PH-BASED STRUCTURAL CHANGE OF WATER SOLUBLE CARBON NANOPARTICLES FOR ENCAPSULATION AND RELEASE OF THERAPEUTIC AGENTS IN BIOLOGICAL SYSTEMS
Water soluble graphene oxide nanoparticles (GO) are provided having graphene sheets containing carboxylic and hydroxyl groups. The diameter of the GO when present in a closed form range from about 40 nm to 120 nm. Eco-friendly methods are provided for producing the GO. Methods for reversible encapsulation of a molecule within water soluble carbon nanoparticles (wsCNP) including the GO nanoparticles are provided that allow for delivery of the wsCNP loaded with therapeutic and/or imaging agents to a subject in need by releasing the therapeutic/imaging agent upon an increase in pH above about 7.2. The wsCNP have been shown to cross the blood brain barrier in a mouse model of vascular dementia, and methods are provided for delivering the wsCNP loaded with a therapeutic and/or imaging molecule to the brain.
1 . A composition comprising graphene oxide nanoparticles (GO) comprising a plurality of graphene sheets having a plurality of carboxylic and hydroxyl groups and interconvertible open and closed forms; the open form having a size of about 40 nm to 200 nm; the closed form having a diameter of about 2 nm to 120 nm; where the open form can be converted to the closed form at a pH of about 6.8 or lower; and the closed form can be converted to the open form at a pH of about 7.2 or higher
2 . The composition of claim 1 , wherein the carboxylic groups comprise at least about 20% of the total weight of the GO.
3 . The composition of claim 1 , wherein the GO has a solubility in aqueous solution at a concentration of about 1 mg GO/mL.
4 . The composition of claim 1 , wherein the GO displays fluorescence in the blue, green, red, and infrared spectra.
5 . The composition of claim 1 , wherein the GO has amphiphilic properties.
6 . A method for reversible encapsulation of a molecule within the GO of claim 1 , the method comprising:
contacting the open form GO with a molecule to be encapsulated;
converting the open form GO to the closed form GO with the molecule encapsulated therein by adjusting the pH to about 6.8 or lower; and
the molecule is be released from the closed form GO at a pH of about 7.2 or higher.
7 . The method of claim 6 , wherein the molecule is one or more of donepezil, tetraphenylporphyrin, a protein, a peptide, a small molecule, a nucleic acid, a single strand DNA, a double strand DNA, an RNA, an siRNA, an oligonucleotide, a gene, a gene fragment, an imaging agent, a lanthanide, or a combination thereof.
8 . A composition comprising a water-soluble carbon nanoparticle (wsCNP) having an encapsulated molecule therein, the wsCNP comprising a plurality of graphene sheets having a plurality of carboxylic and hydroxyl groups and interconvertible open and closed forms; the open form having a size of about 40 nm to 200 nm; the closed form having a diameter of about 2 nm to 120 nm; where the open form can be converted to the closed form at a pH of about 6.8 or lower; and the closed form can be converted to the open form at a pH of about 7.2 or higher
9 . The composition of claim 8 , wherein the encapsulated molecule is an imaging agent or a therapeutic agent.
10 . The composition of claim 8 , wherein the encapsulated molecule is a therapeutic agent for the treatment of a brain disorder.
11 . The composition of claim 8 , wherein the encapsulated molecule is one or more of donepezil, tetraphenylporphyrin, a protein, a peptide, a small molecule, a nucleic acid, a single strand DNA, a double strand DNA, an RNA, an siRNA, an oligonucleotide, a gene, a gene fragment, an imaging agent, a lanthanide, or a combination thereof.
12 . The composition of claim 8 , wherein the encapsulated molecule is donepezil or tetraphenylporphyrin.
13 . The composition of claim 8 , wherein the encapsulated molecule is a therapeutic agent for treatment of cancer.
14 . The composition of claim 8 , further comprising one or more biomolecules or divalent metals for targeted delivery of the wsCNP with the encapsulated molecule to a cell, tissue, brain, or organ.
15 . The composition of claim 14 , wherein the biomolecule is a protein, a receptor, an aptamer, a ligand, or an antibody; and the divalent metal is manganese (Mn).
16 . A method for delivering a water-soluble carbon nanoparticle (wsCNP) having an encapsulated molecule therein, the method comprising:
delivering to a subject a water-soluble carbon nanoparticle (wsCNP) having a molecule encapsulated therein, where the wsCNP comprises a plurality of graphene sheets having a plurality of carboxylic and hydroxyl groups and interconvertible open and closed forms; the open form having a size of about 40 nm to 200 nm; the closed form having a diameter of about 2 nm to 120 nm; where the open form can be converted to the closed form at a pH of about 6.8 or lower; and the closed form can be converted to the open form at a pH of about 7.2 or higher; and
the encapsulated molecule is released from the wsCNP at a pH of 7.4 after delivery.
17 . The method of claim 16 , wherein delivering comprises intravenous injection or inhalation.
18 . The method of claim 16 , wherein the molecule is one or a combination of donepezil, tetraphenylporphyrin, a protein, a peptide, a small molecule, a nucleic acid, a single strand DNA, a double strand DNA, an RNA, an siRNA, an oligonucleotide, a gene, a gene fragment, an imaging agent, or a lanthanide.
19 . The method of claim 16 , wherein the molecule is donepezil or tetraphenylporphyrin.
20 . The method of claim 16 , wherein the molecule is a therapeutic agent for the treatment of a brain disorder.
21 . The method of claim 20 , wherein the brain disorder comprises dementia, vascular dementia, or Alzheimer's.
22 . The method of claim 21 , wherein the molecule is a therapeutic agent for treatment of cancer.
23 . The method of claim 16 , wherein the molecule is delivered across the blood brain barrier (BBB).
24 . The method of claim 16 , wherein the wsCNP further comprises one or more biomolecules or divalent metals for targeted delivery of the wsCNP with the encapsulated molecule to a cell, tissue, brain, or organ.
25 . The method of claim 24 , wherein the biomolecule is a protein, a receptor, an aptamer, a ligand, or an aptamer; and the divalent metal is manganese (Mn).
26 . The method of claim 18 , wherein the wsCNP is produced by a process comprising:
(a) treating a material comprising one or a combination of wood, charcoal, low grade coal, or carbonized plant biomass with a dilute alkali solution to form a mixture of components including insoluble material and a second solution;
(b) separating the second solution from the insoluble material;
(c) neutralizing the second solution to form a precipitate; and
(d) separating the precipitate from the neutralized second solution;
wherein the precipitate comprises graphene oxide nanoparticles (GO) having a plurality of graphene sheets having a plurality of carboxylic and hydroxyl groups and interconvertible open and closed forms; the open form having a size of about 40 nm to 200 nm; the closed form having a diameter of about 2 nm to 120 nm; where the open form can be converted to the closed form at a pH of about 6.8 or lower;
and the closed form can be converted to the open form at a pH of about 7.2 or higher.