Transgenic cory
The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
1 . A transgenic Cory whose genome comprises:
(a) a chromosomally integrated expression cassette encoding a red fluorescent protein, wherein the integrated expression cassette encoding the red fluorescent protein is designated as the “Red Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127094, wherein the transgenic Cory exhibits red fluorescence,
(b) a chromosomally integrated expression cassette encoding an orange fluorescent protein, wherein the integrated expression cassette encoding the orange fluorescent protein is designated as the “Orange Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127095, wherein the transgenic Cory exhibits orange fluorescence, or
(c) a chromosomally integrated expression cassette encoding a green fluorescent protein, wherein the integrated expression cassette encoding the green fluorescent protein is designated as the “Green Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127096, wherein the transgenic Cory exhibits green fluorescence.
2 . The transgenic Cory of claim 1 , further defined as a fertile, transgenic Cory.
3 . The transgenic Cory of claim 1 , wherein the transgenic Cory is homozygous for the integrated expression cassette.
4 . The transgenic Cory of claim 1 , wherein the transgenic Cory is heterozygous for the integrated expression cassette.
5 . A method of producing a transgenic Cory comprising:
(a) obtaining a transgenic Cory whose genome comprises:
(i) a chromosomally integrated expression cassette encoding a red fluorescent protein, wherein the integrated expression cassette encoding the red fluorescent protein is designated as the “Red Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number 127094, wherein the transgenic Cory exhibits red fluorescence,
(ii) a chromosomally integrated expression cassette encoding an orange fluorescent protein, wherein the integrated expression cassette encoding the orange fluorescent protein is designated as the “Orange Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127095, wherein the transgenic Cory exhibits orange fluorescence, or
(iii) a chromosomally integrated expression cassette encoding a green fluorescent protein, wherein the integrated expression cassette encoding the green fluorescent protein is designated as the “Green Cory 1 transformation event” comprised in the germ stem cells deposited as ATCC accession number PTA-127096, wherein the transgenic Cory exhibits green fluorescence, and
(b) breeding the obtained transgenic Cory from step (a) with a second Cory to provide:
(x) a transgenic Cory comprising the “Red Cory 1 transformation event” comprised in germ stem cell deposited as ATCC accession no. PTA-127094, wherein the transgenic Cory exhibits red fluorescence;
(y) a transgenic Cory comprising the “Orange Cory 1 transformation event” comprised in germ stem cell deposited as ATCC accession no. PTA-127095, wherein the transgenic Cory exhibits orange fluorescence; or
(z) a transgenic Cory comprising the “Green Cory 1 transformation event” comprised in germ stem cell deposited as ATCC accession no. PTA-127096,
wherein the transgenic Cory exhibits green fluorescence.
6 . The method of claim 5 , wherein the second Cory is a non-transgenic Cory.