CARTRIDGE FOR TISSUE TREATMENT
One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with a raised processing opening, a cup that can be received within the raised processing opening and has a screen, and a well plate that is positioned under the cover, has a well, and rotates relative to the cover such that the well aligns with the raised processing opening. One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with an opening that can receive a tissue sample.
1 . A cartridge for preparation of a regenerative epidermal suspension, comprising:
a cover comprising a raised processing opening;
a cup configured to be received within the raised processing opening, wherein the cup comprises a screen; and
a well plate positioned under the cover and comprising a well, the well plate configured to rotate relative to the cover such that the well aligns with the raised processing opening.
2 . The cartridge of claim 1 , wherein the screen is positioned transversely within the cup.
3 . The cartridge of claim 1 , wherein the screen is configured to separate out particulates above 100 microns in size.
4 . The cartridge of claim 1 , wherein the screen is configured to separate out particulates above 50 microns in size.
5 . The cartridge of claim 1 , wherein the screen is configured to separate out particulates above 10 microns in size.
6 . The cartridge of claim 1 , further comprising a docking spindle configured to connect to a base unit.
7 . The cartridge of claim 6 , wherein the docking spindle is configured to rotate the well plate.
8 . The cartridge of claim 1 , wherein the well comprises raised processing elements configured to enhance disaggregation of a tissue sample.
9 . The cartridge of claim 8 , wherein the raised processing elements are configured to enhance disaggregation of a tissue sample subjected to vertical, rotational, or grinding forces.
10 . The cartridge of any one of claim 1 wherein the cup is configured to be raised or lowered within the raised processing opening to position the cup in the well.
11 . The cartridge of claim 1 , wherein the cover comprises a plurality of openings.
12 . The cartridge of claim 11 , wherein the cover comprises a buffer opening.
13 . The cartridge of claim 1 , wherein the well plate comprises a plurality of openings.
14 . The cartridge of claim 13 , wherein the well plate comprises a buffer well and an enzyme well.
15 . The cartridge of claim 14 , wherein the well plate is configured to rotate such that the buffer well is positioned beneath the buffer opening.
16 . The cartridge of claim 14 , wherein the well plate is configured to rotate such that the enzyme well is positioned beneath the raised processing opening.
17 . The cartridge of claim 1 , wherein the cup is configured to receive a tissue disaggregator.
18 . A cartridge for preparation of a regenerative epidermal suspension, comprising:
a cover comprising an opening configured to receive a tissue sample; and
a well plate positioned under the cover and comprising a well, the well plate configured to be rotated relative to the cover such that the well aligns with the opening.
19 . The cartridge of claim 18 , wherein the cartridge is configured to lower the tissue sample into the well aligned with the opening.
20 . The cartridge of claim 18 , wherein the well plate comprises a buffer well and an enzyme well, and the well plate is configured to rotate to align either the buffer well or the enzyme well with the opening.
21 . The cartridge of claim 18 , further comprising a cup receivable within the opening of the cover, the cup being moveable between a raised position above the well to a lowered position within the well.