BRASSICA NAPUS PLANTS COMPRISING AN IMPROVED FERTILITY RESTORER
The invention provides fertility restorer Brassica napus plants, plant material and seeds, characterized in that these products harbor a specific introgression fragment of the Ogura fertility restorer at the end of chromosome N10. Tools are also provided which allow detection of the fertility restorer.
1 . A Brassica napus plant comprising an Ogura restorer on chromosome N10.
2 . The Brassica plant of claim 1 , wherein the Ogura restorer is present at the end of chromosome N10.
3 . The Brassica plant according to claim 2 , wherein said Ogura restorer is present downstream of nucleotide 19,218,577 of chromosome N10.
4 . The Brassica plant according to claim 1 , wherein said Ogura restorer comprises markers M2, M3 and M5, and does not comprise markers M1 and M4.
5 . The Brassica plant according to claim 1 , wherein said Ogura restorer comprises a Raphanus chromosome fragment between position 8,330,119 and 10,655,049 of the Raphanus chromosome or a part thereof.
6 . The Brassica plant according to claim 1 , which is a Brassica napus WOSR plant or a Brassica napus SOSR plant.
7 . The Brassica plant according to claim 1 , wherein the Ogura restorer is obtainable from reference seeds deposited at NCIMB under accession number NCIMB 43628.
8 . The Brassica plant according to claim 1 , which restores the fertility of a CMS-Ogura Brassica napus plant.
9 . The Brassica plant according to claim 1 , wherein the Ogura restorer is present in homozygous form.
10 . The Brassica plant according to claim 9 , which is an inbred plant.
11 . The Brassica plant according to claim 1 , wherein the Ogura restorer is present in heterozygous form.
12 . The Brassica plant according to claim 11 , which is a hybrid plant, said hybrid plant optionally further containing CMS-Ogura.
13 . A part, seed or progeny of the Brassica plant according to claim 1 .
14 . Hybrid seed comprising the Ogura restorer as described in claim 1 .
15 . The Brassica plant, part seed or progeny thereof according to claim 1 , further comprising a technically induced mutant or a transgene.
16 . The Brassica plant according to claim 15 , wherein said technically induced mutant confers herbicide tolerance, or wherein said transgene is a gene conferring herbicide tolerance.
17 . A method for identifying a Brassica napus plant comprising the Ogura restorer according to claim 1 , said method comprising determining the presence of a Raphanus marker for Rfo-N10 in the genomic DNA of said plant.
18 . The method according to claim 17 , wherein said marker is a marker in the region comprising nucleotide 8,600,416 to 9,251,274 of Raphanus chromosome R09.
19 . The method according to claim 17 , wherein said marker is marker M2, M3 or M5.
20 . The method according to claim 17 , further comprising determining the absence of a Raphanus marker absent in Rfo-N10 in the genomic DNA of said plant.
21 . The method according to claim 18 , further comprising determining the absence of a Raphanus marker absent in Rfo-N10 in the genomic DNA of said plant, wherein said marker absent in Rfo-N10 is a marker in the region upstream of and including position 8,330,119 of Raphanus chromosome R09, or is a marker in the region downstream of and including position 10,655,049 excluding position 15,447,221-15,450,692.
22 . The method according to claim 19 , further comprising determining the absence of a Raphanus marker absent in Rfo-N10 in the genomic DNA of said plant, wherein said marker absent in Rfo-N10 is marker M1 or M4.
23 . A method for selecting a Brassica napus plant comprising an Ogura restorer, said method comprising identifying the presence of a Raphanus marker for Rfo-N10 as described in claim 17 , and selecting a Brassica napus plant comprising said Raphanus marker for Rfo-N10.
24 . A method for producing a Brassica napus plant comprising an Ogura restorer, said method comprising:
a. crossing a first Brassica plant according to claim 1 with a second Brassica napus plant; and
b. identifying, and optionally selecting, a progeny plant comprising Rfo-N10 as described in claim 17 .
25 . A method for producing hybrid Brassica napus seed, said method comprising:
a. providing a male Brassica napus plant comprising the Ogura restorer according to claim 1 , wherein said Ogura restorer is present in homozygous form;
b. providing a female Brassica napus plant comprising CMS-Ogura;
c. crossing said female Brassica napus plant with said male Brassica napus plant; and optionally
d. harvesting seeds.
26 . Hybrid Brassica napus seed produced with the method according to claim 25 .
27 . A hybrid Brassica napus plant produced from the seed according to claim 26 .
28 . (canceled)
29 . (canceled)
30 . A method for the protection of a group of cultivated plants according to claim 16 in a field, said method comprising controlling weeds by applying a composition comprising one or more herbicidal active ingredients.
31 . The method according to claim 30 , wherein the plants comprise a technically induced mutant which confers tolerance to imidazolinone and wherein the herbicide is an imidazolinone; or wherein the plants comprise a gene which confers resistance to glufosinate or to glufosinate ammonium and where the herbicide is glufosinate or glufosinate ammonium, or wherein the plants comprise a gene conferring resistance to glyphosate, and the herbicide is glyphosate.