Manipulating and detecting biological samples
Disclosed herein, inter alia, are compositions and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates.
1. A system configured to form a section of biological tissue, comprising:
a cutting device forming a sharp edge;
a sample construct formed of a tissue section bonded to a carrier substrate, wherein the tissue section bonds to the carrier substrate via a first bond having a first adhesion strength; and
a receiving substrate;
wherein the sharp edge of the cutting device is configured to cut through both the tissue section and the carrier substrate of the sample construct to form a construct portion,
wherein the construct portion includes a portion of the tissue section and a portion of the carrier substrate, and
wherein the portion of the tissue section bonds to the receiving substrate via a second bond having a second adhesion strength, wherein the second adhesion strength is greater than the first adhesion strength.
2. The system of claim 1 , wherein the cutting device is a hole punch.
3. The system of claim 1 , wherein the cutting device is cylindrical.
4. The system of claim 1 , wherein the cutting device comprises a cutting blade.
5. The system of claim 1 , wherein the cutting device comprises a hollow structure.
6. The system of claim 1 , wherein the cutting device is circular.
7. The system of claim 1 , wherein a width of the cutting device about 1 mm to about 10 mm.
8. The system of claim 1 , wherein said tissue section comprises breast tissue, lung tissue, colon tissue, lymph tissue, kidney tissue, bone tissue, tonsil tissue, or brain tissue.
9. The system of claim 1 , further comprising a detection agent comprising a fluorophore, wherein the detection agent is bound to a molecule in the portion of the tissue section.
10. The system of claim 1 , wherein the carrier substrate comprises water molecules attached to a surface of the carrier substrate.
11. The system of claim 1 , wherein the carrier substrate comprises a compression modulus greater than about 100 kPa.
12. The system of claim 1 , wherein a thickness of the tissue section is about 1 μm to about 20 μm.
13. The system of claim 1 , wherein the carrier substrate comprises agarose, amylose, or amylopectin.
14. The system of claim 1 , wherein the receiving substrate is glass.
15. The system of claim 14 , wherein the system further comprises a plunger configured to expel the portion of the tissue section from the cutting device onto the receiving substrate.
16. The system of claim 14 , further comprising a plurality of fluorescent particles.
17. The system of claim 1 , wherein the receiving substrate comprises a functionalized glass surface.
18. The system of claim 1 , wherein the system further comprises a plunger configured to expel the portion of the tissue section from the cutting device.
19. The system of claim 1 , wherein the carrier substrate comprises gelatin.
20. The system of claim 1 , wherein the carrier substrate comprises agarose.
21. The system of claim 20 , wherein the carrier substrate comprises 2%, 3%, 4%, or 5% agarose.
22. The system of claim 1 , wherein the carrier substrate comprises a glycerol.
23. The system of claim 1 , wherein the tissue section is embedded in an embedding material comprising paraffin wax.
24. The system of claim 1 , wherein the second adhesion strength is 20% greater than the first adhesion strength.
25. The system of claim 1 , wherein the second adhesion strength is 80% greater than the first adhesion strength.
26. The system of claim 1 , wherein a thickness of the tissue section is about 5 μm to about 12 μm.
27. The system of claim 1 , wherein the cutting device is configured to form a square construct portion.
28. The system of claim 1 , wherein the cutting device is configured to form a circular construct portion.