Integrated system for processing microfluidic samples, and method of using same
This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
1. An apparatus for processing and amplifying a plurality of polynucleotide-containing samples, the apparatus comprising:
a multi-lane microfluidic cartridge configured to process and amplify the plurality of polynucleotide-containing samples, wherein the multi-lane microfluidic cartridge comprises multiple layers;
a reagent reservoir;
a bay configured to receive the multi-lane microfluidic cartridge, the multi-lane microfluidic cartridge comprising a plurality of sample lanes, each sample lane of the plurality of sample lanes comprising a dedicated pipette inlet and a region where amplification occurs, the multi-lane microfluidic cartridge further configured to permit the plurality of polynucleotide-containing samples to be loaded into the multi-lane microfluidic cartridge at different times, wherein a first sample of the plurality of polynucleotide-containing samples is passed within the multi-lane microfluidic cartridge at a different time than a second sample of the plurality of polynucleotide containing samples;
a first heat source separate from the multi-lane microfluidic cartridge and configured to apply heat to the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is received in the bay, the first heat source configured to apply the heat to one or more selected regions of the multi-lane microfluidic cartridge at one or more selected times, in order to prepare one or more polynucleotides from the plurality of polynucleotide-containing samples for amplification;
a plurality of second heat sources separate from the multi-lane microfluidic cartridge and configured to apply heat to one or more selected regions of the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is received in the bay, the plurality of second heat sources configured to apply the heat to the one or more selected regions of the multi-lane microfluidic cartridge at one or more selected times, in order to amplify the prepared one or more polynucleotides;
wherein a second heat source of the plurality of second heat sources is configured to cyclically heat in a series of heating phases after the multi-lane microfluidic cartridge is received in the bay, wherein each heating phase comprises the second heat source being cycled between at least two temperatures;
wherein the second heat source of the plurality of second heat sources is configured to maintain a substantially uniform temperature within a region where amplification occurs at each temperature of the at least two temperatures when the multi-lane microfluidic cartridge is received in the bay;
wherein the plurality of second heat sources are configured to perform amplification reactions in the plurality of sample lanes when the multi-lane microfluidic cartridge is received in the bay;
wherein a sample lane of the multi-lane microfluidic cartridge comprises a processing chamber, and wherein the first heat source is configured to apply heat to the processing chamber when the multi-lane microfluidic cartridge is received in the bay;
a magnet configured to move into and out of place to apply a magnetic field to a polynucleotide-loaded retention member in the processing chamber when the multi-lane microfluidic cartridge is received in the bay and the polynucleotide-loaded retention member is received in the multi-lane microfluidic cartridge; and
an optical detector configured to detect the presence of the one or more amplified polynucleotides within the region where amplification occurs.
2. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge comprises a registration member that fits into a complementary feature of the bay.
3. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge comprises a barcode label.
4. The apparatus of claim 1 , further comprising a sample container.
5. The apparatus of claim 4 , wherein the sample container comprises a barcode label.
6. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge is packaged in a sealed pouch.
7. The apparatus of claim 1 , further comprising a bar code reader.
8. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge is configured for sample in, result out style operations.
9. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge is configured for bulk lysis.
10. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge is configured for DNA capture, wash, and release.
11. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge is configured for PCR preparation and execution.
12. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge is configured for use without manual interaction except during the act of injecting a sample into the device.
13. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge comprises hydrophobic vents.
14. The apparatus of claim 13 , wherein the hydrophobic vents are configured to remove trapped air formed during processing.
15. The apparatus of claim 1 , wherein the reagent reservoir comprises a wash buffer reservoir, and wherein the plurality of sample lanes are configured to share a wash buffer received in the multi-lane microfluidic from the wash buffer reservoir.
16. The apparatus of claim 1 , wherein the reagent reservoir comprises a release reagent reservoir, and wherein the plurality of sample lanes are configured to share a release reagent received in the multi-lane microfluidic cartridge from the release reagent reservoir.
17. The apparatus of claim 1 , wherein the reagent reservoir comprises a hydroxide solution reservoir, and wherein the plurality of sample lanes are configured to share a hydroxide solution received in the multi-lane microfluidic from the reagent reservoir.
18. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge is disposable.
19. The apparatus of claim 1 , wherein the multi-lane microfluidic cartridge comprises a waste reservoir and a channel extending between the processing chamber and the waste reservoir.
20. The apparatus of claim 19 , further comprising a valve configured to inhibit motion of a material in the channel when the multi-lane microfluidic cartridge is received in the bay.