COMPETITIVE METHODS AND COMPOSITIONS FOR AMPLIFYING POLYNUCLEOTIDES
Disclosed herein, inter alia, are methods for increasing monoclonal nucleic acid amplification products on a solid support.
1 . A method of differentially amplifying a first plurality of polynucleotides relative to a second plurality of polynucleotides on a solid support, wherein said solid support comprises
a first plurality of polynucleotides, wherein each polynucleotide in said first plurality of polynucleotides comprise a first platform primer sequence immobilized to a solid support, a first template sequence, and a second platform primer binding sequence;
a second plurality of polynucleotides, wherein each polynucleotide in said second plurality of polynucleotides comprise the first platform primer sequence, a second template sequence, and a third platform primer binding sequence;
a population of first platform primers, a population of second platform primers, and a population of third platform primers, wherein each of said first platform primers, said second platform primers, and said third platform primers are immobilized to said solid support; said method comprising:
(i) contacting said solid support with a plurality of blocking elements thereby forming a plurality of blocked polynucleotide complexes, wherein each of said blocked polynucleotide complexes comprise a blocking element bound to one of said third platform primer binding sequences of said second polynucleotide; or
(ii) contacting said solid support with a plurality of blocking elements thereby forming a plurality of blocked third platform primer complexes, wherein each of said blocked third platform primer complexes comprises a blocking element bound to one of said third platform primers; and
amplifying said first plurality of polynucleotides;
thereby differentially amplifying the first plurality of polynucleotides relative to a second plurality of polynucleotides on a solid support.
2 . The method of claim 1 , wherein the amplifying comprises a plurality of amplification cycles, each cycle comprising hybridizing the first platform primer binding sequence of the first polynucleotide to a first platform primer, extending the first platform primer with a polymerase to form an amplification product hybridized to the first polynucleotide; and denaturing the amplification product from the first polynucleotide.
3 . The method of claim 2 , further comprising contacting said solid support with said plurality of blocking elements within each amplification cycle.
4 . The method of claim 1 , further comprising removing the blocking elements and amplifying the second plurality of immobilized polynucleotides.
5 . The method of claim 1 , further comprising
(iii) contacting said solid support with a second plurality of blocking elements thereby forming a plurality of second blocked polynucleotide complexes, wherein each of said second blocked polynucleotide complexes comprise a second blocking element bound to one of said second platform primer binding sequences of said first polynucleotide; or
(iv) contacting said solid support with a second plurality of blocking elements thereby forming a plurality of second blocked second platform primer complexes, wherein each of said blocked second platform primer complexes comprises a second blocking element bound to one of said population of second platform primers; and
amplifying said second plurality of polynucleotides.
6 . The method of claim 1 , wherein the blocking element and the second blocking elements are a protein or an oligonucleotide.
7 . The method of claim 1 , wherein the blocking element and the second blocking elements are an oligonucleotide comprising a complementary sequence to the third platform primer, or a complement thereof.
8 . The method of claim 1 , wherein the blocking element and the second blocking elements independently comprise locked nucleic acids (LNAs), Bis-locked nucleic acids (bisLNAs), twisted intercalating nucleic acids (TINAs), bridged nucleic acids (BNAs), 2′-O-methyl RNA:DNA chimeric nucleic acids, minor groove binder (MGB) nucleic acids, morpholino nucleic acids, C5-modified pyrimidine nucleic acids, peptide nucleic acids (PNAs), phosphorothioate nucleic acids, or combinations thereof.
9 . The method of claim 1 , wherein the blocking element and the second blocking element independently comprise one or more locked nucleic acids (LNAs), 2-amino-deoxyadenosine (2-amino-dA), trimethoxystilbene-functionalized oligonucleotides (TFOs), Pyrene-functionalized oligonucleotides (PFOs), peptide nucleic acids (PNAs), or aminoethyl-phenoxazine-dC (AP-dC) nucleic acids.
10 . The method of claim 1 , wherein the blocking element and the second blocking element are independently an oligonucleotide comprising about 5 to about 35 nucleotides.
11 . The method of claim 10 , wherein the blocking element and the second blocking element are independently a non-extendable oligonucleotide.
12 . The method of claim 1 , wherein the amplifying comprises 1 to 100 bridge-PCR amplification cycles.
13 . The method of claim 1 , wherein the amplifying comprises about 5 minutes to about 4 hours of solid-phase rolling circle amplification (RCA), solid-phase exponential rolling circle amplification (eRCA), solid-phase recombinase polymerase amplification (RPA), solid-phase helicase dependent amplification (HDA), or template walking amplification.
14 . A method of amplifying a polynucleotide on a solid support comprising a plurality of immobilized platform primers, said method comprising:
a) hybridizing a second platform primer binding sequence of a first immobilized polynucleotide to a second platform primer; wherein the first immobilized polynucleotide comprises a first platform primer sequence immobilized to a solid support, a first template sequence, and said second platform primer binding sequence;
b) binding a blocking element to a third platform primer binding sequence of a second immobilized polynucleotide or binding a blocking element to a third platform primer;
wherein the second immobilized polynucleotide comprises the first platform primer sequence, a second template sequence, and said third platform primer binding sequence;
c) extending the second platform primer with a polymerase to form a first amplification product; and
d) repeating steps a), b), and c), thereby amplifying the first amplification product on the solid support.
15 . The method of claim 14 , further comprising removing the blocking element and hybridizing a third platform primer binding sequence of the second immobilized polynucleotide to the third platform primer and extending the second immobilized primer with a polymerase to form a second amplification product.
16 . The method of claim 14 , wherein the solid support comprises a population of first platform primers, a population of second platform primers, and a population of third platform primers, wherein each of said first platform primers, said second platform primers, and said third platform primers are immobilized to said solid support.
17 . The method of claim 1 , further comprising binding a blocking element to a second platform primer binding sequence of the first immobilized polynucleotide or binding a blocking element to the second platform primer and amplifying the second immobilized polynucleotide.
18 . A method of forming a first immobilized polynucleotide and a second immobilized polynucleotide on a solid support, said method comprising:
contacting a solid support with a first polynucleotide and a second polynucleotide, wherein the solid support comprises a population of first platform primers, a population of second platform primers, and a population of third platform primers, wherein each of said first platform primers, said second platform primers and said third platform primers are immobilized to said solid support;
hybridizing a first platform primer binding sequence of the first polynucleotide to one of said first platform primers, wherein the first polynucleotide comprises said first platform primer binding sequence, a first template sequence, and a second platform primer sequence;
hybridizing a first platform primer binding sequence of the second polynucleotide to one of said second platform primers, wherein the second polynucleotide comprises said first platform primer binding sequence, a second template sequence, and a third platform primer sequence;
extending the first platform primer with a polymerase to form the first immobilized polynucleotide comprising the first platform primer sequence, a complement of the first template sequence, and a second platform primer binding sequence;
extending the second platform primer with a polymerase to form the second immobilized polynucleotide comprising the first platform primer sequence, a complement of second the template sequence, and a third platform primer binding sequence;
(i) binding a blocking element to a third platform primer binding sequence of said second immobilized polynucleotide or
(ii) binding a blocking element to a third platform primer; and
amplifying the first immobilized polynucleotide.
19 . A solid support comprising a plurality of amplification sites, wherein each amplification site comprises a population of first platform primers, a population of second platform primers, and a population of third platform primers, wherein each of the third platform primers comprise blocking element bound thereto.
20 . The solid support of claim 19 , wherein the solid support is a flow cell, particle, chip, slide, multi-well container, or unpatterned solid support.