Granted Patent B2

US 12,577,608 · App. 18/328,489 · Granted Mar 17, 2026

Inventors: Tural Aksel (Redwood City, CA); Torri Rinker (San Francisco, CA); Markus Burns (Palo Alto, CA); Michael Dorwart (San Jose, CA); Rachel Galimidi (Belmont, CA); Dmitriy Gremyachinskiy (San Diego, CA); Stephen Hendricks (Los Gatos, CA); Elvis Ikwa (San Leandro, CA); Gregory Kapp (San Carlos, CA); Joshua Simon Klein (Pacifica, CA); Julia Robinson (East Palo Alto, CA); Cassandra Stawicki (Foster City, CA); Sonal Tonapi (Santa Clara, CA); Parag Mallick (San Mateo, CA)

Assignee: Nautilus Subsidiary, Inc.

C12Q1/6832C12Q1/6834C12Q1/6876

Abstract

Claims (34)

1 . A method for detecting analytes comprising:

providing an array of different analytes attached to a solid support, wherein the different analytes are attached to the solid support by structured nucleic acid particles,

delivering to the array of different analytes a fluidic medium comprising a plurality of detectable probes, each of the detectable probes comprising a retaining component attached to a binding component and a plurality of label components, each binding component of the plurality of detectable probes is configured to bind to two or more different analytes of the array of different analytes, and

after delivering the fluidic medium to the array of different analytes, acquiring signals from label components of the detectable probes bound to analytes of the array of different analytes, thereby detecting at least two different analytes of the array of different analytes,

wherein the retaining component comprises a first face and a second face, wherein the binding component is attached to the first face of the retaining component, and wherein the plurality of label components is attached to the second face of the retaining component.

2 . The method of claim 1 , wherein an analyte of the different analytes comprises a polypeptide, a protein, a peptide, an oligopeptide, a nucleic acid, or an oligonucleotide.

3 . The method of claim 2 , wherein the analyte comprises a polypeptide.

4 . The method of claim 1 , further comprising quantifying the different analytes.

5 . The method of claim 1 , further comprising determining the location of the different analytes on the solid support.

6 . The method of claim 1 , wherein the array of different analytes comprises at least 10 6 analytes.

7 . The method of claim 1 , wherein an analyte of the different analytes comprises a single molecule.

8 . The method of claim 1 , wherein a label component of the plurality of label components comprises a fluorophore, luminophore, chromophore, nanoparticle, heavy atom, radioactive isotope, mass label, charge label, spin label, receptor, ligand, nucleic acid barcode, polypeptide barcode, or polysaccharide barcode.

9 . The method of claim 8 , wherein the label component comprises a fluorophore.

10 . The method of claim 8 , wherein the label component comprises a nucleic acid barcode.

11 . The method of claim 10 , wherein the signal from the nucleic acid barcode is amplified.

12 . The method of claim 1 , wherein the structured nucleic acid particles each comprise nucleic acid origami or a nucleic acid nanoball.

13 . The method of claim 1 , wherein the retaining component of each of the detectable probes comprises nucleic acid origami or a nucleic acid nanoball.

14 . The method of claim 1 , wherein each of the detectable probes comprise two or more binding components.

15 . The method of claim 1 , wherein the binding component comprises an antibody, antibody fragment, lectin, functional lectin fragment, avidin, streptavidin, or aptamer.

16 . The method of claim 1 , wherein the solid support is non-porous.

17 . The method of claim 1 , wherein the solid support comprises glass, plastic, nylon, ceramic, silica, silicon, carbon, metal, or a polymer.

18 . The method of claim 1 , wherein the array of different analytes comprises sites that are separated by less than 100 microns, 50 microns, 10 microns, 5 microns, 1 micron, or 0.5 micron.

19 . A composition comprising:

an array of different analytes attached to a solid support, wherein the different analytes are attached to the solid support by structured nucleic acid particles, and

a fluidic medium comprising a plurality of detectable probes contacted to the array of different analytes, detectable probes of the plurality of detectable probes being unattached to the solid support, each of the detectable probes comprising a retaining component attached to one or more binding components and a plurality of label components, each of the one or more binding components is configured to bind to two or more different analytes of the array of different analytes,

wherein the retaining component comprises a first face and a second face, wherein the one or more binding component is attached to the first face of the retaining component, and wherein the plurality of label components is attached to the second face of the retaining component.

20 . The method of claim 19 , wherein each binding component of the plurality of detectable probes recognizes an epitope common to the two or more different analytes of the array of different analytes.

21 . The method of claim 20 , wherein the epitope contains a contiguous 3, 4, or 5 amino acid sequence.

22 . The method of claim 20 , wherein the epitope contains a post-translational modification.

23 . A system, comprising:

an array of structured nucleic acid particles attached to a solid support, wherein structured nucleic acid particles of the array of structured nucleic acid particles are attached to a plurality of analytes, wherein each of the structured nucleic acid particles is detectable at single-analyte resolution;

a plurality of detectable probes, each of the detectable probes comprising a retaining component attached to one or more binding components and a plurality of label components, each of the one or more binding components is configured to bind to two or more different analytes of the plurality of analytes, wherein the retaining component comprises a first face and a second face, wherein the one or more binding components are attached to the first face of the retaining component, and wherein the plurality of label components is attached to the second face of the retaining component;

a fluidic device that is configured to deliver the plurality of detectable probes to the array of structured nucleic acid particles, and

a detection system that is configured to detect signals from detectable probes bound to analytes on the array of structured nucleic acid particles at single-analyte resolution.

Assignments (2)

Continuity (5)

References Cited (179)