim 282 , wherein the flow cell comprises a set of laminar fluid flow channels.
285 . The cartridge of claim 277 , wherein the at least one module of the plurality of modules of the cartridge comprises the electroporation module.
286 . The cartridge of claim 277 , further comprising a pump fluidically coupled to the liquid transfer bus.
287 . The cartridge of claim 286 , wherein a housing of the cartridge comprises an opening, and wherein at least a portion of the pump is arranged within the opening of the housing so as to be engageable to move fluid within the cartridge.
288 . The cartridge of claim 287 , wherein the portion of the pump arranged within the opening of the housing comprises tubing engageable to move the fluid within the cartridge.
289 . The cartridge of claim 277 , wherein the liquid transfer bus comprises a manifold, fluid conduits, and valves.
290 . The cartridge of claim 289 , wherein each of the valves is configured to receive an actuator of an instrument of the cell processing workcell, and wherein actuation of the actuator of the instrument causes the valve to move between a first position and a second position.
291 . The cartridge of claim 289 , wherein the manifold is disposed within an exterior wall of a housing of the cartridge.
292 . The cartridge of claim 277 , wherein the elutriation module is a counterflow centrifugal elutriation module comprising a rotor configured to separate cells from a fluid.
293 . The cartridge of claim 292 , wherein the counterflow centrifugal elutriation module further comprises one or more apertures configured to facilitate visualization of the rotor.
294 . The cartridge of claim 292 , wherein one or more portions of the rotor is optically transparent.
295 . The cartridge of claim 292 , wherein the rotor comprises an asymmetric shape.
296 . The cartridge of claim 292 , wherein the rotor is configured to separate the cells from the fluid based on one or more of cell cycle, cell size, cell type, and density.