EXTRACELLULAR VESICLE PREPARATIONS
Provided herein are solutions and dried forms of extracellular vesicles (EVs) that are useful as therapeutic agents, and therapeutic compositions thereof.
1 . A dried form comprising extracellular vesicles (EVs) from bacteria, wherein the dried form has a moisture content of below about 6%.
2 . The dried form of claim 1 , wherein the dried form has a moisture content of below about 5%.
3 . The dried form of claim 1 , wherein the dried form has a moisture content of below about 4%.
4 . The dried form of claim 1 , wherein the dried form has a moisture content of between about 1% to about 4%.
5 . The dried form of claim 1 , wherein the dried form has a moisture content of between about 2% to about 3%.
6 . The dried form of claim 1 , wherein the dried form comprises a powder.
7 . The dried form of claim 1 , wherein the dried form comprises a lyophilate.
8 . The dried form of claim 1 , wherein the dried form comprises EVs from a bacterial strain that is associated with mucus.
9 . The dried form of claim 1 , wherein the dried form comprises EVs from anaerobic bacteria.
10 . The dried form of claim 1 , wherein the anaerobic bacteria are obligate anaerobes.
11 . The dried form of claim 1 , wherein the anaerobic bacteria are facultative anaerobes.
12 . The dried form of claim 1 , wherein the anaerobic bacteria are aerotolerant anaerobes.
13 . The dried form of claim 1 , wherein the dried form comprises EVs from monoderm bacteria.
14 . The dried form of claim 1 , wherein the dried form comprises EVs from diderm bacteria.
15 . The dried form of claim 1 , wherein the dried form comprises EVs from Gram negative bacteria.
16 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the Prevotellaceae; Veillonellaceae; Tannerellaceae; Rikenellaceae; Selenomonadaceae; Sporomusaceae; Synergistaceae; Christensenellaceae; or Akkermaniaceae family.
17 . The dried form of claim 1 , wherein the dried form comprises EVs from Gram positive bacteria.
18 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the Oscillospiraceae; Clostridiaceae; or Lachnospiraceae family.
19 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the genus Prevotella.
20 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the genus Veillonella.
21 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the genus Parabacteroides.
22 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the Oscillospiraceae family.
23 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the Tannerellaceae family.
24 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the Prevotellaceae family.
25 . The dried form of claim 1 , wherein the dried form comprises EVs from bacteria of the Veillonellaceae family.
26 . A therapeutic composition comprising the dried form of any one of claims 1 to 25 .