MICROBIAL CELLS, METHODS OF PRODUCING THE SAME, AND USES THEREOF
The present invention relates to microbial cells, including but not limited to aerobic bacteria cells and anaerobic bacteria cells, as well as yeast cells, and methods for producing the cells, feed additives and compositions comprising the cells, and uses involving administration of the cells to animals.
1 - 119 . (canceled)
120 . A feed additive comprising viable freeze-dried Megasphaera elsdenii cells, wherein the freeze-dried Megasphaera elsdenii cells remain viable after storage at a temperature of about 25° C. for at least 2 weeks, and wherein the feed additive comprises about 1×10 7 to about 1×10 12 CFU/g of viable cells.
121 . The feed additive of claim 120 , wherein the freeze-dried Megasphaera elsdenii cells remain viable after storage at about 4° C. for at least 1 month.
122 . The feed additive of claim 120 , wherein the feed additive further comprises one or more of another microorganism, growth substrates, enzymes, sugars, carbohydrates, extracts, and growth promoting micro-ingredients, or combinations thereof.
123 . The feed additive of claim 120 , wherein the feed additive is selected from the group consisting of: a powder, a granulate, a particulate, a pellet, a cake, a water soluble concentrate, a paste, a bolus, a tablet, a dust, a liquid, a component thereof, or combinations thereof.
124 . The feed additive of claim 120 , wherein the feed additive is a probiotic.
125 . A feed additive comprising viable encapsulated freeze-dried Megasphaera elsdenii cells, wherein the encapsulated freeze-dried Megasphaera elsdenii cells remain viable after storage at a temperature of about 25° C. for at least 2 weeks, and wherein the feed additive comprises about 1×10 7 to about 1×10 12 CFU/g of viable cells.
126 . The feed additive of claim 125 , wherein the encapsulated freeze-dried Megasphaera elsdenii cells remain viable after storage at about 4° C. for at least 1 month.
127 . The feed additive of claim 125 , wherein the feed additive further comprises one or more of another microorganism, growth substrates, enzymes, sugars, carbohydrates, extracts, and growth promoting micro-ingredients, or combinations thereof.
128 . The feed additive of claim 125 , wherein the feed additive is selected from the group consisting of: a powder, a granulate, a particulate, a pellet, a cake, a water soluble concentrate, a paste, a bolus, a tablet, a dust, a liquid, a component thereof, or combinations thereof.
129 . The feed additive of claim 125 , wherein the feed additive is a probiotic.
130 . A feed additive comprising viable freeze-dried anaerobic bacterial cells, wherein the freeze-dried anaerobic bacterial cells remain viable after storage at a temperature of about 25° C. for at least 2 weeks, and wherein the feed additive comprises about 1×10 7 to about 1×10 12 CFU/g of viable cells.
131 . The feed additive of claim 130 , wherein the freeze-dried anaerobic bacterial cells remain viable after storage at about 4° C. for at least 1 month.
132 . The feed additive of claim 130 , wherein the anaerobic bacterial cells are selected from the group consisting of: Megasphaera elsdenii, Bifidobacterium breve, Lactobacillus plantarum, Bifidobacterium animalis subsp. lactis, Pediococcus acidilactici , and Lactobacillus casei.
133 . The feed additive of claim 130 , wherein the feed additive further comprises one of more of another microorganism, growth substrates, enzymes, sugars, carbohydrates, extracts, and growth promoting micro-ingredients, or combinations thereof.
134 . The feed additive of claim 130 , wherein the feed additive is selected from the group consisting of: a powder, a granulate, a particulate, a pellet, a cake, a water soluble concentrate, a paste, a bolus, a tablet, a dust, a liquid, a component thereof, or combinations thereof.
135 . The feed additive of claim 130 , wherein the feed additive is a probiotic.
136 . A feed additive comprising viable encapsulated freeze-dried anaerobic bacterial cells, wherein the encapsulated freeze-dried anaerobic bacterial cells remain viable after storage at a temperature of about 25° C. for at least 2 weeks, and wherein the feed additive comprises about 1×10 7 to about 1×10 12 CFU/g of viable cells.
137 . The feed additive of claim 136 , wherein the encapsulated freeze-dried anaerobic bacterial cells remain viable after storage at about 4° C. for at least 1 month.
138 . The feed additive of claim 136 , wherein the anaerobic bacterial cells are selected from the group consisting of: Megasphaera elsdenii, Bifidobacterium breve, Lactobacillus plantarum, Bifidobacterium animalis subsp. lactis, Pediococcus acidilactici , and Lactobacillus casei.
139 . The feed additive of claim 136 , wherein the feed additive further comprises one or more of another microorganism, growth substrates, enzymes, sugars, carbohydrates, extracts, and growth promoting micro-ingredients, or combinations thereof.
140 . The feed additive of claim 136 , wherein the feed additive is selected from the group consisting of: a powder, a granulate, a particulate, a pellet, a cake, a water soluble concentrate, a paste, a bolus, a tablet, a dust, a liquid, a component thereof, or combinations thereof.
141 . The feed additive of claim 136 , wherein the feed additive is a probiotic.