AROMATIC-CATIONIC PEPTIDES AND USES OF SAME
The disclosure provides compositions and methods relating to aromatic-cationic peptides. The methods comprise administering to the subject an effective amount of an aromatic-cationic peptide to subjects in need thereof. For example, the peptides may be administered to subjects in need of a mitochondrial-targeted antioxidant.
1 . An aromatic-cationic peptide of the sequence
D-Arg-Tyr-Lys-Phe-NH 2 ,
or a pharmaceutically acceptable salt thereof.
2 . A pharmaceutical composition comprising the aromatic-cationic peptide of claim 1 and pharmaceutically acceptable salts thereof.
3 . The pharmaceutical composition of claim 2 further comprising a pharmaceutically acceptable carrier.
4 . A method of reducing the number of mitochondria undergoing mitochondrial permeability transition (MPT), or preventing mitochondrial permeability transitioning in a mammal in need thereof, the method comprising administering to the mammal an effective amount of the aromatic-cationic peptide of claim 1 .
5 . A method for reducing oxidative damage in a mammal in need thereof, the method comprising administering to the mammal an effective amount of the aromatic-cationic peptide of claim 1 .
6 . A method for increasing the ATP synthesis rate in a mammal in need thereof, the method comprising administering to the mammal an effective amount of the aromatic-cationic peptide of claim 1 .
7 . A method for determining the presence or amount of an administered aromatic-cationic peptide in a subject, the method comprising: detecting the administered aromatic-cationic peptide in a biological sample from the subject, wherein the aromatic-cationic peptide is
D-Arg-Tyr-Lys-Phe-NH 2
8 . The method of claim 7 , wherein detecting is performed during administration of the peptide.
9 . The method of claim 7 , wherein detecting is performed after administration of the peptide.
10 . The method of any one of claim 7 , wherein detecting comprises HPLC.
11 . The method of claim 10 , wherein the HPLC comprises reverse phase HPLC.
12 . The method of claim 10 , wherein the HPLC comprises ion exchange HPLC.
13 . The method of claim 7 , wherein detecting comprises mass spectrometry.
14 . The method of claim 7 , wherein the biological sample comprises a fluid.
15 . The method of claim 7 , wherein the biological sample comprises a cell.
16 . The method of claim 7 , wherein the biological sample comprises a tissue.
17 . The method of any one of claims 7 , wherein the biological sample comprises a biopsy.