PROCESS FOR PREPARING RASPBERRY KETONE
The present invention relates to a process for preparing natural raspberry ketone, comprising a step (a) of bioconversion of p-coumaric acid allowing the preparation of p-hydroxybenzaldehyde, a step (b) in which the p-hydroxybenzaldehyde obtained on conclusion of step (a) is condensed with acetone to allow the formation of p-hydroxybenzalacetone, a step (c) in which the p-hydroxybenzalacetone obtained on conclusion of step (b) is converted into raspberry ketone by bioconversion or biocatalysis.
1 . A process for preparing natural raspberry ketone, comprising:
a step (a) of bioconversion of p-coumaric acid allowing for preparation of p-hydroxybenzaldehyde,
a step (b) in which the p-hydroxybenzaldehyde obtained upon conclusion of step (a) is condensed with acetone to allow for formation of p-hydroxybenzalacetone,
a step (c) in which the p-hydroxybenzalacetone obtained upon conclusion of step (b) is converted into raspberry ketone by bioconversion or biocatalysis.
2 . The process according to claim 1 , in which step (a) and/or step (c) is a microbiological process.
3 . The process according to claim 1 , in which the bioconversion reaction is performed by fermentation in the presence of a microorganism.
4 . The process according to claim 1 , in which the condensation step (b) is performed in the presence of a biocatalyst.
5 . The process according to claim 1 , in which step (b) is performed in the presence of an amino acid.
6 . The process according to claim 1 , in which step (c) is performed in the presence of an ene-reductase or an enone-reductase.
7 . The process according to claim 1 , in which step (c) is performed in the presence of a cofactor.
8 . The process according to claim 1 , in which step (c) is performed in the presence of a yeast belonging to the group Saccharomyces or Candida.
9 . The process according to claim 1 , in which the p-hydroxybenzaldehyde obtained on conclusion of step (a) is used without further purification in step (b).
10 . The process according to claim 1 , in which the p-hydroxybenzalacetone obtained on conclusion of step (b) is used without further purification in step (c).
11 . The process according to claim 3 , wherein the microorganism comprises one or more microorganisms belonging to the order of Actinomycetales, the family Streptomycetacae, Pseudonocardiacae, or any combination thereof.
12 . The process according to claim 11 , wherein the microorganism comprises Streptomyces setonii, Amycolatopsis sp. Streptomyces psammoticus , or both.
13 . The process according to claim 11 , wherein the microorganism comprises a strain available under the number ATCC39116, DSMZ 9991, DSMZ 9992, CCTCC 2015329 or IMI 390106.
14 . The process according to claim 4 , wherein the biocatalyst is an albumin.
15 . The process according to claim 14 , wherein the albumin is bovine serum albumin.
16 . The process according to claim 8 , wherein the yeast comprises Saccharomyces cerevisiae, Candida lipolytica , or both.