Oil-based adjuvants
The instant invention provides various formulations comprising combinations of immunostimulating oligonucleotides, polycationic carriers, sterols, saponins, quaternary amines, TLR-3 agonists, glycolipids, and MPL-A or analogs thereof in oil emulsions, use thereof in preparations of immunogenic compositions and vaccines, and use thereof in the treatment of animals.
1. A vaccine composition comprising a soluble G protein of Hendra virus and an oil-based adjuvant, comprising an oily phase present in the amount of at least 45% v/v of the vaccine composition and comprising an immunostimulatory oligonucleotide and a polycationic carrier, wherein said vaccine composition is a water-in-oil emulsion.
2. The vaccine composition of claim 1 , wherein the polycationic carrier is dextran DEAE and the immunostimulatory oligonucleotide is a CpG.
3. The vaccine composition of claim 1 wherein the oily phase comprises at least 48% v/v of said vaccine composition.
4. The vaccine composition of claim 1 wherein the oily phase comprises at least 50% v/v of said vaccine composition.
5. The vaccine composition of claim 2 wherein the oily phase comprises 50-52% of said vaccine composition.
6. A method for prevention of an infection caused by Nipah virus or Hendra virus, said method comprising administering to a subject in need thereof a vaccine composition according to claim 1 .
7. The method of claim 6 for prevention of the infection caused by Nipah virus, wherein the subject is a pig.