Automated calibration of laser system and tomography system with fluorescent imaging of scan pattern
A laser system calibration method and system are provided. In some methods, a calibration plate may be used to calibrate a video camera of the laser system. The video camera pixel locations may be mapped to the physical space. A xy-scan device of the laser system may be calibrated by defining control parameters for actuating components of the xy-scan device to scan a beam to a series of locations. Optionally, the beam may be scanned to a series of locations on a fluorescent plate. The video camera may be used to capture reflected light from the fluorescent plate. The xy-scan device may then be calibrated by mapping the xy-scan device control parameters to physical locations. A desired z-depth focus may be determined by defining control parameters for focusing a beam to different depths. The video camera or a confocal detector may be used to detect the scanned depths.
1. A method of calibrating a laser system with a Z-depth, comprising:
placing a laser system contact at a predetermined distance from a plate;
placing a fluid on the plate between the plate and the laser system contact;
incrementally adding the fluid on the plate to increase a fluid level by incremental amounts to a plurality of known fluid levels;
at each of the plurality of known fluid levels, detecting the fluid level using an imaging system of the laser system; and
correlating the fluid levels detected by the imaging system with the known fluid levels.
2. The method of claim 1 , wherein the step of placing the laser system contact at the predetermined distance from the plate includes:
placing a disk with a predetermined depth between the laser system contact and the plate, the disk contacting the plate;
positioning the laser system contact against the disk; and
removing the disk.
3. The method of claim 2 , wherein the disk has a depth of 4-15 mm.
4. The method of claim 2 , wherein the disk has a depth of 6-10 mm.
5. The method of claim 1 , wherein the fluid level is increased by one mm increments.
6. The method of claim 5 , wherein the fluid level is increased in three increments of one mm.
7. The method of claim 1 , wherein the fluid is water.
8. The method of claim 1 , wherein the imaging system of the laser system is an OCT (optical coherence tomography) imaging system.