Recombinant algae having high lipid productivity
The invention involves the provision of recombinant algal mutants that have a genetic modification to a nucleic acid sequence encoding a trehalose biosynthetic enzyme, and/or a genetic modification to a nucleic acid encoding an RNA binding domain And in some embodiments either of these algal mutants can further have a genetic mutation to a nucleic acid sequence encoding an SGI1 polypeptide. Attenuation of one, two, or all three of these genes results in a mutant organism with increased lipid productivity. It was also discovered that one, two, three, or more genetic mutations can be accumulated or “stacked” in a particular mutant cell or organism to result in further increases in the production of lipid products. The lipid products of these mutants are useful as biofuels or for other specialty chemical products.
1. A method of producing a composition containing lipids comprising:
cultivating a recombinant Chlorophyte algal organism comprising a disruption in a nucleic acid sequence encoding an RNA binding domain having at least 90% sequence identity to SEQ ID NO: 1, wherein the recombinant Chlorophyte alga exhibits increased lipid productivity versus a corresponding control algal cell not having the disruption; and
thereby producing a composition containing lipids.
2. The method of claim 1 wherein the recombinant Chlorophyte algal organism further comprises a disruption in a nucleic acid sequence encoding an Significant Growth Improvement 1 (SGI1) polypeptide having at least 90% sequence identity to SEQ ID NO: 8.
3. The method of claim 2 wherein the recombinant Chlorophyte alga has at least 5 grams per square meter per day of lipid production.
4. The method of claim 2 , wherein the recombinant Chlorophyte alga has higher biomass productivity per unit time versus a corresponding control algal cell not having the disruption.
5. The method of claim 2 wherein the recombinant alga has higher total organic carbon production when cultivated under nitrogen deplete conditions.
6. The method of claim 2 wherein the recombinant alga is a Chlorophyte alga of a genus selected from the group consisting of: Chorella, Parachlorella, Picochlorum, Tetraselmis , and Oocystis.
7. The method of claim 1 further comprising a step of treating the recombinant Chlorophyte algal organism with ultraviolet radiation prior to the step of cultivating.
8. The method of claim 1 further comprising harvesting a lipidic composition from the recombinant Chlorophyte algal organism.
9. The method of claim 1 wherein the recombinant Chlorophyte algal cell is an alga of the genus Parachlorella.
10. The method of claim 9 , wherein the recombinant Chlorophyte alga has higher biomass productivity when cultivated under nitrogen deplete conditions.
11. The method of claim 1 wherein the recombinant Chlorophyte alga has at least 50% greater lipid productivity versus the control alga.
12. The method of claim 1 , wherein the recombinant Chlorophyte alga is of the Class Trebouxiophyceae.